71180

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 31852..32377 Replicon plasmid pEC656_1
Accession NZ_CP018978
Organism Escherichia coli strain Ecol_656

Toxin (Protein)


Gene name ccdB Uniprot ID V0SSI5
Locus tag BE941_RS00595 Protein ID WP_001159868.1
Coordinates 32072..32377 (+) Length 102 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID S1PPD8
Locus tag BE941_RS00590 Protein ID WP_000813634.1
Coordinates 31852..32070 (+) Length 73 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
BE941_RS00565 27131..28744 - 1614 WP_000080195.1 IS66-like element ISEc23 family transposase -
BE941_RS00570 28775..29125 - 351 WP_000624722.1 IS66 family insertion sequence element accessory protein TnpB -
BE941_RS00575 29122..29547 - 426 WP_000422741.1 IS66 family insertion sequence hypothetical protein -
BE941_RS00580 29609..30760 + 1152 WP_031325939.1 DUF3800 domain-containing protein -
BE941_RS00585 30794..31306 - 513 WP_000151784.1 hypothetical protein -
BE941_RS00590 31852..32070 + 219 WP_000813634.1 type II toxin-antitoxin system antitoxin CcdA Antitoxin
BE941_RS00595 32072..32377 + 306 WP_001159868.1 type II toxin-antitoxin system toxin CcdB Toxin
BE941_RS00600 32378..33184 + 807 WP_000016982.1 site-specific integrase -
BE941_RS00605 33958..34713 + 756 WP_000852146.1 replication initiation protein RepE -
BE941_RS00610 35301..36467 + 1167 WP_000772446.1 plasmid-partitioning protein SopA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid blaTEM-1B / tet(A) - 1..117844 117844


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-100)

Antitoxin

Post-segregation antitoxin CcdA

(2-72)


Sequences

Toxin        


Download         Length: 102 a.a.        Molecular weight: 11706.51 Da        Isoelectric Point: 6.4674

>T71180 WP_001159868.1 NZ_CP018978:32072-32377 [Escherichia coli]
MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKVSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEV
ADLSHRENDIKNAINLMFWGI

Download         Length: 306 bp

>T71180 NZ_CP018978:32072-32377 [Escherichia coli]
ATGCAGTTTAAGGTTTACACCTATAAAAGAGAGAGCCGTTATCGTCTGTTTGTGGATGTACAGAGTGATATTATTGACAC
GCCCGGGCGACGGATGGTGATCCCCCTGGCCAGTGCACGTCTGCTGTCAGATAAAGTCTCCCGTGAACTTTACCCGGTGG
TGCATATCGGGGATGAAAGCTGGCGCATGATGACCACCGATATGGCCAGTGTGCCGGTCTCCGTTATCGGGGAAGAAGTG
GCTGATCTCAGCCACCGCGAAAATGACATCAAAAACGCCATTAACCTGATGTTCTGGGGAATATAA

Antitoxin


Download         Length: 73 a.a.        Molecular weight: 8372.34 Da        Isoelectric Point: 4.7405

>AT71180 WP_000813634.1 NZ_CP018978:31852-32070 [Escherichia coli]
MKQRITVTVDSDSYQLLKAYDVNISGLVSTTMQNEARRLRAERWKAENQEGMAEVARFIEMNGSFADENRDW

Download         Length: 219 bp

>AT71180 NZ_CP018978:31852-32070 [Escherichia coli]
ATGAAGCAGCGTATTACAGTGACAGTTGACAGCGACAGCTATCAGTTGCTCAAGGCATATGATGTCAATATCTCCGGTCT
GGTAAGCACAACCATGCAGAATGAAGCCCGTCGTCTGCGTGCCGAACGCTGGAAAGCGGAAAATCAGGAAGGGATGGCTG
AGGTCGCCCGGTTTATTGAAATGAACGGCTCTTTTGCTGACGAGAACAGGGACTGGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4VUB
PDB 2VUB
PDB 7EPI
PDB 7EPG
PDB 3VUB
PDB 1X75
PDB 1VUB
PDB 7EPJ
PDB 3HPW
PDB 3G7Z

Antitoxin

Source ID Structure
AlphaFold DB A0A829CQY2

References