71177

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) relBE/RHH(antitoxin)
Location 12853..13412 Replicon plasmid pEC656_1
Accession NZ_CP018978
Organism Escherichia coli strain Ecol_656

Toxin (Protein)


Gene name relE Uniprot ID V0SVC8
Locus tag BE941_RS00475 Protein ID WP_000421272.1
Coordinates 13137..13412 (+) Length 92 a.a.

Antitoxin (Protein)


Gene name relB Uniprot ID V0SRR3
Locus tag BE941_RS00470 Protein ID WP_001387467.1
Coordinates 12853..13137 (+) Length 95 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
BE941_RS00440 8316..8756 - 441 Protein_10 zinc-binding dehydrogenase -
BE941_RS00450 9157..10047 + 891 WP_001077068.1 dihydrodipicolinate synthase family protein -
BE941_RS00455 10072..10452 + 381 WP_000710783.1 Rid family detoxifying hydrolase -
BE941_RS00460 10485..11450 + 966 WP_001022265.1 2-keto-3-deoxygluconate transporter -
BE941_RS00465 11496..12248 - 753 WP_000562172.1 IclR family transcriptional regulator -
BE941_RS00470 12853..13137 + 285 WP_001387467.1 ribbon-helix-helix domain-containing protein Antitoxin
BE941_RS00475 13137..13412 + 276 WP_000421272.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
BE941_RS00480 13518..13811 + 294 WP_001105060.1 hypothetical protein -
BE941_RS28415 13846..13974 - 129 Protein_18 IS481 family transposase -
BE941_RS00485 14007..15176 - 1170 WP_001072355.1 hypothetical protein -
BE941_RS00495 15669..15976 + 308 Protein_20 type II toxin-antitoxin system ParD family antitoxin -
BE941_RS00510 16265..16962 + 698 Protein_21 IS1 family transposase -
BE941_RS00520 17216..18238 - 1023 WP_000361402.1 helicase UvrD -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid blaTEM-1B / tet(A) - 1..117844 117844
- flank IS/Tn - - 16585..16962 377


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(3-90)

Antitoxin

Ribbon-helix-helix protein, CopG

(14-51)


Sequences

Toxin        


Download         Length: 92 a.a.        Molecular weight: 10749.45 Da        Isoelectric Point: 10.0871

>T71177 WP_000421272.1 NZ_CP018978:13137-13412 [Escherichia coli]
MELKWTSKALSDLSRLFDFLVLASKPAAARTVQSLTQAPVILLTHPRMGEQLFQFEPREVRRIFAGEYEIRYEINGQTIY
VLRLWHTRENR

Download         Length: 276 bp

>T71177 NZ_CP018978:13137-13412 [Escherichia coli]
ATGGAACTGAAGTGGACCAGTAAGGCGCTTTCTGATTTGTCGCGGTTATTTGATTTTCTGGTGCTGGCCAGTAAACCTGC
TGCCGCCAGAACGGTACAGTCCCTGACACAGGCACCGGTCATTCTGTTAACTCATCCACGTATGGGAGAACAGTTGTTTC
AGTTTGAACCCAGGGAGGTCAGACGGATTTTTGCTGGCGAGTACGAAATCCGTTACGAAATTAATGGCCAGACTATTTAT
GTATTGCGTCTGTGGCACACACGAGAAAACAGGTAG

Antitoxin


Download         Length: 95 a.a.        Molecular weight: 10460.87 Da        Isoelectric Point: 5.6558

>AT71177 WP_001387467.1 NZ_CP018978:12853-13137 [Escherichia coli]
MQMKNNAAQATKVITAHVPLPMADKVDQMAARLERSRGWVIKQALSAWLAQEEERNRLTLEALDDVTSGQVIDHQAVQAW
SDSLSTDNPLPVPR

Download         Length: 285 bp

>AT71177 NZ_CP018978:12853-13137 [Escherichia coli]
ATGCAAATGAAAAACAATGCCGCACAAGCAACAAAAGTAATTACCGCGCATGTGCCATTACCTATGGCTGATAAAGTCGA
CCAGATGGCCGCCAGACTGGAACGTTCCCGGGGCTGGGTTATCAAACAGGCGCTTTCTGCATGGCTTGCCCAGGAGGAGG
AGCGTAATCGCCTGACGCTGGAAGCCCTGGACGATGTGACATCCGGACAGGTTATCGACCATCAGGCTGTACAGGCCTGG
TCGGACAGCCTCAGTACTGACAATCCGTTACCGGTGCCACGCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB V0SVC8

Antitoxin

Source ID Structure
AlphaFold DB A0A0E0Y880

References