Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) yeeUV (cbtA-cbeA)/CbtA-CbeA
Location 1771162..1771993 Replicon chromosome
Accession NZ_CP018970
Organism Escherichia coli strain Ecol_542

Toxin (Protein)


Gene name yeeV Uniprot ID A0A066T988
Locus tag BE966_RS10820 Protein ID WP_000854815.1
Coordinates 1771619..1771993 (+) Length 125 a.a.

Antitoxin (Protein)


Gene name yeeU Uniprot ID A0A061Y7A8
Locus tag BE966_RS10815 Protein ID WP_001280918.1
Coordinates 1771162..1771530 (+) Length 123 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
BE966_RS10765 1766251..1766997 + 747 WP_001016257.1 IS21-like element ISEc12 family helper ATPase IstB -
BE966_RS10770 1767080..1767430 + 351 Protein_1810 hypothetical protein -
BE966_RS10775 1767446..1767856 + 411 WP_000846703.1 hypothetical protein -
BE966_RS10785 1768077..1768895 + 819 WP_001542275.1 DUF945 domain-containing protein -
BE966_RS10790 1768895..1769140 + 246 WP_001164966.1 hypothetical protein -
BE966_RS10795 1769234..1769707 + 474 WP_001542276.1 antirestriction protein -
BE966_RS10800 1769723..1770199 + 477 WP_001186200.1 RadC family protein -
BE966_RS10805 1770262..1770483 + 222 WP_000692345.1 DUF987 domain-containing protein -
BE966_RS10810 1770502..1771146 + 645 WP_000086752.1 hypothetical protein -
BE966_RS10815 1771162..1771530 + 369 WP_001280918.1 type IV toxin-antitoxin system YeeU family antitoxin Antitoxin
BE966_RS10820 1771619..1771993 + 375 WP_000854815.1 type IV toxin-antitoxin system toxin CbtA Toxin
BE966_RS10825 1771990..1772184 + 195 WP_000988600.1 hypothetical protein -
BE966_RS28805 1772197..1772310 + 114 WP_001161660.1 DUF957 domain-containing protein -
BE966_RS10830 1772599..1772745 - 147 Protein_1822 transposase domain-containing protein -
BE966_RS10835 1772799..1772981 + 183 WP_001296208.1 hypothetical protein -
BE966_RS10840 1773082..1773411 - 330 WP_000450409.1 DUF496 family protein -
BE966_RS10845 1773583..1774641 - 1059 WP_001200889.1 FUSC family protein -
BE966_RS10850 1774839..1775312 - 474 WP_001105368.1 DNA gyrase inhibitor SbmC -
BE966_RS10855 1775431..1776597 - 1167 WP_001296209.1 serine-type D-Ala-D-Ala carboxypeptidase DacD -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

CbtA toxin of type IV toxin-antitoxin system

(1-112)

Antitoxin

Antitoxin CbeA

(19-117)


Sequences

Toxin        


Download         Length: 125 a.a.        Molecular weight: 13829.83 Da        Isoelectric Point: 7.1326

>T71126 WP_000854815.1 NZ_CP018970:1771619-1771993 [Escherichia coli]
MKTLPVLPGQAASSRPSPVEIWQILLSRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
SACTRSQLINSIDILRARRATGLMTSDNYRTVNNITLGKYPEAK

Download         Length: 375 bp

>T71126 NZ_CP018970:1771619-1771993 [Escherichia coli]
ATGAAAACATTACCTGTATTACCCGGGCAGGCGGCCAGTTCTCGCCCGTCTCCTGTTGAAATCTGGCAGATACTGCTGTC
CCGACTGCTGGACCAGCACTATGGCCTCACACTGAATGACACACCGTTTGCCGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGCATTTCACTGTGTGATGCGGTGAACTTTCTCGTGGAAAAATACGCGCTGGTGCGTACCGACCAGCCGGGATTC
AGCGCCTGTACCCGCTCTCAGTTAATAAACAGCATCGATATCCTCCGGGCTCGCAGGGCGACCGGCCTGATGACCAGCGA
CAATTACAGAACGGTAAATAACATTACCCTGGGTAAGTATCCGGAGGCGAAATGA

Antitoxin


Download         Length: 123 a.a.        Molecular weight: 13811.70 Da        Isoelectric Point: 6.4767

>AT71126 WP_001280918.1 NZ_CP018970:1771162-1771530 [Escherichia coli]
VSDKLHETNYPDDHNDRLWWGLPCTVTPCFGARLVQEGNRLHYLADRAGIRGLFSDADAYHLDQAFPLLMKQLELMLTSG
ELNPRHQHTVTLYAKGLTCKADTLSSCGYVYLAVYPTPEMKN

Download         Length: 369 bp

>AT71126 NZ_CP018970:1771162-1771530 [Escherichia coli]
GTGTCAGACAAACTCCATGAGACAAATTATCCCGACGACCATAACGACCGCCTCTGGTGGGGACTGCCCTGCACCGTGAC
GCCCTGTTTCGGGGCACGTCTGGTGCAGGAGGGTAACCGGTTGCATTACCTTGCCGACCGCGCCGGTATCAGAGGCCTGT
TCAGCGATGCAGATGCGTACCACCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAACTCATGCTCACCAGCGGT
GAACTGAATCCCCGCCATCAGCATACCGTCACGCTGTATGCAAAAGGGCTGACCTGCAAAGCCGATACCCTCAGCAGTTG
TGGTTACGTTTATCTGGCTGTTTATCCGACGCCCGAAATGAAAAATTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A066T988

Antitoxin

Source ID Structure
AlphaFold DB A0A061Y7A8

References