Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) phd-doc/Doc-Phd
Location 52032..52633 Replicon plasmid pEC542_1
Accession NZ_CP018969
Organism Escherichia coli strain Ecol_542

Toxin (Protein)


Gene name doc Uniprot ID V0AJ64
Locus tag BE966_RS00720 Protein ID WP_001216034.1
Coordinates 52253..52633 (+) Length 127 a.a.

Antitoxin (Protein)


Gene name phd Uniprot ID U9YQH9
Locus tag BE966_RS00715 Protein ID WP_001190712.1
Coordinates 52032..52253 (+) Length 74 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
BE966_RS00705 49013..50296 - 1284 WP_001617890.1 restriction endonuclease subunit S -
BE966_RS00710 50293..51849 - 1557 WP_001617892.1 type I restriction-modification system subunit M -
BE966_RS00715 52032..52253 + 222 WP_001190712.1 type II toxin-antitoxin system Phd/YefM family antitoxin Antitoxin
BE966_RS00720 52253..52633 + 381 WP_001216034.1 type II toxin-antitoxin system death-on-curing family toxin Toxin
BE966_RS00725 52638..52817 + 180 WP_001513661.1 hypothetical protein -
BE966_RS00730 52845..53204 + 360 WP_001513660.1 hypothetical protein -
BE966_RS00735 53128..53541 + 414 Protein_67 DDE-type integrase/transposase/recombinase -
BE966_RS00740 53491..53808 - 318 WP_001513659.1 hypothetical protein -
BE966_RS00745 54036..55016 - 981 WP_000019407.1 IS5-like element IS5 family transposase -
BE966_RS00750 55260..56663 + 1404 WP_001373486.1 S-methylmethionine permease -
BE966_RS00755 56650..57582 + 933 WP_000081352.1 homocysteine S-methyltransferase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Mobilizable plasmid blaCTX-M-27 - 1..82364 82364


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Death on curing protein

(2-123)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-51)


Sequences

Toxin        


Download         Length: 127 a.a.        Molecular weight: 13615.32 Da        Isoelectric Point: 5.1514

>T71111 WP_001216034.1 NZ_CP018969:52253-52633 [Escherichia coli]
MRHISPEELIALHDANINRYGGLPGMSDPGRAEAIIGRVQARVAYEEITDLFEVSATYLVATARGHIFNDANKRTALNSA
LLFLRRNGVQVFDSPELADLTVGAATGEISVSSVADTLRRLYGSAE

Download         Length: 381 bp

>T71111 NZ_CP018969:52253-52633 [Escherichia coli]
ATGAGGCATATATCACCGGAAGAACTTATTGCGCTTCATGATGCGAATATAAACCGCTACGGCGGCCTGCCGGGAATGTC
TGATCCGGGCAGGGCAGAGGCCATTATCGGGAGAGTTCAGGCCAGAGTTGCCTACGAAGAGATCACCGACCTTTTCGAAG
TCTCCGCCACCTACCTGGTGGCTACAGCGAGAGGGCATATATTCAATGATGCCAATAAGCGTACCGCGCTAAACAGTGCG
CTGTTATTTCTACGCCGTAACGGGGTGCAGGTATTTGATTCACCTGAACTGGCAGACCTTACCGTAGGGGCTGCGACCGG
AGAGATATCTGTATCTTCTGTCGCCGACACGTTACGTAGATTGTATGGTTCTGCGGAGTAG

Antitoxin


Download         Length: 74 a.a.        Molecular weight: 8133.10 Da        Isoelectric Point: 4.7708

>AT71111 WP_001190712.1 NZ_CP018969:52032-52253 [Escherichia coli]
MQSINFRTARGNLSEVLNNVEAGEEVEITRRGREPAVIVSKATFEAYKKAALDAEFASLFDTLDSTNKELVNR

Download         Length: 222 bp

>AT71111 NZ_CP018969:52032-52253 [Escherichia coli]
ATGCAATCCATTAACTTCCGTACCGCGCGCGGCAACCTTTCTGAAGTGCTCAACAATGTTGAGGCCGGGGAAGAGGTTGA
AATCACCCGCAGAGGCCGTGAGCCAGCAGTAATTGTCAGCAAGGCTACTTTCGAAGCCTACAAAAAAGCGGCGCTGGATG
CTGAATTTGCATCCCTGTTTGACACCCTGGACTCCACCAACAAGGAACTGGTTAACCGATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB V0AJ64

Antitoxin

Source ID Structure
AlphaFold DB A0A829CJB6

References