Detailed information of TA system TA071096

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 3282895..3283625
• Replicon: chromosome
• Accession: NZ_CP018965
• Organism: Escherichia coli strain Ecol_517

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: A0A0Q2Y5N3
• Locus tag: BE959_RS17855
• Protein ID: WP_001514886.1
• Coordinates: 3282895..3283269 (-)
• Length: 125 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: -
• Locus tag: BE959_RS17860
• Protein ID: WP_078230669.1
• Coordinates: 3283359..3283625 (-)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BE959_RS17820	3278799..3279977	+	1179	Protein_3025	site-specific integrase	-
BE959_RS17825	3279958..3280149	-	192	WP_000132739.1	AlpA family phage regulatory protein	-
BE959_RS17830	3280254..3280433	-	180	WP_053908461.1	Eag protein	-
BE959_RS17835	3280437..3281027	+	591	Protein_3028	metallophosphoesterase	-
BE959_RS17840	3281024..3281647	+	624	WP_053908465.1	antitermination protein	-
BE959_RS26160	3282566..3282679	-	114	WP_001161660.1	DUF957 domain-containing protein	-
BE959_RS17850	3282692..3282898	-	207	WP_000976829.1	hypothetical protein	-
BE959_RS17855	3282895..3283269	-	375	WP_001514886.1	TA system toxin CbtA family protein	Toxin
BE959_RS17860	3283359..3283625	-	267	WP_078230669.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
BE959_RS17870	3284081..3284650	+	570	WP_000221529.1	inovirus Gp2 family protein	-
BE959_RS17875	3284816..3285208	+	393	WP_000271005.1	hypothetical protein	-
BE959_RS17880	3285218..3286186	-	969	WP_038349051.1	IS110 family transposase	-
BE959_RS17885	3286372..3286782	+	411	WP_001534064.1	zinc-ribbon domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (1-83)

Sequences

Toxin

Protein

• Length: 125 a.a.
• Molecular weight: 13930.91 Da
• Isoelectric Point: 7.2909

>T71096 WP_001514886.1 NZ_CP018965:c3283269-3282895 [Escherichia coli]
MKTLPDTHVREVSCCPSPVTIWQTLLTRLLDQHYGLTLNDTPFVDERVIEQHIEAGISLCDAVNFLVEKYALVRTDQSGF
SAGASSQLINSIDILRARRATGLMTRHNYRTVNNITLGKYPEAK

Nucleotide

• Length: 375 bp

>T71096 NZ_CP018965:c3283269-3282895 [Escherichia coli]
ATGAAAACATTACCCGACACGCATGTACGGGAGGTATCGTGCTGCCCGTCTCCCGTCACCATCTGGCAGACACTGCTCAC
CCGACTGCTGGACCAGCACTACGGCCTTACGCTGAATGACACACCGTTCGTGGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGCATTTCACTGTGTGACGCGGTGAACTTTCTCGTTGAAAAATACGCGCTGGTACGTACCGACCAGTCGGGATTC
AGCGCAGGAGCCTCGTCGCAGTTAATCAACAGCATTGATATTCTCCGGGCTCGCCGGGCAACAGGCCTGATGACCCGCCA
CAACTACAGAACGGTGAACAATATCACCCTGGGTAAGTATCCGGAGGCGAAATGA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 9916.36 Da
• Isoelectric Point: 7.8720

>AT71096 WP_078230669.1 NZ_CP018965:c3283625-3283359 [Escherichia coli]
MQNGNRLHYLADRAGIRGRFRDADAYPLDQAFPLLMKQLELMLTSGELNPRHQHTVTLYAKGLTCEADTLGSCGYVYLAV
YPTPETKK

Nucleotide

• Length: 267 bp

>AT71096 NZ_CP018965:c3283625-3283359 [Escherichia coli]
CTGCAAAATGGTAACCGGTTGCATTACCTTGCCGACCGTGCCGGTATCAGAGGCCGGTTCAGAGATGCGGATGCGTACCC
TCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAATTAATGCTCACCAGTGGTGAACTGAATCCCCGCCATCAGC
ATACCGTGACACTGTATGCGAAGGGGCTGACCTGTGAAGCCGACACCCTCGGCTCCTGTGGTTACGTTTATCTGGCTGTT
TATCCGACACCTGAAACGAAAAAGTAA

Structures

Toxin

• AlphaFold DB: A0A0Q2Y5N3