Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 104513..105038 Replicon plasmid pEC517_1
Accession NZ_CP018964
Organism Escherichia coli strain Ecol_517

Toxin (Protein)


Gene name ccdB Uniprot ID S1PFV8
Locus tag BE959_RS01075 Protein ID WP_001159871.1
Coordinates 104733..105038 (+) Length 102 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID H9TJP1
Locus tag BE959_RS01070 Protein ID WP_000813630.1
Coordinates 104513..104731 (+) Length 73 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
BE959_RS01030 99723..100409 + 687 Protein_119 IS1 family transposase -
BE959_RS01040 100663..101685 - 1023 WP_000361402.1 helicase UvrD -
BE959_RS01045 101670..103235 - 1566 WP_001128474.1 AAA family ATPase -
BE959_RS01050 103310..103726 - 417 WP_001034044.1 type II toxin-antitoxin system VapC family toxin -
BE959_RS01055 103723..103953 - 231 WP_001261286.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein -
BE959_RS01070 104513..104731 + 219 WP_000813630.1 type II toxin-antitoxin system antitoxin CcdA Antitoxin
BE959_RS01075 104733..105038 + 306 WP_001159871.1 type II toxin-antitoxin system toxin CcdB Toxin
BE959_RS01080 105039..105845 + 807 WP_000016970.1 site-specific integrase -
BE959_RS01085 106567..107322 + 756 WP_000852146.1 replication initiation protein RepE -
BE959_RS01090 107910..109076 + 1167 WP_000772446.1 plasmid-partitioning protein SopA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid tet(A) / aac(6')-Ib-cr / blaOXA-1 / blaCTX-M-15 / mph(A) / sul1 / qacE / aadA5 senB / iucD / iutA 1..118495 118495


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-100)

Antitoxin

Post-segregation antitoxin CcdA

(2-72)


Sequences

Toxin        


Download         Length: 102 a.a.        Molecular weight: 11692.49 Da        Isoelectric Point: 6.4674

>T71084 WP_001159871.1 NZ_CP018964:104733-105038 [Escherichia coli]
MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKVSRELYPVVHVGDESWRMMTTDMASVPVSVIGEEV
ADLSHRENDIKNAINLMFWGI

Download         Length: 306 bp

>T71084 NZ_CP018964:104733-105038 [Escherichia coli]
ATGCAGTTTAAGGTTTACACCTATAAAAGAGAGAGCCGTTATCGTCTGTTTGTGGATGTCCAGAGTGACATTATTGACAC
GCCTGGGCGACGGATGGTGATCCCCCTGGCCAGTGCACGTCTGTTGTCAGATAAGGTCTCCCGTGAGCTTTACCCGGTGG
TGCATGTCGGGGATGAAAGCTGGCGCATGATGACCACCGATATGGCCAGTGTGCCGGTCTCCGTTATCGGGGAGGAAGTG
GCTGATCTCAGCCACCGAGAAAATGACATCAAAAATGCCATTAACCTGATGTTCTGGGGAATATAA

Antitoxin


Download         Length: 73 a.a.        Molecular weight: 8354.27 Da        Isoelectric Point: 4.5203

>AT71084 WP_000813630.1 NZ_CP018964:104513-104731 [Escherichia coli]
MKQRITVTVDSDSYQLLKAYDVNISGLVSTTLQNEARRLRAERWQAENQEGMAEVARFIEMNGSFADENRDW

Download         Length: 219 bp

>AT71084 NZ_CP018964:104513-104731 [Escherichia coli]
ATGAAGCAGCGCATTACAGTCACCGTCGACAGTGACAGCTATCAGTTGCTCAAGGCATATGATGTCAATATCTCCGGGCT
GGTAAGCACCACCCTGCAGAATGAAGCCCGTCGTCTGCGTGCCGAACGCTGGCAGGCAGAAAATCAGGAAGGGATGGCTG
AGGTCGCCCGGTTTATTGAAATGAACGGCTCTTTTGCCGACGAGAACAGGGACTGGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829CCE8

Antitoxin

Source ID Structure
AlphaFold DB A0A829CEF5

References