Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) yeeUV (cbtA-cbeA)/CbtA-CbeA
Location 4375042..4375873 Replicon chromosome
Accession NZ_CP018957
Organism Escherichia coli strain Ecol_316

Toxin (Protein)


Gene name yeeV Uniprot ID S1P968
Locus tag BE963_RS25190 Protein ID WP_000854814.1
Coordinates 4375499..4375873 (+) Length 125 a.a.

Antitoxin (Protein)


Gene name yeeU Uniprot ID B3Y195
Locus tag BE963_RS25185 Protein ID WP_001285585.1
Coordinates 4375042..4375410 (+) Length 123 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
BE963_RS25145 4371028..4371870 + 843 Protein_4080 hypothetical protein -
BE963_RS25150 4371946..4372401 + 456 WP_000581504.1 hypothetical protein -
BE963_RS25155 4372480..4372713 + 234 WP_001119729.1 DUF905 family protein -
BE963_RS25165 4372813..4373631 + 819 WP_021538423.1 DUF945 domain-containing protein -
BE963_RS25170 4373713..4374192 + 480 WP_000860087.1 antirestriction protein -
BE963_RS25175 4374208..4374684 + 477 WP_001186726.1 RadC family protein -
BE963_RS25180 4374747..4374968 + 222 WP_000692323.1 DUF987 domain-containing protein -
BE963_RS25185 4375042..4375410 + 369 WP_001285585.1 type IV toxin-antitoxin system toxin CbtA Antitoxin
BE963_RS25190 4375499..4375873 + 375 WP_000854814.1 type IV toxin-antitoxin system toxin CbtA Toxin
BE963_RS25195 4375870..4376064 + 195 WP_000988600.1 hypothetical protein -
BE963_RS28870 4376077..4376190 + 114 WP_001161660.1 DUF957 domain-containing protein -
BE963_RS25200 4376479..4376607 - 129 Protein_4091 transposase domain-containing protein -
BE963_RS25205 4376679..4376861 + 183 WP_032140263.1 ethanolamine utilization protein -
BE963_RS25210 4376962..4377291 - 330 WP_000450409.1 DUF496 family protein -
BE963_RS25215 4377463..4378521 - 1059 WP_001200891.1 FUSC family protein -
BE963_RS25220 4378719..4379192 - 474 WP_001105389.1 DNA gyrase inhibitor SbmC -
BE963_RS25225 4379311..4380477 - 1167 WP_000830150.1 serine-type D-Ala-D-Ala carboxypeptidase DacD -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

CbtA toxin of type IV toxin-antitoxin system

(1-112)

Antitoxin

Antitoxin CbeA

(18-117)


Sequences

Toxin        


Download         Length: 125 a.a.        Molecular weight: 13898.94 Da        Isoelectric Point: 7.8522

>T71078 WP_000854814.1 NZ_CP018957:4375499-4375873 [Escherichia coli]
MKTLPVLPGQAASSRPSPVEIWQILLSRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
SACTRSQLINSIDILRARRATGLMTRDNYRTVNNITLGKYPEAK

Download         Length: 375 bp

>T71078 NZ_CP018957:4375499-4375873 [Escherichia coli]
ATGAAAACATTACCTGTATTACCCGGGCAGGCGGCCAGTTCTCGCCCGTCTCCTGTTGAAATCTGGCAGATACTGCTGTC
CCGACTGCTGGACCAGCACTATGGCCTCACACTGAATGACACACCTTTTGCCGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGCATTTCACTGTGTGATGCGGTGAACTTTCTCGTGGAAAAATACGCGCTGGTGCGTACCGACCAGCCGGGATTC
AGCGCCTGTACCCGCTCTCAGTTAATAAACAGTATTGATATCCTCCGGGCACGCCGGGCAACCGGCCTGATGACCCGCGA
CAATTACAGAACGGTAAATAACATTACCCTGGGTAAGTATCCGGAGGCGAAATGA

Antitoxin


Download         Length: 123 a.a.        Molecular weight: 13593.48 Da        Isoelectric Point: 6.3139

>AT71078 WP_001285585.1 NZ_CP018957:4375042-4375410 [Escherichia coli]
VSDTLPGTTLPDDNHDRPWWGLPCTVTPCFGARLVQEGNRLHYLADRAGIRGLFSDADAYHLDQAFPLLMKQLELMLTSG
ELNPRHQHTVTLYAKGLTCKADTLSSCGYVYLAVYPTPEMKN

Download         Length: 369 bp

>AT71078 NZ_CP018957:4375042-4375410 [Escherichia coli]
GTGTCAGACACACTCCCCGGGACAACACTTCCCGACGACAATCACGACCGCCCCTGGTGGGGGCTGCCCTGCACCGTGAC
GCCCTGTTTCGGGGCACGTCTGGTGCAGGAGGGTAACCGGTTGCATTACCTTGCCGACCGCGCCGGTATCAGAGGCCTGT
TCAGCGATGCAGATGCGTACCACCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAACTCATGCTCACCAGCGGT
GAACTGAATCCCCGCCATCAGCATACCGTCACGCTGTATGCAAAAGGGCTGACCTGCAAAGCCGATACCCTCAGCAGTTG
TGGTTACGTTTATCTGGCTGTTTATCCGACGCCCGAAATGAAAAATTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829FY50

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9LW60

References