71067

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/UPF0150(antitoxin)
Location 3720380..3721018 Replicon chromosome
Accession NZ_CP018957
Organism Escherichia coli strain Ecol_316

Toxin (Protein)


Gene name hicA Uniprot ID D3GRG9
Locus tag BE963_RS21720 Protein ID WP_001314712.1
Coordinates 3720380..3720556 (+) Length 59 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID -
Locus tag BE963_RS21725 Protein ID WP_077248684.1
Coordinates 3720602..3721018 (+) Length 139 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
BE963_RS21700 3716002..3717213 - 1212 WP_078175070.1 BenE family transporter YdcO -
BE963_RS21705 3717266..3717802 + 537 WP_000429149.1 DNA-binding transcriptional regulator SutR -
BE963_RS21710 3717875..3719836 + 1962 WP_001587031.1 23S rRNA 5-hydroxycytidine C2501 synthase -
BE963_RS21715 3719928..3720158 - 231 WP_000494241.1 YncJ family protein -
BE963_RS21720 3720380..3720556 + 177 WP_001314712.1 type II toxin-antitoxin system mRNA interferase toxin HicA Toxin
BE963_RS21725 3720602..3721018 + 417 WP_077248684.1 type II toxin-antitoxin system antitoxin HicB Antitoxin
BE963_RS21730 3721097..3722503 + 1407 WP_000760623.1 PLP-dependent aminotransferase family protein -
BE963_RS21735 3722748..3723893 + 1146 WP_000047443.1 ABC transporter substrate-binding protein -
BE963_RS21740 3723911..3724924 + 1014 WP_000220407.1 ABC transporter ATP-binding protein -
BE963_RS21745 3724925..3725866 + 942 WP_001587036.1 ABC transporter permease -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(5-57)

Antitoxin

Cro/C1-type helix-turn-helix domain

(87-134)


Sequences

Toxin        


Download         Length: 59 a.a.        Molecular weight: 6746.81 Da        Isoelectric Point: 11.8888

>T71067 WP_001314712.1 NZ_CP018957:3720380-3720556 [Escherichia coli]
VKQSEFRRWLESQGVNVANGSNHLKLRFHGRRSVMPRHPSEEIKEPLRKAILKQLGLS

Download         Length: 177 bp

>T71067 NZ_CP018957:3720380-3720556 [Escherichia coli]
GTGAAACAAAGCGAGTTCAGACGTTGGCTCGAATCTCAGGGCGTCAATGTAGCAAATGGCAGCAACCATTTAAAACTCAG
GTTTCACGGGAGGCGCAGTGTCATGCCGCGTCACCCCAGCGAAGAGATTAAAGAACCATTACGTAAAGCCATTCTGAAAC
AACTCGGTTTGAGTTAA

Antitoxin


Download         Length: 139 a.a.        Molecular weight: 15232.60 Da        Isoelectric Point: 4.7286

>AT71067 WP_077248684.1 NZ_CP018957:3720602-3721018 [Escherichia coli]
MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALLTAFDFYFEDNELIPLPSPLNSHDHFIEVPLSVASKV
LLLNAFLQSDITQQELARRIGKPKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV

Download         Length: 417 bp

>AT71067 NZ_CP018957:3720602-3721018 [Escherichia coli]
ATGCGTTATCCCGTCACTCTTACACCCGCGCCGGAAGGCGGTTATATGGTTTCTTTTGTGGATATCCCTGAAGCGTTGAC
GCAGGGCGAAACTGTCGCTGAAGCGATGGAAGCGGCAAAAGATGCTTTACTGACCGCATTTGATTTTTATTTTGAAGATA
ACGAGCTTATCCCTTTACCTTCGCCATTAAATAGTCACGATCACTTTATTGAAGTGCCTTTGAGCGTCGCCTCTAAGGTA
TTGCTGTTAAATGCTTTTTTACAGTCAGACATCACTCAGCAAGAATTAGCCAGGCGAATTGGCAAACCTAAACAGGAAAT
TACTCGCTTATTTAACTTGCACCATGCGACAAAAATCGACGCCGTCCAGCTTGCGGCTAAGGCGCTTGGCAAAGAGTTAT
CGCTGGTGATGGTTTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB D3GRG9

Antitoxin

Source ID Structure

References