Detailed information of TA system TA071055

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mosAT/AbiEii-AbiEi
• Location: 2435922..2437575
• Replicon: chromosome
• Accession: NZ_CP018957
• Organism: Escherichia coli strain Ecol_316

Toxin (Protein)

• Gene name: mosT
• Uniprot ID: A0A0X5EJM1
• Locus tag: BE963_RS15025
• Protein ID: WP_001615045.1
• Coordinates: 2436691..2437575 (+)
• Length: 295 a.a.

Antitoxin (Protein)

• Gene name: mosA
• Uniprot ID: -
• Locus tag: BE963_RS15020
• Protein ID: WP_001134467.1
• Coordinates: 2435922..2436704 (+)
• Length: 261 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BE963_RS14995	2431375..2432700	-	1326	WP_001586702.1	DNA phosphorothioation-dependent restriction protein DptG	-
BE963_RS15000	2432705..2434354	-	1650	WP_001586703.1	DNA phosphorothioation-dependent restriction protein DptF	-
BE963_RS15005	2434631..2434855	-	225	WP_024187424.1	helix-turn-helix domain-containing protein	-
BE963_RS15010	2434936..2435166	-	231	WP_001577779.1	helix-turn-helix domain-containing protein	-
BE963_RS15015	2435235..2435545	+	311	Protein_2235	helix-turn-helix domain-containing protein	-
BE963_RS15020	2435922..2436704	+	783	WP_001134467.1	type IV toxin-antitoxin system AbiEi family antitoxin domain-containing protein	Antitoxin
BE963_RS15025	2436691..2437575	+	885	WP_001615045.1	nucleotidyl transferase AbiEii/AbiGii toxin family protein	Toxin
BE963_RS15035	2438027..2438911	-	885	WP_001119037.1	LysR family transcriptional regulator	-
BE963_RS15040	2439176..2440234	+	1059	WP_001586705.1	alpha/beta hydrolase	-
BE963_RS15045	2440376..2441503	+	1128	WP_000171079.1	MFS transporter	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	yagV/ecpE / yagW/ecpD / yagX/ecpC / yagY/ecpB / yagZ/ecpA / ykgK/ecpR / fdeC	2424236..2478737	54501

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Nucleotidyl transferase AbiEii toxin, Type IV TA system (14-209)

Antitoxin

• Transcriptional regulator AbiEi antitoxin, N-terminal domain (3-90)

Sequences

Toxin

Protein

• Length: 295 a.a.
• Molecular weight: 33151.42 Da
• Isoelectric Point: 7.7408

>T71055 WP_001615045.1 NZ_CP018957:2436691-2437575 [Escherichia coli]
MDLRDSYARQVKLLMAALPHVAKESCFALKGGTAINLFVQDFPRLSVDIDLAAINDALKRITASLNGRPGITAIRQENKA
DEKRIIVNTADAKIKIEVSPVWRGLLLPPAEMPVCERVEMEYGFTTMSVVSLADLYGGKICAALDRQHPRDLFDVLNMLE
KPGVMREIFDGFLCYLAGHPRPIAELLAPNWDTERITTLYAQEFSGMTQQETSLESLLSVTTLLPQALKSHLTDLDREFS
LSFKQNSPDWSRYRYPEIQHLPAIRWKQRNLAVLKDKNPAKYVAAVNKLERVLA

Nucleotide

• Length: 885 bp

>T71055 NZ_CP018957:2436691-2437575 [Escherichia coli]
ATGGATCTGCGTGATAGTTATGCCCGGCAGGTTAAGCTGTTGATGGCTGCTCTGCCGCATGTGGCGAAGGAGTCCTGTTT
CGCGTTGAAAGGCGGCACGGCAATCAATTTGTTTGTGCAGGATTTTCCCCGTCTGTCGGTGGATATTGATCTGGCGGCTA
TCAACGACGCTTTGAAGAGGATTACTGCATCGCTGAACGGCAGACCGGGGATCACCGCGATTCGGCAGGAAAATAAAGCG
GATGAAAAGCGCATCATTGTGAATACCGCTGATGCAAAGATTAAGATCGAGGTTAGCCCGGTCTGGCGCGGGCTGTTGTT
GCCTCCGGCAGAAATGCCTGTGTGCGAACGTGTGGAAATGGAGTATGGCTTCACCACCATGAGTGTCGTCTCGCTGGCGG
ATCTTTATGGCGGTAAAATCTGCGCAGCGCTTGATCGTCAGCACCCGCGCGATCTGTTTGATGTGCTGAATATGCTGGAG
AAACCGGGAGTGATGCGGGAGATCTTTGACGGTTTTCTTTGTTATCTGGCAGGCCATCCCCGCCCCATTGCCGAGTTGCT
GGCCCCAAACTGGGATACAGAGCGGATCACGACGCTGTATGCTCAGGAATTTTCGGGGATGACTCAGCAGGAAACGTCGC
TGGAATCGCTGCTGTCAGTAACGACTTTGCTGCCGCAGGCGTTGAAGTCACATCTTACGGATCTGGATCGCGAGTTCTCG
CTCAGTTTTAAGCAAAACAGCCCTGACTGGTCGCGGTATCGCTATCCTGAAATTCAGCATCTTCCGGCTATTCGCTGGAA
GCAACGTAATCTGGCGGTGCTGAAAGATAAAAATCCGGCGAAGTATGTGGCGGCGGTGAATAAGCTAGAGAGGGTGCTGG
CGTAA

Antitoxin

Protein

• Length: 261 a.a.
• Molecular weight: 29093.39 Da
• Isoelectric Point: 8.4395

>AT71055 WP_001134467.1 NZ_CP018957:2435922-2436704 [Escherichia coli]
MPNYLNWLMQHTLPGQVLLQSWLTRNGIDRKLSYLYVKNGWLRRIAHGVYCRTGREPDWVDAVYCLQTQWEKPVRVAGLT
SLALQGHAHYTEPGKPQVWLALPAYVKLPGWFAAFEQSATFITLYTSGLTVDVTSLTTELKIGDRQLAGSAPELAAYEIA
CGVPSLISFEHADALFQGMNLLSPRKLQALLCASHSVKTNRVMLYLARRNGHPYFQYIDQSGIETGTGKRQIIPGGWLEP
DYQITVPRAFKPEGVEDGSA

Nucleotide

• Length: 783 bp

>AT71055 NZ_CP018957:2435922-2436704 [Escherichia coli]
TTGCCAAACTATCTAAACTGGCTGATGCAGCACACGCTACCCGGCCAGGTGCTGCTCCAGTCATGGCTTACCCGCAACGG
CATAGATCGTAAGCTCTCTTATCTCTATGTGAAGAATGGCTGGCTCAGGCGGATCGCTCACGGCGTATACTGCCGGACGG
GCCGTGAGCCGGATTGGGTAGACGCGGTTTACTGCCTTCAAACACAGTGGGAAAAGCCTGTTCGGGTGGCGGGGCTAACC
AGCCTTGCGCTTCAGGGCCATGCGCACTATACCGAACCGGGTAAGCCGCAGGTCTGGCTCGCGCTTCCGGCTTACGTTAA
GCTGCCGGGATGGTTTGCTGCCTTCGAACAATCTGCAACATTTATCACGCTGTATACATCGGGGCTTACCGTAGATGTAA
CGTCATTGACTACGGAACTGAAAATTGGCGACCGCCAGTTAGCAGGCAGTGCCCCGGAACTGGCCGCGTATGAGATCGCC
TGCGGCGTTCCTTCGTTGATATCGTTCGAACATGCCGATGCTCTGTTTCAGGGGATGAATCTTCTCAGCCCGCGTAAATT
GCAGGCGTTACTCTGCGCCAGTCATTCGGTTAAAACCAACCGGGTGATGCTTTACCTTGCCCGGCGTAATGGACACCCTT
ATTTTCAGTATATTGATCAAAGCGGTATAGAAACAGGTACGGGAAAGCGGCAAATCATACCCGGCGGCTGGCTGGAGCCG
GATTATCAAATTACTGTTCCACGCGCATTTAAACCGGAAGGAGTCGAAGATGGATCTGCGTGA

Structures

Toxin

• AlphaFold DB: A0A0X5EJM1