Detailed information of TA system TA071053

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): ccdAB/CcdA(antitoxin)
• Location: 2175476..2175900
• Replicon: chromosome
• Accession: NZ_CP018957
• Organism: Escherichia coli strain Ecol_316

Toxin (Protein)

• Gene name: ccdB
• Uniprot ID: -
• Locus tag: BE963_RS29400
• Protein ID: WP_001586653.1
• Coordinates: 2175712..2175900 (+)
• Length: 63 a.a.

Antitoxin (Protein)

• Gene name: ccdA
• Uniprot ID: V0VEX8
• Locus tag: BE963_RS13805
• Protein ID: WP_000125566.1
• Coordinates: 2175476..2175709 (+)
• Length: 78 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BE963_RS13780	2170549..2170836	+	288	WP_000203747.1	ferredoxin-like protein FixX	-
BE963_RS13785	2170895..2172226	+	1332	WP_001183198.1	MFS transporter	-
BE963_RS13790	2172334..2172864	+	531	WP_000600725.1	glutathione-regulated potassium-efflux system oxidoreductase KefF	-
BE963_RS13795	2172857..2174719	+	1863	WP_000377113.1	glutathione-regulated potassium-efflux system protein KefC	-
BE963_RS13800	2174911..2175390	+	480	WP_000624375.1	type 3 dihydrofolate reductase	-
BE963_RS13805	2175476..2175709	+	234	WP_000125566.1	type II toxin-antitoxin system CcdA family antitoxin	Antitoxin
BE963_RS29400	2175712..2175900	+	189	WP_001586653.1	CcdB family protein	Toxin
BE963_RS29405	2175897..2176013	+	117	WP_001389634.1	CcdB family protein	-
BE963_RS13815	2176010..2176858	-	849	WP_000257195.1	bis(5'-nucleosyl)-tetraphosphatase (symmetrical) ApaH	-
BE963_RS13820	2176865..2177242	-	378	WP_000610901.1	Co2+/Mg2+ efflux protein ApaG	-
BE963_RS13825	2177245..2178066	-	822	WP_001065361.1	16S rRNA (adenine(1518)-N(6)/adenine(1519)-N(6))- dimethyltransferase RsmA	-
BE963_RS13830	2178063..2179052	-	990	WP_000241280.1	4-hydroxythreonine-4-phosphate dehydrogenase PdxA	-
BE963_RS13835	2179052..2180338	-	1287	WP_000800470.1	peptidylprolyl isomerase SurA	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin CcdB (2-57)

Antitoxin

• Post-segregation antitoxin CcdA (8-77)

Sequences

Toxin

Protein

• Length: 63 a.a.
• Molecular weight: 7029.22 Da
• Isoelectric Point: 8.7938

>T71053 WP_001586653.1 NZ_CP018957:2175712-2175900 [Escherichia coli]
MQFTVYRSRGSNAAFPFVIDVTSDIIGEINRSIVIPLTPIERFIRIRPPERLNTILLLVDGS

Nucleotide

• Length: 189 bp

>T71053 NZ_CP018957:2175712-2175900 [Escherichia coli]
ATGCAATTTACGGTATACCGCAGTCGCGGCAGTAACGCCGCGTTTCCCTTTGTTATCGATGTTACCAGCGACATTATTGG
TGAGATTAATCGCAGTATTGTTATTCCATTAACACCTATTGAACGATTTATCCGTATTCGCCCTCCAGAACGGCTTAACA
CAATCCTTTTACTGGTAGACGGCTCATGA

Antitoxin

Protein

• Length: 78 a.a.
• Molecular weight: 8530.53 Da
• Isoelectric Point: 5.8062

>AT71053 WP_000125566.1 NZ_CP018957:2175476-2175709 [Escherichia coli]
MTAKRTTQSVTVTVDRELVNRARDAGLNMSATLTVALNAELKKHAATRWREENAEAIAALNQLADETGCFSDEYRSF

Nucleotide

• Length: 234 bp

>AT71053 NZ_CP018957:2175476-2175709 [Escherichia coli]
ATGACTGCAAAACGTACCACACAAAGTGTGACCGTCACCGTCGACCGTGAGTTAGTCAATCGCGCTCGTGATGCAGGCTT
AAATATGAGCGCCACCCTTACGGTTGCGCTCAATGCTGAACTTAAAAAACATGCAGCAACACGTTGGCGTGAAGAGAACG
CAGAAGCTATCGCTGCGTTAAATCAATTGGCTGATGAAACCGGATGTTTCTCTGATGAGTACCGGAGCTTCTAG

Structures

Antitoxin

• AlphaFold DB: A0A7U9LTM9