Detailed information of TA system TA071044

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 476130..476964
• Replicon: chromosome
• Accession: NZ_CP018957
• Organism: Escherichia coli strain Ecol_316

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: A0A8E0IX31
• Locus tag: BE963_RS05215
• Protein ID: WP_000854689.1
• Coordinates: 476587..476964 (+)
• Length: 126 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: A0A8E0IVP8
• Locus tag: BE963_RS05210
• Protein ID: WP_001285599.1
• Coordinates: 476130..476510 (+)
• Length: 127 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BE963_RS05170	472217..472672	+	456	WP_001309747.1	hypothetical protein	-
BE963_RS28525	472773..472904	+	132	WP_106108514.1	DUF905 family protein	-
BE963_RS05185	473083..473904	+	822	WP_001234555.1	DUF945 domain-containing protein	-
BE963_RS05190	474168..474641	+	474	WP_001538139.1	antirestriction protein	-
BE963_RS05195	474657..475133	+	477	WP_001186761.1	RadC family protein	-
BE963_RS05200	475196..475417	+	222	WP_000692315.1	DUF987 domain-containing protein	-
BE963_RS05205	475436..476080	+	645	WP_000086757.1	hypothetical protein	-
BE963_RS05210	476130..476510	+	381	WP_001285599.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
BE963_RS05215	476587..476964	+	378	WP_000854689.1	TA system toxin CbtA family protein	Toxin
BE963_RS05220	476961..477449	+	489	WP_000761698.1	hypothetical protein	-
BE963_RS05225	477466..477663	+	198	WP_000839250.1	DUF957 domain-containing protein	-
BE963_RS05230	477748..478593	+	846	WP_001446750.1	DUF4942 domain-containing protein	-
BE963_RS05235	478662..479057	+	396	WP_000208381.1	type IV toxin-antitoxin system AbiEi family antitoxin domain-containing protein	-
BE963_RS05240	479050..479983	+	934	Protein_466	nucleotidyl transferase AbiEii/AbiGii toxin family protein	-
BE963_RS29125	480415..480570	+	156	WP_000729638.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	flank	IS/Tn	-	-	480415..480570	155

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (18-117)

Sequences

Toxin

Protein

• Length: 126 a.a.
• Molecular weight: 14042.07 Da
• Isoelectric Point: 9.1510

>T71044 WP_000854689.1 NZ_CP018957:476587-476964 [Escherichia coli]
MKTLPDTHVRAASRCPSPVTIWQTLLTRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
SAGAPSQLINSIDILRARRATGLMTRNNYRMVNNITQGKHPEAKR

Nucleotide

• Length: 378 bp

>T71044 NZ_CP018957:476587-476964 [Escherichia coli]
ATGAAAACATTACCCGACACACACGTACGGGCGGCATCGCGCTGCCCGTCTCCCGTCACCATCTGGCAGACACTGCTCAC
CCGACTGCTGGACCAGCACTATGGCCTCACGCTGAATGACACACCGTTCGCTGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGGATTTCACTGTGTGACGCGGTGAACTTTCTCGTTGAAAAATACGCGCTGGTACGTACCGACCAGCCGGGATTC
AGCGCAGGAGCCCCGTCGCAGTTAATCAACAGCATTGATATTCTCCGGGCTCGCAGGGCGACCGGCCTGATGACCCGGAA
CAATTACAGAATGGTAAATAACATTACCCAGGGCAAGCATCCGGAGGCAAAACGATGA

Antitoxin

Protein

• Length: 127 a.a.
• Molecular weight: 13984.78 Da
• Isoelectric Point: 5.0823

>AT71044 WP_001285599.1 NZ_CP018957:476130-476510 [Escherichia coli]
MSDTLPGTTLPDDNKDRPWWGLPCTVTPCFGARLLQEGNRLHYLADRAGIRGRFSNADAYHLDQAFPLLMKQLELMLTSG
ELNPRHQHTVTLYAKGLTCDADTLGSCGYVYLAVYPATETESNPPE

Nucleotide

• Length: 381 bp

>AT71044 NZ_CP018957:476130-476510 [Escherichia coli]
ATGTCGGACACACTCCCCGGAACAACGCTTCCCGACGACAACAAAGACCGCCCCTGGTGGGGGCTGCCCTGCACCGTGAC
GCCCTGTTTCGGCGCCCGTCTGTTGCAGGAGGGTAACCGGTTGCATTACCTTGCAGACCGCGCCGGTATCAGGGGCCGGT
TCAGCAATGCGGATGCATACCATCTGGACCAGGCTTTTCCACTGCTGATGAAACAACTGGAACTCATGCTCACCAGCGGT
GAACTGAATCCCCGCCATCAGCATACCGTCACGCTGTATGCGAAAGGGCTGACCTGCGATGCCGACACCCTCGGGTCCTG
TGGTTACGTTTATCTGGCTGTTTATCCTGCGACCGAAACGGAAAGTAACCCTCCGGAATAG