Detailed information of TA system TA071041

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vagCD/VapC-VagC
• Location: 77428..78071
• Replicon: plasmid pEC316_2
• Accession: NZ_CP018955
• Organism: Escherichia coli strain Ecol_316

Toxin (Protein)

• Gene name: vagD
• Uniprot ID: -
• Locus tag: BE963_RS01205
• Protein ID: WP_078174841.1
• Coordinates: 77655..78071 (+)
• Length: 139 a.a.

Antitoxin (Protein)

• Gene name: vagC
• Uniprot ID: -
• Locus tag: BE963_RS01200
• Protein ID: WP_078174839.1
• Coordinates: 77428..77658 (+)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BE963_RS01160	72434..72907	+	474	WP_047353183.1	YkgJ family cysteine cluster protein	-
BE963_RS01165	73064..74026	-	963	WP_004197772.1	Abi family protein	-
BE963_RS01170	74193..74975	-	783	WP_004197768.1	site-specific integrase	-
BE963_RS01175	74975..75793	-	819	WP_004197776.1	hypothetical protein	-
BE963_RS01180	75842..76171	-	330	WP_004197773.1	hypothetical protein	-
BE963_RS01185	76199..76471	-	273	WP_162138577.1	hypothetical protein	-
BE963_RS01190	76563..76883	-	321	WP_004197770.1	hypothetical protein	-
BE963_RS01195	77011..77471	-	461	Protein_97	hypothetical protein	-
BE963_RS01200	77428..77658	+	231	WP_078174839.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
BE963_RS01205	77655..78071	+	417	WP_078174841.1	type II toxin-antitoxin system VapC family toxin	Toxin
BE963_RS01210	78145..78381	+	237	WP_001348075.1	AAA family ATPase	-
BE963_RS01215	78428..79132	+	705	WP_001067855.1	IS6-like element IS26 family transposase	-
BE963_RS28410	79365..79526	+	162	WP_001326844.1	hypothetical protein	-
BE963_RS01220	79523..80134	-	612	WP_000176305.1	DUF2913 family protein	-
BE963_RS01225	80188..80469	-	282	WP_001568067.1	hypothetical protein	-
BE963_RS01230	80642..80977	+	336	WP_011977766.1	C2H2-type zinc finger protein	-
BE963_RS01235	81965..82978	-	1014	WP_063267105.1	replication initiation protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..101186	101186
	-	flank	IS/Tn	-	-	78428..79132	704

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (6-129)

Antitoxin

• SpoVT-AbrB domain (7-47)

Sequences

Toxin

Protein

• Length: 139 a.a.
• Molecular weight: 15186.65 Da
• Isoelectric Point: 7.8638

>T71041 WP_078174841.1 NZ_CP018955:77655-78071 [Escherichia coli]
MKKTWMLDTNICSFIMREQPEAVLKRLEQAVLRGQRIVVSAITYSEMRFGATGPKASPRHVELVDAFCARLDAILPWDRA
AVDATTEIKVALRMAGTPIGPNDTAIAGHAIAAGAVLVTNNTREFERVPGLVLEDWVR

Nucleotide

• Length: 417 bp

>T71041 NZ_CP018955:77655-78071 [Escherichia coli]
ATGAAGAAAACCTGGATGCTCGACACCAACATCTGCTCGTTCATCATGCGCGAGCAGCCGGAAGCCGTCCTGAAGCGCCT
GGAGCAGGCGGTGCTGCGCGGCCAGCGCATCGTTGTCTCGGCCATCACTTATTCTGAAATGCGCTTCGGGGCCACCGGCC
CGAAGGCCTCCCCGCGCCACGTCGAGCTGGTCGACGCGTTCTGCGCCCGCCTCGATGCCATCCTGCCCTGGGATCGCGCT
GCGGTGGACGCCACCACGGAGATTAAGGTGGCGCTGCGAATGGCCGGGACGCCGATCGGCCCGAACGACACGGCCATTGC
CGGGCACGCCATCGCCGCCGGTGCCGTGCTGGTGACGAATAATACGCGGGAATTTGAGCGGGTACCGGGTCTTGTGCTGG
AAGACTGGGTAAGATAA

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8755.80 Da
• Isoelectric Point: 4.3775

>AT71041 WP_078174839.1 NZ_CP018955:77428-77658 [Escherichia coli]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDSIILRPVRPTWGSFAQFEKADPDFMAEREDVVSDEGRFEP

Nucleotide

• Length: 231 bp

>AT71041 NZ_CP018955:77428-77658 [Escherichia coli]
ATGAGAACTGTATCCATTTTTAAAAACGGCAATAACCGCGCCATTCGTCTGCCCCGCGATCTGGATTTTGAGGGAGTGAG
CGAGCTGGAGATCGTCCGGGAAGGGGACAGCATTATTTTGCGTCCCGTCCGGCCGACCTGGGGCTCGTTCGCGCAGTTCG
AGAAGGCAGACCCGGACTTTATGGCGGAGCGCGAGGACGTTGTCAGCGATGAAGGACGCTTTGAGCCATGA