Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) relBE/RHH(antitoxin)
Location 48522..49081 Replicon plasmid pEC316_3
Accession NZ_CP018954
Organism Escherichia coli strain Ecol_316

Toxin (Protein)


Gene name relE Uniprot ID V0SVC8
Locus tag BE963_RS00300 Protein ID WP_000421272.1
Coordinates 48522..48797 (-) Length 92 a.a.

Antitoxin (Protein)


Gene name relB Uniprot ID V0SRR3
Locus tag BE963_RS00305 Protein ID WP_001387467.1
Coordinates 48797..49081 (-) Length 95 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
BE963_RS00265 44572..45312 - 741 WP_001586233.1 tyrosine-type recombinase/integrase -
BE963_RS28340 45433..45615 - 183 WP_042004909.1 hypothetical protein -
BE963_RS00280 45958..46265 - 308 Protein_49 type II toxin-antitoxin system ParD family antitoxin -
BE963_RS00290 46758..47927 + 1170 WP_001072355.1 hypothetical protein -
BE963_RS28345 47960..48088 + 129 Protein_51 IS481 family transposase -
BE963_RS00295 48123..48416 - 294 WP_001105060.1 hypothetical protein -
BE963_RS00300 48522..48797 - 276 WP_000421272.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
BE963_RS00305 48797..49081 - 285 WP_001387467.1 ribbon-helix-helix domain-containing protein Antitoxin
BE963_RS00310 49686..50438 + 753 WP_000562172.1 IclR family transcriptional regulator -
BE963_RS00315 50484..51449 - 966 WP_001022265.1 2-keto-3-deoxygluconate transporter -
BE963_RS00320 51482..51862 - 381 WP_000710783.1 Rid family detoxifying hydrolase -
BE963_RS00325 51887..52777 - 891 WP_001077068.1 dihydrodipicolinate synthase family protein -
BE963_RS00330 53010..53204 - 195 WP_000796505.1 hypothetical protein -
BE963_RS00335 53178..53618 + 441 Protein_60 zinc-binding dehydrogenase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid mph(A) / sul1 / qacE / aadA5 / aph(6)-Id / aph(3'')-Ib / sul2 / blaTEM-1B - 1..97654 97654


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(3-90)

Antitoxin

Ribbon-helix-helix protein, CopG

(14-51)


Sequences

Toxin        


Download         Length: 92 a.a.        Molecular weight: 10749.45 Da        Isoelectric Point: 10.0871

>T71040 WP_000421272.1 NZ_CP018954:c48797-48522 [Escherichia coli]
MELKWTSKALSDLSRLFDFLVLASKPAAARTVQSLTQAPVILLTHPRMGEQLFQFEPREVRRIFAGEYEIRYEINGQTIY
VLRLWHTRENR

Download         Length: 276 bp

>T71040 NZ_CP018954:c48797-48522 [Escherichia coli]
ATGGAACTGAAGTGGACCAGTAAGGCGCTTTCTGATTTGTCGCGGTTATTTGATTTTCTGGTGCTGGCCAGTAAACCTGC
TGCCGCCAGAACGGTACAGTCCCTGACACAGGCACCGGTCATTCTGTTAACTCATCCACGTATGGGAGAACAGTTGTTTC
AGTTTGAACCCAGGGAGGTCAGACGGATTTTTGCTGGCGAGTACGAAATCCGTTACGAAATTAATGGCCAGACTATTTAT
GTATTGCGTCTGTGGCACACACGAGAAAACAGGTAG

Antitoxin


Download         Length: 95 a.a.        Molecular weight: 10460.87 Da        Isoelectric Point: 5.6558

>AT71040 WP_001387467.1 NZ_CP018954:c49081-48797 [Escherichia coli]
MQMKNNAAQATKVITAHVPLPMADKVDQMAARLERSRGWVIKQALSAWLAQEEERNRLTLEALDDVTSGQVIDHQAVQAW
SDSLSTDNPLPVPR

Download         Length: 285 bp

>AT71040 NZ_CP018954:c49081-48797 [Escherichia coli]
ATGCAAATGAAAAACAATGCCGCACAAGCAACAAAAGTAATTACCGCGCATGTGCCATTACCTATGGCTGATAAAGTCGA
CCAGATGGCCGCCAGACTGGAACGTTCCCGGGGCTGGGTTATCAAACAGGCGCTTTCTGCATGGCTTGCCCAGGAGGAGG
AGCGTAATCGCCTGACGCTGGAAGCCCTGGACGATGTGACATCCGGACAGGTTATCGACCATCAGGCTGTACAGGCCTGG
TCGGACAGCCTCAGTACTGACAATCCGTTACCGGTGCCACGCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB V0SVC8

Antitoxin

Source ID Structure
AlphaFold DB A0A0E0Y880

References