Detailed information of TA system TA071039

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): kacAT/ElaA-DUF1778
• Location: 41569..42372
• Replicon: plasmid pEC316_3
• Accession: NZ_CP018954
• Organism: Escherichia coli strain Ecol_316

Toxin (Protein)

• Gene name: KacT
• Uniprot ID: G3CAI8
• Locus tag: BE963_RS00250
• Protein ID: WP_000348883.1
• Coordinates: 41569..42099 (-)
• Length: 177 a.a.

Antitoxin (Protein)

• Gene name: KacA
• Uniprot ID: G3CAI7
• Locus tag: BE963_RS00255
• Protein ID: WP_001275013.1
• Coordinates: 42103..42372 (-)
• Length: 90 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BE963_RS00235	37932..40553	+	2622	WP_001586230.1	hypothetical protein	-
BE963_RS00240	40752..40982	+	231	WP_001261274.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	-
BE963_RS00245	41071..41346	+	276	WP_001403920.1	type II toxin-antitoxin system VapC family toxin	-
BE963_RS28335	41351..41568	-	218	Protein_43	transposase	-
BE963_RS00250	41569..42099	-	531	WP_000348883.1	GNAT family N-acetyltransferase	Toxin
BE963_RS00255	42103..42372	-	270	WP_001275013.1	DUF1778 domain-containing protein	Antitoxin
BE963_RS00260	43260..44249	+	990	WP_123002471.1	RepB family plasmid replication initiator protein	-
BE963_RS00265	44572..45312	-	741	WP_001586233.1	tyrosine-type recombinase/integrase	-
BE963_RS28340	45433..45615	-	183	WP_042004909.1	hypothetical protein	-
BE963_RS00280	45958..46265	-	308	Protein_49	type II toxin-antitoxin system ParD family antitoxin	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	mph(A) / sul1 / qacE / aadA5 / aph(6)-Id / aph(3'')-Ib / sul2 / blaTEM-1B	-	1..97654	97654

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (92-156)

Antitoxin

• Vibrio phage ICP1, Orf50 (8-86)

Sequences

Toxin

Protein

• Length: 177 a.a.
• Molecular weight: 20321.20 Da
• Isoelectric Point: 6.4889

>T71039 WP_000348883.1 NZ_CP018954:c42099-41569 [Escherichia coli]
MDGLRIEIFSEEVEYQLSNFDCGEEYLNTFLTDHLQRQHSSKILRGYLLVTREDRPRVMGYYTLSGSCFEKILLPSKTQQ
KRVPYKNVPSVTLGRLAIDKSIHHQGYGETLVTHAMKVVYQASQAVGIHGMFVEALNDNAKKFYLRLGFIQLKEENCDSL
FYPTKSIEVLFEVNDE

Nucleotide

• Length: 531 bp

>T71039 NZ_CP018954:c42099-41569 [Escherichia coli]
ATGGACGGTTTAAGGATTGAGATTTTTTCAGAGGAAGTTGAATATCAATTAAGCAACTTCGATTGTGGAGAAGAGTATCT
GAATACTTTTCTCACCGATCATCTGCAACGTCAGCACAGTAGTAAGATTTTACGGGGTTATCTGCTCGTGACGAGAGAGG
ATAGACCACGTGTCATGGGGTACTACACCCTGTCAGGCAGTTGCTTTGAGAAAATACTTCTCCCCAGCAAAACACAACAA
AAGCGTGTGCCCTACAAAAATGTGCCGAGCGTGACTTTGGGCCGTCTGGCAATTGATAAAAGCATTCATCACCAGGGATA
TGGAGAAACACTTGTTACCCATGCGATGAAAGTCGTGTATCAGGCCTCGCAGGCTGTGGGTATACACGGAATGTTCGTGG
AGGCGCTGAACGATAACGCCAAAAAATTCTACCTTCGACTGGGTTTTATACAGTTAAAGGAAGAAAACTGTGATTCGCTC
TTCTACCCGACAAAATCTATTGAAGTATTGTTCGAAGTAAATGACGAATAA

Antitoxin

Protein

• Length: 90 a.a.
• Molecular weight: 10229.63 Da
• Isoelectric Point: 6.4702

>AT71039 WP_001275013.1 NZ_CP018954:c42372-42103 [Escherichia coli]
MSALKKQRIDLRLNEDDKHMIEEAAAMTNQSISQFMVSTASARAAEVIDQHRRLLLNEESWNLVMDAITNPPAPNDRLKR
ATNRLRELE

Nucleotide

• Length: 270 bp

>AT71039 NZ_CP018954:c42372-42103 [Escherichia coli]
ATGTCAGCACTGAAGAAGCAGCGCATCGATCTGAGATTAAATGAAGACGACAAACACATGATCGAAGAAGCTGCAGCAAT
GACTAACCAGTCCATTTCGCAGTTCATGGTCAGTACCGCCTCAGCACGTGCTGCTGAAGTTATTGATCAGCACCGTCGCC
TGCTCCTCAATGAGGAGTCCTGGAATCTGGTTATGGATGCAATCACTAATCCGCCAGCACCAAATGACCGGCTGAAACGC
GCCACTAACCGTCTCCGGGAACTGGAGTAA

Structures

Toxin

• AlphaFold DB: G3CAI8

Antitoxin

• AlphaFold DB: G3CAI7