Detailed information of TA system TA071038

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vagCD/VapC-VagC
• Location: 40752..41346
• Replicon: plasmid pEC316_3
• Accession: NZ_CP018954
• Organism: Escherichia coli strain Ecol_316

Toxin (Protein)

• Gene name: vagD
• Uniprot ID: -
• Locus tag: BE963_RS00245
• Protein ID: WP_001403920.1
• Coordinates: 41071..41346 (+)
• Length: 92 a.a.

Antitoxin (Protein)

• Gene name: vagC
• Uniprot ID: G3CAJ1
• Locus tag: BE963_RS00240
• Protein ID: WP_001261274.1
• Coordinates: 40752..40982 (+)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BE963_RS00230	35879..37378	-	1500	WP_000174402.1	IS21-like element ISEc10 family transposase	-
BE963_RS00235	37932..40553	+	2622	WP_001586230.1	hypothetical protein	-
BE963_RS00240	40752..40982	+	231	WP_001261274.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
BE963_RS00245	41071..41346	+	276	WP_001403920.1	type II toxin-antitoxin system VapC family toxin	Toxin
BE963_RS28335	41351..41568	-	218	Protein_43	transposase	-
BE963_RS00250	41569..42099	-	531	WP_000348883.1	GNAT family N-acetyltransferase	-
BE963_RS00255	42103..42372	-	270	WP_001275013.1	DUF1778 domain-containing protein	-
BE963_RS00260	43260..44249	+	990	WP_123002471.1	RepB family plasmid replication initiator protein	-
BE963_RS00265	44572..45312	-	741	WP_001586233.1	tyrosine-type recombinase/integrase	-
BE963_RS28340	45433..45615	-	183	WP_042004909.1	hypothetical protein	-
BE963_RS00280	45958..46265	-	308	Protein_49	type II toxin-antitoxin system ParD family antitoxin	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	mph(A) / sul1 / qacE / aadA5 / aph(6)-Id / aph(3'')-Ib / sul2 / blaTEM-1B	-	1..97654	97654

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• SpoVT-AbrB domain (7-45)

Sequences

Toxin

Protein

• Length: 92 a.a.
• Molecular weight: 9621.03 Da
• Isoelectric Point: 8.5444

>T71038 WP_001403920.1 NZ_CP018954:41071-41346 [Escherichia coli]
VFSHRIVVSAITYSEMRFGASGPKASPRHVQLVDKFCPCLDAILSWDRAAVDGTTDIKVALRQAGTPIGQNDTAIAGPEA
GRLGKLTSPTL

Nucleotide

• Length: 276 bp

>T71038 NZ_CP018954:41071-41346 [Escherichia coli]
GTGTTTAGTCACCGCATTGTGGTCTCGGCCATTACTTACTCTGAAATGCGCTTCGGTGCCTCCGGTCCGAAGGCCTCCCC
GCGTCACGTGCAACTGGTTGATAAATTCTGCCCGTGCCTCGATGCCATCCTGTCTTGGGACCGCGCCGCGGTGGACGGCA
CCACGGATATTAAAGTGGCACTGCGCCAGGCCGGGACGCCGATCGGCCAGAACGATACGGCGATTGCCGGGCCTGAAGCT
GGAAGACTGGGTAAATTAACATCGCCCACACTCTGA

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8856.95 Da
• Isoelectric Point: 4.6286

>AT71038 WP_001261274.1 NZ_CP018954:40752-40982 [Escherichia coli]
MRTVSIFKNGNNRAIHLPRDLDFERVRELEIVREGDSIILRPVQPNWGSFAQLEKADPDFMAERENVVSDEGRFDL

Nucleotide

• Length: 231 bp

>AT71038 NZ_CP018954:40752-40982 [Escherichia coli]
ATGAGAACTGTATCCATTTTTAAAAACGGCAATAACCGCGCCATCCATCTGCCCCGCGATTTGGATTTTGAGCGGGTGAG
AGAGCTGGAGATCGTCCGCGAAGGAGACAGCATCATCCTGCGTCCCGTCCAGCCGAACTGGGGCTCATTCGCGCAGCTCG
AAAAAGCCGATCCGGATTTTATGGCGGAGCGCGAGAATGTTGTCAGCGACGAAGGTCGATTTGACCTGTGA

Structures

Antitoxin

• AlphaFold DB: G3CAJ1