Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 24670..25195 Replicon plasmid pEC316_3
Accession NZ_CP018954
Organism Escherichia coli strain Ecol_316

Toxin (Protein)


Gene name ccdB Uniprot ID V0SSI5
Locus tag BE963_RS00160 Protein ID WP_001159868.1
Coordinates 24670..24975 (-) Length 102 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID S1PPD8
Locus tag BE963_RS00165 Protein ID WP_000813634.1
Coordinates 24977..25195 (-) Length 73 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
BE963_RS00145 20580..21746 - 1167 WP_000772446.1 plasmid-partitioning protein SopA -
BE963_RS00150 22334..23089 - 756 WP_000852146.1 replication initiation protein RepE -
BE963_RS00155 23863..24669 - 807 WP_000016982.1 site-specific integrase -
BE963_RS00160 24670..24975 - 306 WP_001159868.1 type II toxin-antitoxin system toxin CcdB Toxin
BE963_RS00165 24977..25195 - 219 WP_000813634.1 type II toxin-antitoxin system antitoxin CcdA Antitoxin
BE963_RS00170 25903..26898 + 996 WP_000246635.1 hypothetical protein -
BE963_RS00175 26902..27834 + 933 WP_000991831.1 hypothetical protein -
BE963_RS00180 28127..28312 + 186 WP_001586223.1 hypothetical protein -
BE963_RS00185 28313..29215 + 903 WP_024187432.1 hypothetical protein -
BE963_RS00190 29217..30086 + 870 WP_001586225.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid mph(A) / sul1 / qacE / aadA5 / aph(6)-Id / aph(3'')-Ib / sul2 / blaTEM-1B - 1..97654 97654


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-100)

Antitoxin

Post-segregation antitoxin CcdA

(2-72)


Sequences

Toxin        


Download         Length: 102 a.a.        Molecular weight: 11706.51 Da        Isoelectric Point: 6.4674

>T71037 WP_001159868.1 NZ_CP018954:c24975-24670 [Escherichia coli]
MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKVSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEV
ADLSHRENDIKNAINLMFWGI

Download         Length: 306 bp

>T71037 NZ_CP018954:c24975-24670 [Escherichia coli]
ATGCAGTTTAAGGTTTACACCTATAAAAGAGAGAGCCGTTATCGTCTGTTTGTGGATGTACAGAGTGATATTATTGACAC
GCCCGGGCGACGGATGGTGATCCCCCTGGCCAGTGCACGTCTGCTGTCAGATAAAGTCTCCCGTGAACTTTACCCGGTGG
TGCATATCGGGGATGAAAGCTGGCGCATGATGACCACCGATATGGCCAGTGTGCCGGTCTCCGTTATCGGGGAAGAAGTG
GCTGATCTCAGCCACCGCGAAAATGACATCAAAAACGCCATTAACCTGATGTTCTGGGGAATATAA

Antitoxin


Download         Length: 73 a.a.        Molecular weight: 8372.34 Da        Isoelectric Point: 4.7405

>AT71037 WP_000813634.1 NZ_CP018954:c25195-24977 [Escherichia coli]
MKQRITVTVDSDSYQLLKAYDVNISGLVSTTMQNEARRLRAERWKAENQEGMAEVARFIEMNGSFADENRDW

Download         Length: 219 bp

>AT71037 NZ_CP018954:c25195-24977 [Escherichia coli]
ATGAAGCAGCGTATTACAGTGACAGTTGACAGCGACAGCTATCAGTTGCTCAAGGCATATGATGTCAATATCTCCGGTCT
GGTAAGCACAACCATGCAGAATGAAGCCCGTCGTCTGCGTGCCGAACGCTGGAAAGCGGAAAATCAGGAAGGGATGGCTG
AGGTCGCCCGGTTTATTGAAATGAACGGCTCTTTTGCTGACGAGAACAGGGACTGGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4VUB
PDB 2VUB
PDB 7EPI
PDB 7EPG
PDB 3VUB
PDB 1X75
PDB 1VUB
PDB 7EPJ
PDB 3HPW
PDB 3G7Z

Antitoxin

Source ID Structure
PDB 3TCJ
PDB 2H3A
PDB 2ADN
PDB 2ADL
PDB 3HPW
PDB 2H3C
PDB 3G7Z
AlphaFold DB A0A829CQY2

References