Detailed information of TA system TA071026

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 3295674..3296509
• Replicon: chromosome
• Accession: NZ_CP018953
• Organism: Escherichia coli strain Ecol_276

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: A0A0J2AEA6
• Locus tag: BE964_RS18495
• Protein ID: WP_000854759.1
• Coordinates: 3296132..3296509 (+)
• Length: 126 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: S1NM52
• Locus tag: BE964_RS18490
• Protein ID: WP_001295723.1
• Coordinates: 3295674..3296042 (+)
• Length: 123 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BE964_RS18450	3292789..3292947	+	159	WP_001323397.1	DUF905 family protein	-
BE964_RS18460	3293102..3293920	+	819	WP_001234738.1	DUF945 domain-containing protein	-
BE964_RS18470	3294262..3294735	+	474	WP_001350782.1	antirestriction protein	-
BE964_RS18475	3294751..3295227	+	477	WP_001186775.1	RadC family protein	-
BE964_RS18480	3295290..3295511	+	222	WP_000692345.1	DUF987 domain-containing protein	-
BE964_RS18490	3295674..3296042	+	369	WP_001295723.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
BE964_RS18495	3296132..3296509	+	378	WP_000854759.1	TA system toxin CbtA family protein	Toxin
BE964_RS18500	3296506..3296994	+	489	WP_000761690.1	hypothetical protein	-
BE964_RS18505	3297011..3297187	+	177	WP_000839286.1	DUF957 domain-containing protein	-
BE964_RS27635	3297287..3297445	+	159	WP_001467148.1	hypothetical protein	-
BE964_RS27440	3297809..3297985	+	177	Protein_3100	helix-turn-helix domain-containing protein	-
BE964_RS27445	3298022..3298207	+	186	Protein_3101	transposase	-
BE964_RS18515	3298400..3299941	+	1542	WP_001298859.1	IS21-like element ISEc12 family transposase	-
BE964_RS18520	3299956..3300702	+	747	WP_001016257.1	IS21-like element ISEc12 family helper ATPase IstB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (18-117)

Sequences

Toxin

Protein

• Length: 126 a.a.
• Molecular weight: 14144.26 Da
• Isoelectric Point: 7.3249

>T71026 WP_000854759.1 NZ_CP018953:3296132-3296509 [Escherichia coli]
MKTLPDTHVREVSCCPSPVTIWQTLLTRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDLPGF
SACTHSQLINSIDILRARRATGLMIRDNYRTVNNITLGKHPEAKR

Nucleotide

• Length: 378 bp

>T71026 NZ_CP018953:3296132-3296509 [Escherichia coli]
ATGAAAACATTACCCGACACGCATGTACGGGAGGTATCGTGCTGCCCGTCTCCAGTCACCATCTGGCAGACACTGCTCAC
CCGACTGCTGGACCAGCACTACGGCCTTACGCTGAATGACACACCGTTCGCTGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGCATTTCACTGTGTGATGCAGTGAACTTTCTCGTGGAAAAATACGCACTGGTACGTACCGACCTGCCGGGATTC
AGCGCCTGTACCCACTCTCAGTTAATAAACAGCATCGATATCCTCCGGGCTCGCCGGGCAACAGGCCTGATGATCCGCGA
CAATTACAGAACGGTAAATAACATTACCCTGGGGAAGCATCCGGAGGCGAAACGATGA

Antitoxin

Protein

• Length: 123 a.a.
• Molecular weight: 13592.40 Da
• Isoelectric Point: 6.6255

>AT71026 WP_001295723.1 NZ_CP018953:3295674-3296042 [Escherichia coli]
VSDTLSGTTHPDDNDDHPWWGLPCTVTPCFGARLVQEGNRLHYLADRAGIRGRFRDADAYPLDQAFPLLMKQLKLMLTSG
ELNPRHQHTVTLYAKGLTCEADTLGSCGYVYLAVYPTPETKK

Nucleotide

• Length: 369 bp

>AT71026 NZ_CP018953:3295674-3296042 [Escherichia coli]
GTGTCAGACACACTTTCCGGGACAACGCATCCCGATGACAATGACGACCATCCCTGGTGGGGACTACCCTGCACAGTGAC
GCCCTGTTTCGGCGCCCGTCTGGTGCAGGAGGGTAACCGGTTGCATTACCTTGCCGACCGTGCCGGTATCAGAGGCCGGT
TCAGAGATGCGGATGCGTACCCTCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGAAATTAATGCTCACCAGTGGT
GAACTGAATCCCCGCCATCAGCATACCGTGACACTGTATGCGAAGGGGCTGACCTGTGAAGCCGACACCCTCGGCTCCTG
TGGTTACGTTTATCTGGCTGTTTATCCGACACCTGAAACGAAAAAGTAA

Structures

Toxin

• AlphaFold DB: A0A0J2AEA6

Antitoxin

• AlphaFold DB: S1NM52