Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) phd-doc/Doc-Phd
Location 41772..42373 Replicon plasmid pEC276_1
Accession NZ_CP018952
Organism Escherichia coli strain Ecol_276

Toxin (Protein)


Gene name doc Uniprot ID V0AJ64
Locus tag BE964_RS00990 Protein ID WP_001216034.1
Coordinates 41772..42152 (-) Length 127 a.a.

Antitoxin (Protein)


Gene name phd Uniprot ID U9YQH9
Locus tag BE964_RS00995 Protein ID WP_001190712.1
Coordinates 42152..42373 (-) Length 74 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
BE964_RS00955 36823..37755 - 933 WP_000081352.1 homocysteine S-methyltransferase -
BE964_RS00960 37742..39145 - 1404 WP_001373486.1 S-methylmethionine permease -
BE964_RS00965 39389..40369 + 981 WP_000019407.1 IS5-like element IS5 family transposase -
BE964_RS00970 40597..40914 + 318 WP_001513659.1 hypothetical protein -
BE964_RS00975 40864..41277 - 414 Protein_46 DDE-type integrase/transposase/recombinase -
BE964_RS00980 41201..41560 - 360 WP_001513660.1 hypothetical protein -
BE964_RS00985 41588..41767 - 180 WP_001513661.1 hypothetical protein -
BE964_RS00990 41772..42152 - 381 WP_001216034.1 type II toxin-antitoxin system death-on-curing family toxin Toxin
BE964_RS00995 42152..42373 - 222 WP_001190712.1 type II toxin-antitoxin system Phd/YefM family antitoxin Antitoxin
BE964_RS01000 42556..44112 + 1557 WP_001617892.1 type I restriction-modification system subunit M -
BE964_RS01005 44109..45380 + 1272 WP_023142242.1 restriction endonuclease subunit S -
BE964_RS01010 45502..46737 + 1236 Protein_53 HsdR family type I site-specific deoxyribonuclease -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid - senB 1..132345 132345
- flank IS/Tn - - 46987..47490 503


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Death on curing protein

(2-123)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-51)


Sequences

Toxin        


Download         Length: 127 a.a.        Molecular weight: 13615.32 Da        Isoelectric Point: 5.1514

>T70998 WP_001216034.1 NZ_CP018952:c42152-41772 [Escherichia coli]
MRHISPEELIALHDANINRYGGLPGMSDPGRAEAIIGRVQARVAYEEITDLFEVSATYLVATARGHIFNDANKRTALNSA
LLFLRRNGVQVFDSPELADLTVGAATGEISVSSVADTLRRLYGSAE

Download         Length: 381 bp

>T70998 NZ_CP018952:c42152-41772 [Escherichia coli]
ATGAGGCATATATCACCGGAAGAACTTATTGCGCTTCATGATGCGAATATAAACCGCTACGGCGGCCTGCCGGGAATGTC
TGATCCGGGCAGGGCAGAGGCCATTATCGGGAGAGTTCAGGCCAGAGTTGCCTACGAAGAGATCACCGACCTTTTCGAAG
TCTCCGCCACCTACCTGGTGGCTACAGCGAGAGGGCATATATTCAATGATGCCAATAAGCGTACCGCGCTAAACAGTGCG
CTGTTATTTCTACGCCGTAACGGGGTGCAGGTATTTGATTCACCTGAACTGGCAGACCTTACCGTAGGGGCTGCGACCGG
AGAGATATCTGTATCTTCTGTCGCCGACACGTTACGTAGATTGTATGGTTCTGCGGAGTAG

Antitoxin


Download         Length: 74 a.a.        Molecular weight: 8133.10 Da        Isoelectric Point: 4.7708

>AT70998 WP_001190712.1 NZ_CP018952:c42373-42152 [Escherichia coli]
MQSINFRTARGNLSEVLNNVEAGEEVEITRRGREPAVIVSKATFEAYKKAALDAEFASLFDTLDSTNKELVNR

Download         Length: 222 bp

>AT70998 NZ_CP018952:c42373-42152 [Escherichia coli]
ATGCAATCCATTAACTTCCGTACCGCGCGCGGCAACCTTTCTGAAGTGCTCAACAATGTTGAGGCCGGGGAAGAGGTTGA
AATCACCCGCAGAGGCCGTGAGCCAGCAGTAATTGTCAGCAAGGCTACTTTCGAAGCCTACAAAAAAGCGGCGCTGGATG
CTGAATTTGCATCCCTGTTTGACACCCTGGACTCCACCAACAAGGAACTGGTTAACCGATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB V0AJ64

Antitoxin

Source ID Structure
AlphaFold DB A0A829CJB6

References