Detailed information of TA system
Bioinformatically predictedOverview
TA module
Type | II | Classification (family/domain) | hicAB/- |
Location | 1969..2561 | Replicon | plasmid pEC276_3 |
Accession | NZ_CP018950 | ||
Organism | Escherichia coli strain Ecol_276 |
Toxin (Protein)
Gene name | hicA | Uniprot ID | V0AGQ2 |
Locus tag | BE964_RS00135 | Protein ID | WP_000520549.1 |
Coordinates | 1969..2217 (+) | Length | 83 a.a. |
Antitoxin (Protein)
Gene name | hicB | Uniprot ID | V0AWG0 |
Locus tag | BE964_RS00140 | Protein ID | WP_000681613.1 |
Coordinates | 2214..2561 (+) | Length | 116 a.a. |
Genomic Context
Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
---|---|---|---|---|---|---|
BE964_RS00110 | 1..252 | + | 252 | WP_001230707.1 | hypothetical protein | - |
BE964_RS00115 | 239..466 | + | 228 | WP_000801440.1 | hypothetical protein | - |
BE964_RS00125 | 920..1219 | + | 300 | WP_000650310.1 | hypothetical protein | - |
BE964_RS00130 | 1314..1664 | + | 351 | WP_000854260.1 | hypothetical protein | - |
BE964_RS00135 | 1969..2217 | + | 249 | WP_000520549.1 | type II toxin-antitoxin system HicA family toxin | Toxin |
BE964_RS00140 | 2214..2561 | + | 348 | WP_000681613.1 | type II toxin-antitoxin system HicB family antitoxin | Antitoxin |
BE964_RS00150 | 2925..3428 | + | 504 | WP_000781817.1 | hypothetical protein | - |
BE964_RS00155 | 3432..4661 | + | 1230 | WP_001329504.1 | relaxase/mobilization nuclease domain-containing protein | - |
BE964_RS00160 | 4738..5004 | + | 267 | WP_000160396.1 | hypothetical protein | - |
BE964_RS00165 | 5009..5242 | + | 234 | WP_000121161.1 | EexN family lipoprotein | - |
BE964_RS00170 | 5598..6218 | + | 621 | WP_000157548.1 | lytic transglycosylase domain-containing protein | - |
BE964_RS00175 | 6286..6558 | + | 273 | WP_001328551.1 | TrbC/VirB2 family protein | - |
Associated MGEs
MGE detail |
Similar MGEs |
Relative position |
MGE Type | Cargo ARG | Virulence gene | Coordinates | Length (bp) |
---|---|---|---|---|---|---|---|
- | inside | Non-Mobilizable plasmid | - | - | 1..35225 | 35225 |
Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.
Sequences
Toxin
Download Length: 83 a.a. Molecular weight: 9422.08 Da Isoelectric Point: 10.4800
>T70997 WP_000520549.1 NZ_CP018950:1969-2217 [Escherichia coli]
MGKTDKLLAKFLNSKKTFEWDELVVLFSSLGYVKKEMQGSRVRFFNAEINHTILMHRPHPESYIKGGTLKAIKQNLKEAG
LL
MGKTDKLLAKFLNSKKTFEWDELVVLFSSLGYVKKEMQGSRVRFFNAEINHTILMHRPHPESYIKGGTLKAIKQNLKEAG
LL
Download Length: 249 bp
>T70997 NZ_CP018950:1969-2217 [Escherichia coli]
ATGGGGAAAACAGATAAGCTACTGGCAAAGTTTTTAAACAGTAAAAAAACGTTTGAATGGGATGAACTGGTCGTTTTGTT
TTCCTCTCTGGGGTATGTCAAAAAGGAAATGCAGGGGTCAAGAGTGCGGTTTTTCAATGCTGAAATCAATCACACCATAT
TAATGCATCGTCCACATCCGGAAAGTTATATCAAAGGTGGTACGCTGAAAGCAATTAAACAGAATCTGAAAGAGGCTGGA
TTATTATGA
ATGGGGAAAACAGATAAGCTACTGGCAAAGTTTTTAAACAGTAAAAAAACGTTTGAATGGGATGAACTGGTCGTTTTGTT
TTCCTCTCTGGGGTATGTCAAAAAGGAAATGCAGGGGTCAAGAGTGCGGTTTTTCAATGCTGAAATCAATCACACCATAT
TAATGCATCGTCCACATCCGGAAAGTTATATCAAAGGTGGTACGCTGAAAGCAATTAAACAGAATCTGAAAGAGGCTGGA
TTATTATGA
Antitoxin
Download Length: 116 a.a. Molecular weight: 13113.84 Da Isoelectric Point: 4.6723
>AT70997 WP_000681613.1 NZ_CP018950:2214-2561 [Escherichia coli]
MKHLKYKGYLGTVEPDFENNVLYGKLAFIRDLVTYEASTLAELEQEFKTSVDLYLQSCVEDGKEPDTPFKGVFNVRLDPE
LHRRVAEMAMEEDLSLNAFVNKALEKEVSNHRAGA
MKHLKYKGYLGTVEPDFENNVLYGKLAFIRDLVTYEASTLAELEQEFKTSVDLYLQSCVEDGKEPDTPFKGVFNVRLDPE
LHRRVAEMAMEEDLSLNAFVNKALEKEVSNHRAGA
Download Length: 348 bp
>AT70997 NZ_CP018950:2214-2561 [Escherichia coli]
ATGAAACATTTAAAATATAAAGGATATTTAGGCACGGTTGAACCCGATTTTGAAAATAATGTCCTGTATGGAAAACTGGC
GTTTATTCGGGACCTGGTAACTTATGAGGCTTCTACATTAGCTGAACTGGAACAGGAGTTTAAAACATCTGTTGATCTGT
ATTTACAGTCTTGTGTGGAGGATGGAAAGGAACCTGATACCCCGTTTAAAGGCGTGTTTAACGTCAGGCTTGATCCAGAA
CTGCATCGTCGGGTAGCAGAAATGGCGATGGAAGAAGATTTATCGTTGAATGCCTTTGTTAATAAGGCTCTGGAAAAAGA
AGTCAGCAATCATCGCGCAGGGGCTTAA
ATGAAACATTTAAAATATAAAGGATATTTAGGCACGGTTGAACCCGATTTTGAAAATAATGTCCTGTATGGAAAACTGGC
GTTTATTCGGGACCTGGTAACTTATGAGGCTTCTACATTAGCTGAACTGGAACAGGAGTTTAAAACATCTGTTGATCTGT
ATTTACAGTCTTGTGTGGAGGATGGAAAGGAACCTGATACCCCGTTTAAAGGCGTGTTTAACGTCAGGCTTGATCCAGAA
CTGCATCGTCGGGTAGCAGAAATGGCGATGGAAGAAGATTTATCGTTGAATGCCTTTGTTAATAAGGCTCTGGAAAAAGA
AGTCAGCAATCATCGCGCAGGGGCTTAA
Similar Proteins
Only experimentally validated proteins are listed.
Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
---|
Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
---|
Structures
Toxin
Source | ID | Structure |
---|---|---|
AlphaFold DB | A0A829CIX3 |
Antitoxin
Source | ID | Structure |
---|---|---|
AlphaFold DB | A0A829CLC1 |