Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/-
Location 1969..2561 Replicon plasmid pEC276_3
Accession NZ_CP018950
Organism Escherichia coli strain Ecol_276

Toxin (Protein)


Gene name hicA Uniprot ID V0AGQ2
Locus tag BE964_RS00135 Protein ID WP_000520549.1
Coordinates 1969..2217 (+) Length 83 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID V0AWG0
Locus tag BE964_RS00140 Protein ID WP_000681613.1
Coordinates 2214..2561 (+) Length 116 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
BE964_RS00110 1..252 + 252 WP_001230707.1 hypothetical protein -
BE964_RS00115 239..466 + 228 WP_000801440.1 hypothetical protein -
BE964_RS00125 920..1219 + 300 WP_000650310.1 hypothetical protein -
BE964_RS00130 1314..1664 + 351 WP_000854260.1 hypothetical protein -
BE964_RS00135 1969..2217 + 249 WP_000520549.1 type II toxin-antitoxin system HicA family toxin Toxin
BE964_RS00140 2214..2561 + 348 WP_000681613.1 type II toxin-antitoxin system HicB family antitoxin Antitoxin
BE964_RS00150 2925..3428 + 504 WP_000781817.1 hypothetical protein -
BE964_RS00155 3432..4661 + 1230 WP_001329504.1 relaxase/mobilization nuclease domain-containing protein -
BE964_RS00160 4738..5004 + 267 WP_000160396.1 hypothetical protein -
BE964_RS00165 5009..5242 + 234 WP_000121161.1 EexN family lipoprotein -
BE964_RS00170 5598..6218 + 621 WP_000157548.1 lytic transglycosylase domain-containing protein -
BE964_RS00175 6286..6558 + 273 WP_001328551.1 TrbC/VirB2 family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - - 1..35225 35225


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(20-81)

Antitoxin

Uncharacterised protein family HicB

(54-104)


Sequences

Toxin        


Download         Length: 83 a.a.        Molecular weight: 9422.08 Da        Isoelectric Point: 10.4800

>T70997 WP_000520549.1 NZ_CP018950:1969-2217 [Escherichia coli]
MGKTDKLLAKFLNSKKTFEWDELVVLFSSLGYVKKEMQGSRVRFFNAEINHTILMHRPHPESYIKGGTLKAIKQNLKEAG
LL

Download         Length: 249 bp

>T70997 NZ_CP018950:1969-2217 [Escherichia coli]
ATGGGGAAAACAGATAAGCTACTGGCAAAGTTTTTAAACAGTAAAAAAACGTTTGAATGGGATGAACTGGTCGTTTTGTT
TTCCTCTCTGGGGTATGTCAAAAAGGAAATGCAGGGGTCAAGAGTGCGGTTTTTCAATGCTGAAATCAATCACACCATAT
TAATGCATCGTCCACATCCGGAAAGTTATATCAAAGGTGGTACGCTGAAAGCAATTAAACAGAATCTGAAAGAGGCTGGA
TTATTATGA

Antitoxin


Download         Length: 116 a.a.        Molecular weight: 13113.84 Da        Isoelectric Point: 4.6723

>AT70997 WP_000681613.1 NZ_CP018950:2214-2561 [Escherichia coli]
MKHLKYKGYLGTVEPDFENNVLYGKLAFIRDLVTYEASTLAELEQEFKTSVDLYLQSCVEDGKEPDTPFKGVFNVRLDPE
LHRRVAEMAMEEDLSLNAFVNKALEKEVSNHRAGA

Download         Length: 348 bp

>AT70997 NZ_CP018950:2214-2561 [Escherichia coli]
ATGAAACATTTAAAATATAAAGGATATTTAGGCACGGTTGAACCCGATTTTGAAAATAATGTCCTGTATGGAAAACTGGC
GTTTATTCGGGACCTGGTAACTTATGAGGCTTCTACATTAGCTGAACTGGAACAGGAGTTTAAAACATCTGTTGATCTGT
ATTTACAGTCTTGTGTGGAGGATGGAAAGGAACCTGATACCCCGTTTAAAGGCGTGTTTAACGTCAGGCTTGATCCAGAA
CTGCATCGTCGGGTAGCAGAAATGGCGATGGAAGAAGATTTATCGTTGAATGCCTTTGTTAATAAGGCTCTGGAAAAAGA
AGTCAGCAATCATCGCGCAGGGGCTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829CIX3

Antitoxin

Source ID Structure
AlphaFold DB A0A829CLC1

References