70996

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) TsxAB/MqsA(antitoxin)
Location 1..466 Replicon plasmid pEC276_3
Accession NZ_CP018950
Organism Escherichia coli strain Ecol_276

Toxin (Protein)


Gene name TsxA Uniprot ID U9ZUY2
Locus tag BE964_RS00110 Protein ID WP_001230707.1
Coordinates 1..252 (+) Length 84 a.a.

Antitoxin (Protein)


Gene name TsxB Uniprot ID M7CCF8
Locus tag BE964_RS00115 Protein ID WP_000801440.1
Coordinates 239..466 (+) Length 76 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
BE964_RS00110 1..252 + 252 WP_001230707.1 hypothetical protein Toxin
BE964_RS00115 239..466 + 228 WP_000801440.1 hypothetical protein Antitoxin
BE964_RS00125 920..1219 + 300 WP_000650310.1 hypothetical protein -
BE964_RS00130 1314..1664 + 351 WP_000854260.1 hypothetical protein -
BE964_RS00135 1969..2217 + 249 WP_000520549.1 type II toxin-antitoxin system HicA family toxin -
BE964_RS00140 2214..2561 + 348 WP_000681613.1 type II toxin-antitoxin system HicB family antitoxin -
BE964_RS00150 2925..3428 + 504 WP_000781817.1 hypothetical protein -
BE964_RS00155 3432..4661 + 1230 WP_001329504.1 relaxase/mobilization nuclease domain-containing protein -
BE964_RS00160 4738..5004 + 267 WP_000160396.1 hypothetical protein -
BE964_RS00165 5009..5242 + 234 WP_000121161.1 EexN family lipoprotein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - - 1..35225 35225


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(6-85)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(2-72)


Sequences

Toxin        


Download         Length: 84 a.a.        Molecular weight: 9751.13 Da        Isoelectric Point: 8.9187

>T70996 WP_001230707.1 NZ_CP018950:1-252 [Escherichia coli]
MRKNKGDVTYFLEKEGDNYRLTKRIKARTNVKIGNKTTKITLYDAVLNENELQHIDFTCAGLREDDETPVKNLIKEFMLN
ETR

Download         Length: 252 bp

>T70996 NZ_CP018950:1-252 [Escherichia coli]
ATGAGAAAAAATAAAGGTGATGTGACTTATTTTCTTGAAAAAGAAGGTGACAATTACAGGCTTACAAAAAGGATAAAAGC
CAGAACAAATGTTAAAATCGGGAACAAAACTACGAAAATTACATTGTATGATGCCGTACTGAATGAGAATGAATTACAAC
ATATTGATTTTACGTGTGCCGGATTAAGGGAAGACGATGAGACGCCAGTTAAGAATTTAATTAAGGAGTTTATGTTAAAT
GAAACCCGATAA

Antitoxin


Download         Length: 76 a.a.        Molecular weight: 8659.98 Da        Isoelectric Point: 8.5105

>AT70996 WP_000801440.1 NZ_CP018950:239-466 [Escherichia coli]
MKPDNTPENVKKLRLKAGLTQKECSHIYGVGLRTWQKKEEVNTQNSQSLSLVEFEFLLLLAGEHPEYVLCKRESK

Download         Length: 228 bp

>AT70996 NZ_CP018950:239-466 [Escherichia coli]
ATGAAACCCGATAATACACCTGAAAATGTAAAAAAATTACGTTTAAAAGCAGGATTAACACAGAAAGAATGCAGTCATAT
TTATGGTGTTGGGCTAAGAACCTGGCAGAAAAAAGAGGAGGTTAATACTCAAAACAGTCAAAGTCTGTCACTGGTTGAGT
TTGAGTTTCTTTTGTTACTTGCAGGAGAACATCCTGAATACGTGCTCTGCAAAAGGGAGTCAAAATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB U9ZUY2

Antitoxin

Source ID Structure
AlphaFold DB A0A5Y6U8W9

References