Detailed information of TA system TA070994

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 4618724..4619559
• Replicon: chromosome
• Accession: NZ_CP018948
• Organism: Escherichia coli strain Ecol_224

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: Q707F1
• Locus tag: BE957_RS26430
• Protein ID: WP_001094445.1
• Coordinates: 4619182..4619559 (+)
• Length: 126 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: -
• Locus tag: BE957_RS26425
• Protein ID: WP_024174331.1
• Coordinates: 4618724..4619092 (+)
• Length: 123 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BE957_RS26400	4616176..4616956	+	781	Protein_4429	IS3 family transposase	-
BE957_RS26405	4616959..4617291	+	333	Protein_4430	DUF932 domain-containing protein	-
BE957_RS26410	4617383..4617868	+	486	WP_000214415.1	antirestriction protein	-
BE957_RS26415	4617884..4618360	+	477	WP_001186725.1	RadC family protein	-
BE957_RS26420	4618429..4618650	+	222	WP_000692346.1	DUF987 domain-containing protein	-
BE957_RS26425	4618724..4619092	+	369	WP_024174331.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
BE957_RS26430	4619182..4619559	+	378	WP_001094445.1	TA system toxin CbtA family protein	Toxin
BE957_RS26435	4619556..4620044	+	489	WP_000761717.1	hypothetical protein	-
BE957_RS26440	4620056..4620253	+	198	WP_000838536.1	DUF957 domain-containing protein	-
BE957_RS26445	4620338..4621180	+	843	WP_001280499.1	DUF4942 domain-containing protein	-
BE957_RS26455	4621929..4623467	+	1539	WP_001187170.1	lysine decarboxylation/transport transcriptional activator CadC	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	4601728..4631280	29552
	-	flank	IS/Tn	-	-	4616176..4616454	278

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (19-117)

Sequences

Toxin

Protein

• Length: 126 a.a.
• Molecular weight: 14194.10 Da
• Isoelectric Point: 6.8518

>T70994 WP_001094445.1 NZ_CP018948:4619182-4619559 [Escherichia coli]
MNTLPDTHVREASRCPSPVTIWQTLLTRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
SACTRSQLINSIDILRARRATGLMTRDNYRMVNNITQGKHPEAQQ

Nucleotide

• Length: 378 bp

>T70994 NZ_CP018948:4619182-4619559 [Escherichia coli]
ATGAACACATTACCCGACACACACGTACGGGAGGCATCGCGCTGCCCGTCTCCCGTCACCATCTGGCAGACACTGCTCAC
CCGACTGCTGGACCAGCACTACGGCCTCACGCTGAATGACACACCGTTCGCCGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGCATTTCACTGTGTGATGCGGTGAACTTTCTCGTGGAAAAATACGCGCTGGTACGAACTGACCAGCCAGGATTC
AGCGCCTGTACCCGCTCTCAGTTAATAAACAGCATCGATATCCTCCGGGCTCGCCGGGCAACAGGCCTGATGACCCGCGA
CAATTACAGAATGGTAAATAACATTACCCAGGGTAAGCATCCGGAGGCGCAACAATGA

Antitoxin

Protein

• Length: 123 a.a.
• Molecular weight: 13603.42 Da
• Isoelectric Point: 6.7386

>AT70994 WP_024174331.1 NZ_CP018948:4618724-4619092 [Escherichia coli]
VSDTLPGTTHPDDNHDRLWWGLPCTVTPCFGARLVQKGNRLHYLADRAGIRGRFSDADAYHLDQAFPLLMKQLELMLTSG
ELNPRHQHTVTLYAKGLTCEADTLGSCGYVYLAVYPTPETKT

Nucleotide

• Length: 369 bp

>AT70994 NZ_CP018948:4618724-4619092 [Escherichia coli]
GTGTCAGACACACTGCCCGGGACAACGCATCCCGACGACAATCACGACCGCCTCTGGTGGGGACTGCCCTGCACCGTGAC
GCCCTGTTTCGGGGCACGTCTGGTGCAGAAGGGTAACCGGTTGCATTACCTTGCCGACCGCGCCGGTATCAGAGGCCGGT
TCAGCGATGCAGATGCATACCACCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAACTCATGCTCACCAGCGGT
GAACTGAATCCCCGCCATCAGCATACCGTGACACTGTATGCGAAGGGGCTGACCTGTGAAGCCGACACCCTCGGCTCCTG
TGGTTACGTTTATCTGGCTGTTTATCCGACGCCCGAAACGAAAACGTAA

Structures

Toxin

• AlphaFold DB: A0A454A7U6