Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/UPF0150(antitoxin)
Location 2694548..2695186 Replicon chromosome
Accession NZ_CP018948
Organism Escherichia coli strain Ecol_224

Toxin (Protein)


Gene name hicA Uniprot ID S1F9G9
Locus tag BE957_RS16260 Protein ID WP_000813794.1
Coordinates 2695010..2695186 (-) Length 59 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID -
Locus tag BE957_RS16255 Protein ID WP_001270286.1
Coordinates 2694548..2694964 (-) Length 139 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
BE957_RS16235 2689700..2690641 - 942 WP_001251315.1 ABC transporter permease -
BE957_RS16240 2690642..2691655 - 1014 WP_000220396.1 ABC transporter ATP-binding protein -
BE957_RS16245 2691673..2692818 - 1146 WP_000047456.1 ABC transporter substrate-binding protein -
BE957_RS16250 2693063..2694469 - 1407 WP_000760592.1 PLP-dependent aminotransferase family protein -
BE957_RS16255 2694548..2694964 - 417 WP_001270286.1 type II toxin-antitoxin system antitoxin HicB Antitoxin
BE957_RS16260 2695010..2695186 - 177 WP_000813794.1 type II toxin-antitoxin system mRNA interferase toxin HicA Toxin
BE957_RS16265 2695408..2695638 + 231 WP_000494244.1 YncJ family protein -
BE957_RS16270 2695730..2697691 - 1962 WP_001593323.1 23S rRNA 5-hydroxycytidine C2501 synthase -
BE957_RS16275 2697764..2698300 - 537 WP_000429155.1 DNA-binding transcriptional regulator SutR -
BE957_RS16280 2698353..2699567 + 1215 WP_001348055.1 BenE family transporter YdcO -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- flank IS/Tn - - 2699607..2700755 1148


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(5-57)

Antitoxin

Cro/C1-type helix-turn-helix domain

(91-134)


Sequences

Toxin        


Download         Length: 59 a.a.        Molecular weight: 6749.83 Da        Isoelectric Point: 11.2298

>T70989 WP_000813794.1 NZ_CP018948:c2695186-2695010 [Escherichia coli]
VKQSEFRRWLESQGVDVANGSNHLKLRFHGRRSVMPRHPCDEIKEPLRKAILKQLGLS

Download         Length: 177 bp

>T70989 NZ_CP018948:c2695186-2695010 [Escherichia coli]
GTGAAACAAAGCGAGTTCAGACGTTGGCTCGAATCTCAGGGCGTCGATGTAGCGAATGGCAGCAACCATTTGAAACTCAG
GTTTCATGGGAGGCGCAGTGTCATGCCGCGTCACCCCTGCGATGAGATTAAAGAACCATTGCGTAAAGCAATCCTGAAAC
AACTCGGTTTGAGTTAA

Antitoxin


Download         Length: 139 a.a.        Molecular weight: 15246.63 Da        Isoelectric Point: 4.7386

>AT70989 WP_001270286.1 NZ_CP018948:c2694964-2694548 [Escherichia coli]
MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALLTAFDFYFEDNELIPLPSPLNSHDHFIEVPLSVASKV
LLLNAFLQSEITQQELARRIGKPKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV

Download         Length: 417 bp

>AT70989 NZ_CP018948:c2694964-2694548 [Escherichia coli]
ATGCGTTATCCCGTCACTCTTACACCCGCGCCGGAAGGCGGTTATATGGTTTCTTTTGTGGATATCCCTGAAGCGTTGAC
GCAGGGCGAAACTGTCGCTGAAGCGATGGAAGCGGCAAAAGATGCTTTACTGACCGCATTTGATTTTTATTTTGAAGATA
ACGAGCTTATCCCTTTACCTTCGCCATTAAATAGTCATGATCACTTTATTGAAGTACCTTTGAGCGTCGCCTCTAAGGTA
TTGCTGTTAAATGCTTTTTTACAGTCAGAAATCACTCAGCAAGAGTTAGCCAGGCGAATTGGCAAACCTAAACAGGAGAT
TACTCGCCTATTTAACTTGCATCATGCGACAAAAATCGACGCCGTCCAGCTCGCGGCAAAGGCGCTTGGCAAAGAGTTAT
CGCTGGTGATGGTTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6HPB

Antitoxin

Source ID Structure

References