Detailed information of TA system TA070979

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 2082879..2083714
• Replicon: chromosome
• Accession: NZ_CP018948
• Organism: Escherichia coli strain Ecol_224

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: A0A1U9SHU4
• Locus tag: BE957_RS12940
• Protein ID: WP_000854752.1
• Coordinates: 2082879..2083256 (-)
• Length: 126 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: E3PJ72
• Locus tag: BE957_RS12945
• Protein ID: WP_001278232.1
• Coordinates: 2083346..2083714 (-)
• Length: 123 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BE957_RS12910	2078399..2078872	+	474	WP_001105384.1	DNA gyrase inhibitor SbmC	-
BE957_RS12915	2079070..2080128	+	1059	WP_001200892.1	FUSC family protein	-
BE957_RS12920	2080300..2080629	+	330	WP_000450409.1	DUF496 family protein	-
BE957_RS12925	2080730..2080912	-	183	WP_024167529.1	ethanolamine utilization protein	-
BE957_RS28525	2081366..2081737	-	372	WP_001295631.1	IS110 family transposase	-
BE957_RS27975	2082553..2082666	-	114	WP_001161660.1	DUF957 domain-containing protein	-
BE957_RS12935	2082679..2082882	-	204	WP_000761705.1	hypothetical protein	-
BE957_RS12940	2082879..2083256	-	378	WP_000854752.1	TA system toxin CbtA family protein	Toxin
BE957_RS12945	2083346..2083714	-	369	WP_001278232.1	type IV toxin-antitoxin system toxin CbtA	Antitoxin
BE957_RS12955	2083877..2084098	-	222	WP_000692329.1	DUF987 domain-containing protein	-
BE957_RS12960	2084161..2084637	-	477	WP_001347688.1	RadC family protein	-
BE957_RS12965	2084653..2085138	-	486	WP_000849582.1	antirestriction protein	-
BE957_RS12970	2085193..2086011	-	819	WP_001234620.1	DUF945 domain-containing protein	-
BE957_RS12980	2086111..2086344	-	234	WP_001119729.1	DUF905 family protein	-
BE957_RS12985	2086423..2086878	-	456	WP_000581504.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (18-117)

Sequences

Toxin

Protein

• Length: 126 a.a.
• Molecular weight: 14076.08 Da
• Isoelectric Point: 8.2905

>T70979 WP_000854752.1 NZ_CP018948:c2083256-2082879 [Escherichia coli]
MKTLPDTHVREASSCPSPVTIWQTLLSRLLGQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
STCPRSQLINSIDILRARRATGLMTRDNYRTVNNITLGKHPEAKR

Nucleotide

• Length: 378 bp

>T70979 NZ_CP018948:c2083256-2082879 [Escherichia coli]
ATGAAAACATTACCCGATACACACGTACGGGAGGCATCGAGCTGCCCGTCCCCCGTCACCATCTGGCAGACTCTGCTCTC
CCGACTACTGGGCCAGCATTACGGCCTTACACTGAATGACACACCGTTCGCCGATGAACGTGTGATTGAGCAGCATATTG
AAGCTGGCATTTCACTGTGTGATGCGGTGAATTTTCTCGTGGAAAAATACGCGCTGGTGCGTACCGACCAGCCGGGATTC
AGCACTTGTCCCCGCTCTCAGTTAATAAACAGTATTGATATCCTCCGGGCTCGCCGGGCAACAGGCCTGATGACCCGCGA
CAATTACAGAACGGTAAATAACATTACCCTGGGGAAGCATCCGGAGGCGAAACGATGA

Antitoxin

Protein

• Length: 123 a.a.
• Molecular weight: 13491.37 Da
• Isoelectric Point: 6.6290

>AT70979 WP_001278232.1 NZ_CP018948:c2083714-2083346 [Escherichia coli]
VSDALSGTMLPDDNHDRPWWGLPCTVTPCFGARLVQEGNRLHYLADRAGIRGLFSDADAYHLDQAFPLLMKQLELMLTSG
ELNPRHQHTVTLYAKGLTCKADTLGSGGYVYLAVYPTPETKK

Nucleotide

• Length: 369 bp

>AT70979 NZ_CP018948:c2083714-2083346 [Escherichia coli]
GTGTCAGACGCACTCTCCGGGACAATGCTTCCCGACGACAATCACGACCGCCCCTGGTGGGGGCTGCCCTGCACCGTGAC
GCCCTGTTTCGGGGCACGTCTGGTGCAGGAGGGTAACCGCCTGCATTACCTTGCCGACCGCGCCGGTATCAGAGGCCTGT
TCAGCGATGCAGATGCGTACCACCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAACTCATGCTCACCAGCGGT
GAACTGAATCCCCGCCATCAGCATACCGTCACGCTGTATGCAAAAGGTCTGACCTGCAAAGCTGACACTCTCGGTAGCGG
TGGTTACGTTTATCTGGCTGTTTATCCGACGCCCGAAACGAAAAAGTAA

Structures

Toxin

• AlphaFold DB: A0A1U9SHU4

Antitoxin

• AlphaFold DB: E3PJ72