Detailed information of TA system TA070977

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VagC
• Location: 1534608..1535233
• Replicon: chromosome
• Accession: NZ_CP018948
• Organism: Escherichia coli strain Ecol_224

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: BE957_RS10170
• Protein ID: WP_000911330.1
• Coordinates: 1534835..1535233 (+)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: U9XQV3
• Locus tag: BE957_RS10165
• Protein ID: WP_000450524.1
• Coordinates: 1534608..1534835 (+)
• Length: 76 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BE957_RS10140	1530411..1530881	-	471	WP_001068682.1	thioredoxin-dependent thiol peroxidase	-
BE957_RS10145	1530881..1531453	-	573	WP_000176187.1	glycine cleavage system transcriptional repressor	-
BE957_RS10150	1531599..1532477	+	879	WP_001295469.1	4-hydroxy-tetrahydrodipicolinate synthase	-
BE957_RS10155	1532494..1533528	+	1035	WP_001330579.1	outer membrane protein assembly factor BamC	-
BE957_RS10160	1533741..1534454	+	714	WP_001347624.1	phosphoribosylaminoimidazolesuccinocarboxamide synthase	-
BE957_RS10165	1534608..1534835	+	228	WP_000450524.1	toxin-antitoxin system antitoxin VapB	Antitoxin
BE957_RS10170	1534835..1535233	+	399	WP_000911330.1	tRNA(fMet)-specific endonuclease VapC	Toxin
BE957_RS10175	1535380..1536243	+	864	WP_001267498.1	neutral zinc metallopeptidase	-
BE957_RS10180	1536258..1538273	+	2016	WP_000829292.1	tRNA cytosine(34) acetyltransferase TmcA	-
BE957_RS10185	1538347..1539045	+	699	WP_000679812.1	esterase	-
BE957_RS10190	1539126..1539326	-	201	WP_000383836.1	YpfN family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-125)

Antitoxin

• SpoVT-AbrB domain (7-52)

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 14894.25 Da
• Isoelectric Point: 9.2216

>T70977 WP_000911330.1 NZ_CP018948:1534835-1535233 [Escherichia coli]
MLKFMLDTNICIFTIKNKPVHVRERFNLNSGRMCISSVTLMELIYGAEKSQMPERNLAVIEGFVSRLVVLDYDSAAATHT
GQIRAELARQGRPVGPFDQMIAGHARSRGLIVVTNNTREFERVAGIRLEDWS

Nucleotide

• Length: 399 bp

>T70977 NZ_CP018948:1534835-1535233 [Escherichia coli]
ATGCTGAAATTCATGCTGGATACCAATATCTGTATTTTCACTATTAAGAATAAACCAGTACATGTCAGAGAGCGGTTTAA
CCTGAATTCTGGCCGGATGTGTATAAGTTCTGTAACATTGATGGAGCTTATCTATGGGGCAGAAAAAAGTCAGATGCCAG
AACGCAATTTGGCGGTTATTGAAGGTTTCGTATCTCGTTTGGTAGTGCTTGATTATGATAGTGCTGCTGCGACGCATACC
GGGCAAATCAGGGCCGAGCTTGCCCGGCAGGGAAGGCCTGTTGGACCTTTTGACCAGATGATAGCGGGACATGCTCGCAG
TCGCGGTTTGATTGTGGTTACCAACAATACCCGAGAGTTTGAGCGAGTGGCGGGAATCAGACTAGAAGACTGGAGCTAA

Antitoxin

Protein

• Length: 76 a.a.
• Molecular weight: 8498.61 Da
• Isoelectric Point: 4.3867

>AT70977 WP_000450524.1 NZ_CP018948:1534608-1534835 [Escherichia coli]
METTVFLSNRSQAVRLPKAVALPEDVKKVEIIAIGRTRIITPAGESWDSWFDGENVSADFMDIRDQPAMQERESF

Nucleotide

• Length: 228 bp

>AT70977 NZ_CP018948:1534608-1534835 [Escherichia coli]
ATGGAAACTACTGTATTTTTAAGTAATCGCAGTCAGGCCGTCAGATTACCGAAAGCGGTCGCTCTGCCAGAAGACGTTAA
GAAAGTAGAAATTATTGCGATTGGTCGTACAAGAATCATCACTCCCGCCGGTGAGTCCTGGGACAGTTGGTTTGATGGCG
AAAATGTGAGTGCGGATTTTATGGATATCCGTGATCAACCCGCAATGCAGGAAAGGGAATCATTTTGA

Structures

Antitoxin

• AlphaFold DB: A0A829CM33