Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) phd-doc/Doc-Phd
Location 124774..125375 Replicon plasmid pEC224_1
Accession NZ_CP018947
Organism Escherichia coli strain Ecol_224

Toxin (Protein)


Gene name doc Uniprot ID V0AJ64
Locus tag BE957_RS02130 Protein ID WP_001216034.1
Coordinates 124774..125154 (-) Length 127 a.a.

Antitoxin (Protein)


Gene name phd Uniprot ID U9YQH9
Locus tag BE957_RS02135 Protein ID WP_001190712.1
Coordinates 125154..125375 (-) Length 74 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
BE957_RS02095 119825..120757 - 933 WP_000081352.1 homocysteine S-methyltransferase -
BE957_RS02100 120744..122147 - 1404 WP_001373486.1 S-methylmethionine permease -
BE957_RS02105 122391..123371 + 981 WP_000019407.1 IS5-like element IS5 family transposase -
BE957_RS02110 123599..123916 + 318 WP_001513659.1 hypothetical protein -
BE957_RS02115 123866..124279 - 414 Protein_154 DDE-type integrase/transposase/recombinase -
BE957_RS02120 124203..124562 - 360 WP_001513660.1 hypothetical protein -
BE957_RS02125 124590..124769 - 180 WP_001513661.1 hypothetical protein -
BE957_RS02130 124774..125154 - 381 WP_001216034.1 type II toxin-antitoxin system death-on-curing family toxin Toxin
BE957_RS02135 125154..125375 - 222 WP_001190712.1 type II toxin-antitoxin system Phd/YefM family antitoxin Antitoxin
BE957_RS02140 125558..127114 + 1557 WP_001553856.1 type I restriction-modification system subunit M -
BE957_RS02145 127111..128394 + 1284 WP_001553855.1 restriction endonuclease subunit S -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid catA1 / tet(B) - 1..143972 143972


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Death on curing protein

(2-123)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-51)


Sequences

Toxin        


Download         Length: 127 a.a.        Molecular weight: 13615.32 Da        Isoelectric Point: 5.1514

>T70970 WP_001216034.1 NZ_CP018947:c125154-124774 [Escherichia coli]
MRHISPEELIALHDANINRYGGLPGMSDPGRAEAIIGRVQARVAYEEITDLFEVSATYLVATARGHIFNDANKRTALNSA
LLFLRRNGVQVFDSPELADLTVGAATGEISVSSVADTLRRLYGSAE

Download         Length: 381 bp

>T70970 NZ_CP018947:c125154-124774 [Escherichia coli]
ATGAGGCATATATCACCGGAAGAACTTATTGCGCTTCATGATGCGAATATAAACCGCTACGGCGGCCTGCCGGGAATGTC
TGATCCGGGCAGGGCAGAGGCCATTATCGGGAGAGTTCAGGCCAGAGTTGCCTACGAAGAGATCACCGACCTTTTCGAAG
TCTCCGCCACCTACCTAGTGGCTACAGCGAGAGGGCATATATTCAATGATGCCAATAAGCGTACCGCGCTAAACAGTGCG
CTGTTATTTCTACGCCGTAACGGGGTGCAGGTATTTGATTCACCTGAACTGGCAGACCTTACCGTAGGGGCTGCGACCGG
AGAGATATCTGTATCTTCTGTCGCCGACACGTTACGTAGATTGTATGGTTCTGCGGAGTAG

Antitoxin


Download         Length: 74 a.a.        Molecular weight: 8133.10 Da        Isoelectric Point: 4.7708

>AT70970 WP_001190712.1 NZ_CP018947:c125375-125154 [Escherichia coli]
MQSINFRTARGNLSEVLNNVEAGEEVEITRRGREPAVIVSKATFEAYKKAALDAEFASLFDTLDSTNKELVNR

Download         Length: 222 bp

>AT70970 NZ_CP018947:c125375-125154 [Escherichia coli]
ATGCAATCCATTAACTTCCGTACCGCGCGCGGCAACCTTTCTGAAGTGCTCAACAATGTTGAGGCCGGGGAAGAGGTTGA
AATCACCCGCAGAGGCCGTGAGCCAGCAGTAATTGTCAGCAAGGCTACTTTCGAAGCCTACAAAAAAGCGGCGCTGGATG
CTGAATTTGCATCCCTGTTTGACACCCTGGACTCCACCAACAAGGAACTGGTTAACCGATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB V0AJ64

Antitoxin

Source ID Structure
AlphaFold DB A0A829CJB6

References