Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) relBE/RHH(antitoxin)
Location 110900..111459 Replicon plasmid pEC224_1
Accession NZ_CP018947
Organism Escherichia coli strain Ecol_224

Toxin (Protein)


Gene name relE Uniprot ID V0SVC8
Locus tag BE957_RS02025 Protein ID WP_000421272.1
Coordinates 111184..111459 (+) Length 92 a.a.

Antitoxin (Protein)


Gene name relB Uniprot ID V0SRR3
Locus tag BE957_RS02020 Protein ID WP_001387467.1
Coordinates 110900..111184 (+) Length 95 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
BE957_RS01990 106363..106803 - 441 Protein_133 zinc-binding dehydrogenase -
BE957_RS02000 107204..108094 + 891 WP_001077068.1 dihydrodipicolinate synthase family protein -
BE957_RS02005 108119..108499 + 381 WP_000710783.1 Rid family detoxifying hydrolase -
BE957_RS02010 108532..109497 + 966 WP_001022265.1 2-keto-3-deoxygluconate transporter -
BE957_RS02015 109543..110295 - 753 WP_000562172.1 IclR family transcriptional regulator -
BE957_RS02020 110900..111184 + 285 WP_001387467.1 ribbon-helix-helix domain-containing protein Antitoxin
BE957_RS02025 111184..111459 + 276 WP_000421272.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
BE957_RS02030 111565..111858 + 294 WP_001105060.1 hypothetical protein -
BE957_RS27825 111893..112021 - 129 Protein_141 IS481 family transposase -
BE957_RS02035 112054..113223 - 1170 WP_001072355.1 hypothetical protein -
BE957_RS02045 113716..114023 + 308 Protein_143 type II toxin-antitoxin system ParD family antitoxin -
BE957_RS27830 114366..114548 + 183 WP_042065278.1 hypothetical protein -
BE957_RS02060 114669..115409 + 741 WP_001066942.1 tyrosine-type recombinase/integrase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid catA1 / tet(B) - 1..143972 143972


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(3-90)

Antitoxin

Ribbon-helix-helix protein, CopG

(14-51)


Sequences

Toxin        


Download         Length: 92 a.a.        Molecular weight: 10749.45 Da        Isoelectric Point: 10.0871

>T70969 WP_000421272.1 NZ_CP018947:111184-111459 [Escherichia coli]
MELKWTSKALSDLSRLFDFLVLASKPAAARTVQSLTQAPVILLTHPRMGEQLFQFEPREVRRIFAGEYEIRYEINGQTIY
VLRLWHTRENR

Download         Length: 276 bp

>T70969 NZ_CP018947:111184-111459 [Escherichia coli]
ATGGAACTGAAGTGGACCAGTAAGGCGCTTTCTGATTTGTCGCGGTTATTTGATTTTCTGGTGCTGGCCAGTAAACCTGC
TGCCGCCAGAACGGTACAGTCCCTGACACAGGCACCGGTCATTCTGTTAACTCATCCACGTATGGGAGAACAGTTGTTTC
AGTTTGAACCCAGGGAGGTCAGACGGATTTTTGCTGGCGAGTACGAAATCCGTTACGAAATTAATGGCCAGACTATTTAT
GTATTGCGTCTGTGGCACACACGAGAAAACAGGTAG

Antitoxin


Download         Length: 95 a.a.        Molecular weight: 10460.87 Da        Isoelectric Point: 5.6558

>AT70969 WP_001387467.1 NZ_CP018947:110900-111184 [Escherichia coli]
MQMKNNAAQATKVITAHVPLPMADKVDQMAARLERSRGWVIKQALSAWLAQEEERNRLTLEALDDVTSGQVIDHQAVQAW
SDSLSTDNPLPVPR

Download         Length: 285 bp

>AT70969 NZ_CP018947:110900-111184 [Escherichia coli]
ATGCAAATGAAAAACAATGCCGCACAAGCAACAAAAGTAATTACCGCGCATGTGCCATTACCTATGGCTGATAAAGTCGA
CCAGATGGCCGCCAGACTGGAACGTTCCCGGGGCTGGGTTATCAAACAGGCGCTTTCTGCATGGCTTGCCCAGGAGGAGG
AGCGTAATCGCCTGACGCTGGAAGCCCTGGACGATGTGACATCCGGACAGGTTATCGACCATCAGGCTGTACAGGCCTGG
TCGGACAGCCTCAGTACTGACAATCCGTTACCGGTGCCACGCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB V0SVC8

Antitoxin

Source ID Structure
AlphaFold DB A0A0E0Y880

References