Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) parDE/RelE-RHH
Location 39160..39707 Replicon plasmid pEC224_2
Accession NZ_CP018946
Organism Escherichia coli strain Ecol_224

Toxin (Protein)


Gene name parE Uniprot ID E9LLH3
Locus tag BE957_RS00915 Protein ID WP_000969996.1
Coordinates 39160..39441 (-) Length 94 a.a.

Antitoxin (Protein)


Gene name parD Uniprot ID A0A0S0ZPX5
Locus tag BE957_RS00920 Protein ID WP_032291093.1
Coordinates 39438..39707 (-) Length 90 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
BE957_RS28225 34237..34539 + 303 WP_001245884.1 hypothetical protein -
BE957_RS00890 35090..36436 - 1347 WP_001132021.1 hypothetical protein -
BE957_RS00900 36789..37391 + 603 WP_078207111.1 proQ/FINO family protein -
BE957_RS00905 37447..37956 + 510 WP_032291091.1 hypothetical protein -
BE957_RS00910 38272..39120 - 849 WP_032291092.1 3'-5' exonuclease -
BE957_RS00915 39160..39441 - 282 WP_000969996.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
BE957_RS00920 39438..39707 - 270 WP_032291093.1 hypothetical protein Antitoxin
BE957_RS28230 39762..39920 - 159 WP_038349023.1 hypothetical protein -
BE957_RS00940 40618..41649 - 1032 WP_052991836.1 replication initiation protein -
BE957_RS00955 42622..42885 + 264 WP_001387472.1 ash family protein -
BE957_RS00960 42803..43090 + 288 WP_000074855.1 conjugal transfer protein TraA -
BE957_RS00970 43532..44065 + 534 WP_001372168.1 transcription termination factor NusG -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid - - 1..87114 87114


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(4-92)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 94 a.a.        Molecular weight: 10893.57 Da        Isoelectric Point: 8.4615

>T70963 WP_000969996.1 NZ_CP018946:c39441-39160 [Escherichia coli]
MMEIFWTMLASQDRKRIREYIAEQNLMAAIELDERIGYSASSLAGQPYKGRNGRVEGTRELVIHPHFVLVYEVDSQWGKV
YILRVLHTAQKWP

Download         Length: 282 bp

>T70963 NZ_CP018946:c39441-39160 [Escherichia coli]
ATGATGGAGATATTCTGGACCATGCTGGCCAGCCAGGACAGGAAGCGCATTCGTGAGTACATTGCGGAGCAGAACCTGAT
GGCCGCCATTGAACTGGACGAACGGATTGGTTATTCAGCCAGCAGCCTGGCCGGGCAGCCGTATAAAGGCCGCAACGGTC
GTGTGGAAGGTACCCGGGAACTGGTTATTCACCCGCATTTTGTTCTGGTTTATGAGGTCGACAGCCAGTGGGGAAAAGTG
TATATCCTGCGCGTGTTGCATACTGCACAGAAGTGGCCATAG

Antitoxin


Download         Length: 90 a.a.        Molecular weight: 10069.43 Da        Isoelectric Point: 8.0565

>AT70963 WP_032291093.1 NZ_CP018946:c39707-39438 [Escherichia coli]
MAQVNMSLRIDAELKDAFMAAAKSMDRNGSQLIRDFMRQTVERQHNTWFRDQVAAGRQQLGCGDVLPHDMVESSAAAWRD
EMSRKVAGK

Download         Length: 270 bp

>AT70963 NZ_CP018946:c39707-39438 [Escherichia coli]
ATGGCACAGGTTAATATGAGTTTAAGAATCGACGCTGAACTGAAGGATGCTTTTATGGCCGCTGCAAAAAGCATGGACCG
TAATGGCTCTCAGTTAATCCGGGATTTTATGCGCCAGACCGTTGAACGGCAGCATAATACCTGGTTCCGTGACCAGGTTG
CGGCAGGACGTCAGCAACTCGGGTGCGGCGATGTGCTTCCCCATGACATGGTCGAATCTTCCGCTGCTGCATGGCGGGAT
GAAATGAGCAGGAAGGTTGCCGGTAAATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB E9LLH3

Antitoxin

Source ID Structure
AlphaFold DB A0A0S0ZPX5

References