70957

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) pemIK/MazF(toxin)
Location 1470112..1470754 Replicon chromosome
Accession NZ_CP018935
Organism Bacillus cereus strain JEM-2

Toxin (Protein)


Gene name pemK Uniprot ID J8CWW5
Locus tag BEI53_RS07875 Protein ID WP_000635963.1
Coordinates 1470112..1470462 (-) Length 117 a.a.

Antitoxin (Protein)


Gene name pemI Uniprot ID R8I8H2
Locus tag BEI53_RS07880 Protein ID WP_000004570.1
Coordinates 1470467..1470754 (-) Length 96 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
BEI53_RS07860 1467048..1467506 - 459 WP_000344248.1 SprT family protein -
BEI53_RS07865 1467702..1467818 + 117 WP_001143642.1 cortex morphogenetic protein CmpA -
BEI53_RS07870 1467876..1470044 - 2169 WP_000426225.1 RNA-binding transcriptional accessory protein -
BEI53_RS07875 1470112..1470462 - 351 WP_000635963.1 type II toxin-antitoxin system endoribonuclease NdoA Toxin
BEI53_RS07880 1470467..1470754 - 288 WP_000004570.1 antitoxin EndoAI Antitoxin
BEI53_RS07885 1471064..1472233 - 1170 WP_075396319.1 alanine racemase -
BEI53_RS07890 1472352..1473302 - 951 WP_025388382.1 outer membrane lipoprotein carrier protein LolA -
BEI53_RS07895 1473459..1473818 - 360 WP_000635040.1 holo-ACP synthase -
BEI53_RS07900 1473911..1474483 + 573 WP_000906926.1 rhomboid family intramembrane serine protease -
BEI53_RS07905 1474476..1475438 - 963 WP_000961155.1 UV DNA damage repair endonuclease UvsE -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(1-113)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 12974.12 Da        Isoelectric Point: 5.7168

>T70957 WP_000635963.1 NZ_CP018935:c1470462-1470112 [Bacillus cereus]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTVIVAAITAQIQKAKLPTHVEIDAKKYGFERDSVILLEQI
RTIDKQRLTDKITHLDEVMMIRVDEALQISLGLIDF

Download         Length: 351 bp

>T70957 NZ_CP018935:c1470462-1470112 [Bacillus cereus]
TTGATTGTAAAACGCGGCGACGTGTATTTTGCAGACCTTTCCCCAGTTGTTGGTTCTGAGCAAGGAGGCGTTCGTCCGGT
TCTTGTCATTCAAAATGACATCGGAAATCGTTTTAGTCCAACGGTGATTGTAGCGGCTATTACTGCACAGATTCAAAAAG
CGAAATTACCCACTCATGTGGAAATTGATGCGAAAAAGTACGGTTTTGAGAGAGATTCTGTTATTTTACTTGAGCAGATT
CGAACAATCGATAAGCAGCGCTTAACGGACAAAATCACTCACTTAGATGAAGTGATGATGATTCGTGTAGATGAAGCGCT
ACAAATTAGTTTAGGACTAATAGATTTTTAA

Antitoxin


Download         Length: 96 a.a.        Molecular weight: 10780.36 Da        Isoelectric Point: 8.9605

>AT70957 WP_000004570.1 NZ_CP018935:c1470754-1470467 [Bacillus cereus]
VSESSVTTEIVVRLPKQMVTELDGIGKQENKNRHELICQATQLLLRQHKTKKRYQHESMRRGYIEMGKINLGIASEAFLA
EYEAAHTVERLVSGG

Download         Length: 288 bp

>AT70957 NZ_CP018935:c1470754-1470467 [Bacillus cereus]
GTGTCCGAATCAAGTGTAACTACTGAAATCGTGGTTCGGTTGCCAAAGCAAATGGTAACGGAATTGGACGGAATTGGAAA
ACAAGAGAATAAGAATCGCCATGAACTAATTTGCCAGGCAACACAACTGTTATTGCGTCAACATAAGACGAAGAAACGCT
ACCAACATGAATCAATGCGACGTGGGTACATTGAAATGGGAAAAATTAATCTTGGTATTGCATCTGAAGCTTTCTTAGCA
GAGTATGAAGCAGCTCATACAGTAGAACGCTTAGTTAGCGGGGGGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4HKE
PDB 7BXY

Antitoxin

Source ID Structure
AlphaFold DB A0A366FY90

References