Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) /SpoIISA(toxin)
Location 2281314..2282289 Replicon chromosome
Accession NZ_CP018933
Organism Bacillus cereus strain ISSFR-9F

Toxin (Protein)


Gene name spoIISA Uniprot ID C3GJ28
Locus tag BEI49_RS12385 Protein ID WP_003297560.1
Coordinates 2281314..2282051 (+) Length 246 a.a.

Antitoxin (Protein)


Gene name - Uniprot ID R8HWP4
Locus tag BEI49_RS12390 Protein ID WP_000588712.1
Coordinates 2282164..2282289 (+) Length 42 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
BEI49_RS12355 2276439..2277099 + 661 Protein_2254 class I SAM-dependent methyltransferase -
BEI49_RS12360 2277219..2277626 + 408 WP_000072480.1 VOC family protein -
BEI49_RS29650 2277732..2277824 + 93 Protein_2256 methyltransferase -
BEI49_RS12365 2277946..2279631 - 1686 WP_003304987.1 alpha-keto acid decarboxylase family protein -
BEI49_RS12370 2279738..2280220 + 483 WP_000191912.1 MarR family winged helix-turn-helix transcriptional regulator -
BEI49_RS12375 2280388..2281125 + 738 WP_000594156.1 2,3-diphosphoglycerate-dependent phosphoglycerate mutase -
BEI49_RS12385 2281314..2282051 + 738 WP_003297560.1 type II toxin-antitoxin system SpoIISA family toxin Toxin
BEI49_RS12390 2282164..2282289 + 126 WP_000588712.1 hypothetical protein Antitoxin
BEI49_RS12400 2282577..2282858 - 282 WP_001041554.1 hypothetical protein -
BEI49_RS12405 2283135..2283371 - 237 WP_001021688.1 hypothetical protein -
BEI49_RS29655 2283710..2283886 + 177 WP_000808046.1 hypothetical protein -
BEI49_RS12420 2284030..2285499 + 1470 WP_000287522.1 beta-Ala-His dipeptidase -
BEI49_RS12430 2286026..2286496 - 471 WP_029438359.1 DUF5065 family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin SpoIISA, type II toxin-antitoxin system

(9-243)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 246 a.a.        Molecular weight: 28373.07 Da        Isoelectric Point: 8.2672

>T70956 WP_003297560.1 NZ_CP018933:2281314-2282051 [Bacillus cereus]
VISNIRIGLFILAIVFLILVFFYWKNEELYEEKKQRIRKTWYGLFIISVTVYFMIKGIDLTLWKNLLMFTAMVIFVDIAF
ILTPNISEIWGAKFSDIGKTVQSIKRSLIASKARGEIYTTIIQNVNPAVFGTMEWHTEEEYTKSLNAFLDSYGEKIGAKI
VVFEAAKELNTNFRGIRSQFSTIIPLEYIEQLNEQRAVQVENVGIIPAKIVSDVFIVIDGKKNNLQDRDFENVYNLTIHH
SYFSK

Download         Length: 738 bp

>T70956 NZ_CP018933:2281314-2282051 [Bacillus cereus]
GTGATCTCTAACATTCGAATCGGCTTATTTATTTTAGCAATCGTTTTTCTAATACTTGTTTTCTTTTATTGGAAAAACGA
AGAGTTGTACGAGGAGAAGAAGCAACGTATTAGAAAGACTTGGTATGGGTTGTTTATAATATCAGTCACCGTGTATTTCA
TGATAAAAGGAATTGATTTAACCCTCTGGAAAAATCTTTTAATGTTCACTGCAATGGTTATTTTCGTTGATATTGCATTT
ATTTTAACACCTAATATTTCAGAAATATGGGGAGCGAAATTTAGCGATATCGGCAAGACGGTTCAATCGATAAAACGGTC
ATTAATTGCTTCTAAAGCAAGAGGAGAAATATACACAACAATCATTCAAAATGTCAATCCAGCAGTGTTCGGTACGATGG
AATGGCATACGGAGGAAGAATATACAAAAAGCTTAAACGCATTTTTAGATTCGTATGGGGAAAAGATTGGAGCAAAAATT
GTTGTATTTGAAGCGGCAAAGGAATTAAATACAAACTTTCGTGGTATCCGTTCTCAATTTAGTACGATTATTCCGTTAGA
GTACATTGAGCAATTGAATGAGCAGAGAGCAGTACAAGTAGAAAATGTCGGAATTATACCAGCAAAAATAGTGAGTGATG
TTTTCATTGTTATTGATGGGAAGAAAAATAACCTGCAAGATCGAGATTTTGAAAATGTATATAATTTAACAATACATCAT
AGTTATTTTAGTAAATAA

Antitoxin


Download         Length: 42 a.a.        Molecular weight: 4938.34 Da        Isoelectric Point: 3.9150

>AT70956 WP_000588712.1 NZ_CP018933:2282164-2282289 [Bacillus cereus]
VIKDYDNDFFKEDNEDTTDFSLIKGDTLQQVEELEEELKRK

Download         Length: 126 bp

>AT70956 NZ_CP018933:2282164-2282289 [Bacillus cereus]
GTGATAAAAGATTATGACAATGATTTTTTTAAAGAAGATAATGAAGATACAACAGATTTCTCGTTAATAAAAGGAGATAC
ATTACAGCAAGTAGAAGAATTAGAAGAAGAATTAAAAAGAAAATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A3R9EK80

Antitoxin

Source ID Structure
AlphaFold DB A0A366GQT1

References