Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) pemIK/MazF(toxin)
Location 186435..187077 Replicon chromosome
Accession NZ_CP018933
Organism Bacillus cereus strain ISSFR-9F

Toxin (Protein)


Gene name pemK Uniprot ID J8CWW5
Locus tag BEI49_RS01110 Protein ID WP_000635963.1
Coordinates 186727..187077 (+) Length 117 a.a.

Antitoxin (Protein)


Gene name pemI Uniprot ID R8I8H2
Locus tag BEI49_RS01105 Protein ID WP_000004570.1
Coordinates 186435..186722 (+) Length 96 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
BEI49_RS01080 181751..182713 + 963 WP_000961155.1 UV DNA damage repair endonuclease UvsE -
BEI49_RS01085 182706..183278 - 573 WP_000906926.1 rhomboid family intramembrane serine protease -
BEI49_RS01090 183371..183730 + 360 WP_000635040.1 holo-ACP synthase -
BEI49_RS01095 183887..184837 + 951 WP_025388382.1 outer membrane lipoprotein carrier protein LolA -
BEI49_RS01100 184956..186125 + 1170 WP_075396319.1 alanine racemase -
BEI49_RS01105 186435..186722 + 288 WP_000004570.1 antitoxin EndoAI Antitoxin
BEI49_RS01110 186727..187077 + 351 WP_000635963.1 type II toxin-antitoxin system endoribonuclease NdoA Toxin
BEI49_RS01115 187145..189313 + 2169 WP_000426225.1 RNA-binding transcriptional accessory protein -
BEI49_RS01120 189371..189487 - 117 WP_001143642.1 cortex morphogenetic protein CmpA -
BEI49_RS01125 189683..190141 + 459 WP_000344248.1 SprT family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(1-113)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 12974.12 Da        Isoelectric Point: 5.7168

>T70955 WP_000635963.1 NZ_CP018933:186727-187077 [Bacillus cereus]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTVIVAAITAQIQKAKLPTHVEIDAKKYGFERDSVILLEQI
RTIDKQRLTDKITHLDEVMMIRVDEALQISLGLIDF

Download         Length: 351 bp

>T70955 NZ_CP018933:186727-187077 [Bacillus cereus]
TTGATTGTAAAACGCGGCGACGTGTATTTTGCAGACCTTTCCCCAGTTGTTGGTTCTGAGCAAGGAGGCGTTCGTCCGGT
TCTTGTCATTCAAAATGACATCGGAAATCGTTTTAGTCCAACGGTGATTGTAGCGGCTATTACTGCACAGATTCAAAAAG
CGAAATTACCCACTCATGTGGAAATTGATGCGAAAAAGTACGGTTTTGAGAGAGATTCTGTTATTTTACTTGAGCAGATT
CGAACAATCGATAAGCAGCGCTTAACGGACAAAATCACTCACTTAGATGAAGTGATGATGATTCGTGTAGATGAAGCGCT
ACAAATTAGTTTAGGACTAATAGATTTTTAA

Antitoxin


Download         Length: 96 a.a.        Molecular weight: 10780.36 Da        Isoelectric Point: 8.9605

>AT70955 WP_000004570.1 NZ_CP018933:186435-186722 [Bacillus cereus]
VSESSVTTEIVVRLPKQMVTELDGIGKQENKNRHELICQATQLLLRQHKTKKRYQHESMRRGYIEMGKINLGIASEAFLA
EYEAAHTVERLVSGG

Download         Length: 288 bp

>AT70955 NZ_CP018933:186435-186722 [Bacillus cereus]
GTGTCCGAATCAAGTGTAACTACTGAAATCGTGGTTCGGTTGCCAAAGCAAATGGTAACGGAATTGGACGGAATTGGAAA
ACAAGAGAATAAGAATCGCCATGAACTAATTTGCCAGGCAACACAACTGTTATTGCGTCAACATAAGACGAAGAAACGCT
ACCAACATGAATCAATGCGACGTGGGTACATTGAAATGGGAAAAATTAATCTTGGTATTGCATCTGAAGCTTTCTTAGCA
GAGTATGAAGCAGCTCATACAGTAGAACGCTTAGTTAGCGGGGGGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4HKE
PDB 7BXY

Antitoxin

Source ID Structure
AlphaFold DB A0A366FY90

References