Detailed information of TA system TA070952

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/LOTUS_5_Limkain_b1(toxin)
• Location: 4554420..4555021
• Replicon: chromosome
• Accession: NZ_CP018930
• Organism: Serratia marcescens strain UMH12

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: BVG84_RS21735
• Protein ID: WP_060448309.1
• Coordinates: 4554420..4554800 (-)
• Length: 127 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: A0A8B4GSF3
• Locus tag: BVG84_RS21740
• Protein ID: WP_025304767.1
• Coordinates: 4554800..4555021 (-)
• Length: 74 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BVG84_RS21710	4549668..4550948	+	1281	WP_060448306.1	DUF3748 domain-containing protein	-
BVG84_RS21715	4550979..4551326	-	348	WP_016929593.1	YceK/YidQ family lipoprotein	-
BVG84_RS21720	4551636..4552049	+	414	WP_004933919.1	heat shock chaperone IbpA	-
BVG84_RS21725	4552156..4552584	+	429	WP_004933922.1	heat shock chaperone IbpB	-
BVG84_RS21730	4552751..4554409	+	1659	WP_060448308.1	putative transporter	-
BVG84_RS21735	4554420..4554800	-	381	WP_060448309.1	type II toxin-antitoxin system VapC family toxin	Toxin
BVG84_RS21740	4554800..4555021	-	222	WP_025304767.1	ribbon-helix-helix domain-containing protein	Antitoxin
BVG84_RS21745	4555097..4556347	-	1251	WP_049204660.1	valine--pyruvate transaminase	-
BVG84_RS21750	4556483..4558546	-	2064	WP_060448312.1	alpha-amylase	-
BVG84_RS25150	4558743..4558895	+	153	WP_195904408.1	hypothetical protein	-
BVG84_RS21755	4558897..4559874	-	978	WP_049204664.1	glyoxylate/hydroxypyruvate reductase GhrB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (7-100)

Antitoxin

• Ribbon-helix-helix protein, CopG (3-40)

Sequences

Toxin

Protein

• Length: 127 a.a.
• Molecular weight: 14183.62 Da
• Isoelectric Point: 8.5450

>T70952 WP_060448309.1 NZ_CP018930:c4554800-4554420 [Serratia marcescens]
MVAQRALFDTNILIDYLNGIPQARDVLVEYHINPAISAITWMEVMVGAKKQGPALELKTRQFLGQFLLLPITDEVAERAV
ELRHSQHVKLPGAIIWATAQVGFRMLISRNPKDFGTDNGVLMPYRL

Nucleotide

• Length: 381 bp

>T70952 NZ_CP018930:c4554800-4554420 [Serratia marcescens]
ATGGTGGCTCAACGGGCGTTATTCGACACCAATATCCTGATCGATTATCTCAACGGCATTCCTCAAGCCAGGGACGTTCT
GGTCGAGTATCACATCAATCCGGCCATCAGCGCCATCACCTGGATGGAAGTGATGGTAGGTGCTAAAAAACAGGGTCCGG
CACTGGAGCTGAAAACACGTCAGTTTTTAGGGCAATTTCTGCTGCTGCCTATTACCGATGAAGTCGCTGAACGCGCCGTA
GAACTGCGCCACTCACAGCATGTGAAACTGCCAGGCGCCATTATCTGGGCCACAGCGCAGGTGGGATTCCGGATGCTCAT
CTCCCGCAACCCCAAAGATTTCGGCACTGACAATGGCGTGTTGATGCCTTATCGGCTGTAG

Antitoxin

Protein

• Length: 74 a.a.
• Molecular weight: 8607.55 Da
• Isoelectric Point: 4.4915

>AT70952 WP_025304767.1 NZ_CP018930:c4555021-4554800 [Serratia marcescens]
MTRIVIDLPEEDLRQLDNLKNIRHVPRAEIIRQAVSRYLTENHVEDPSNAFGLWSGAMGDGVEYENRQREEWE

Nucleotide

• Length: 222 bp

>AT70952 NZ_CP018930:c4555021-4554800 [Serratia marcescens]
ATGACTCGCATCGTGATCGATTTACCTGAAGAAGACCTGCGGCAGCTCGATAATCTGAAAAATATCCGCCACGTTCCGCG
GGCCGAGATCATTCGTCAAGCAGTCTCCCGCTATCTGACGGAGAACCACGTGGAAGATCCCAGCAACGCTTTCGGGCTCT
GGTCCGGGGCGATGGGAGATGGCGTGGAGTACGAAAATCGTCAGCGCGAGGAGTGGGAATAA

Structures

Antitoxin

• AlphaFold DB: A0A8B4GSF3