Detailed information of TA system TA070866

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): ccdAB/CcdA(antitoxin)
• Location: 898340..898893
• Replicon: chromosome
• Accession: NZ_CP018925
• Organism: Serratia marcescens strain UMH3

Toxin (Protein)

• Gene name: ccdB
• Uniprot ID: A0A2V4FZ31
• Locus tag: BVG91_RS04365
• Protein ID: WP_019454426.1
• Coordinates: 898579..898893 (+)
• Length: 105 a.a.

Antitoxin (Protein)

• Gene name: ccdA
• Uniprot ID: -
• Locus tag: BVG91_RS04360
• Protein ID: WP_039566839.1
• Coordinates: 898340..898576 (+)
• Length: 79 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BVG91_RS04345	894807..896342	+	1536	WP_033646792.1	glutathione ABC transporter substrate-binding protein GsiB	-
BVG91_RS04350	896395..897315	+	921	WP_033646790.1	glutathione ABC transporter permease GsiC	-
BVG91_RS04355	897325..898233	+	909	WP_049233469.1	glutathione ABC transporter permease GsiD	-
BVG91_RS04360	898340..898576	+	237	WP_039566839.1	type II toxin-antitoxin system CcdA family antitoxin	Antitoxin
BVG91_RS04365	898579..898893	+	315	WP_019454426.1	CcdB family protein	Toxin
BVG91_RS04370	898933..899775	-	843	WP_015377214.1	S-formylglutathione hydrolase	-
BVG91_RS04375	899790..900914	-	1125	WP_033646788.1	S-(hydroxymethyl)glutathione dehydrogenase/class III alcohol dehydrogenase	-
BVG91_RS04380	900945..901865	-	921	WP_016928453.1	LysR family transcriptional regulator	-
BVG91_RS04385	901971..903128	+	1158	WP_089187058.1	YbfB/YjiJ family MFS transporter	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin CcdB (2-103)

Antitoxin

• Post-segregation antitoxin CcdA (9-78)

Sequences

Toxin

Protein

• Length: 105 a.a.
• Molecular weight: 11745.68 Da
• Isoelectric Point: 5.0488

>T70866 WP_019454426.1 NZ_CP018925:898579-898893 [Serratia marcescens]
MQFTVYANRGNSAVYPLLLDVTSDIIGQLNRRVVIPLLPVEKYPGSTRPERLIPLIRLIDDNEYAVMTYEMASIPVRALG
AEFCDVSQYRTRIKAAIDFLLDGI

Nucleotide

• Length: 315 bp

>T70866 NZ_CP018925:898579-898893 [Serratia marcescens]
ATGCAATTTACGGTGTATGCGAATAGGGGGAACAGCGCGGTTTATCCCCTGCTGCTTGATGTGACGAGCGATATCATTGG
GCAATTAAATCGTCGAGTTGTGATCCCTTTGTTGCCCGTTGAAAAATACCCGGGGAGTACGCGACCGGAACGCTTGATCC
CGTTGATCAGATTGATTGATGACAACGAGTACGCCGTGATGACATATGAAATGGCCAGCATCCCGGTTCGGGCTTTGGGG
GCCGAGTTTTGTGATGTTTCCCAGTATCGGACCAGGATAAAAGCCGCCATCGACTTTCTTCTTGACGGTATTTGA

Antitoxin

Protein

• Length: 79 a.a.
• Molecular weight: 9068.10 Da
• Isoelectric Point: 5.7070

>AT70866 WP_039566839.1 NZ_CP018925:898340-898576 [Serratia marcescens]
MPVKQRTTQSVTMTVERSLLSRAREAGINLSATLTAALDAELRQHERIKWQEENREALEALNRFNDEHGCFSDEYRTF

Nucleotide

• Length: 237 bp

>AT70866 NZ_CP018925:898340-898576 [Serratia marcescens]
ATGCCAGTAAAACAACGAACGACGCAAAGTGTGACCATGACGGTGGAACGCAGTTTGTTGAGCCGTGCGCGTGAGGCGGG
GATTAATCTCAGCGCGACGCTAACTGCTGCTTTAGATGCTGAGTTACGTCAGCATGAGAGGATAAAATGGCAGGAAGAAA
ACCGAGAGGCGCTTGAAGCGCTTAACCGATTTAATGATGAGCATGGTTGCTTTAGCGACGAATACAGGACGTTTTAG

Structures

Toxin

• AlphaFold DB: A0A2V4FZ31