Detailed information of TA system TA070787

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 3576373..3577010
• Replicon: chromosome
• Accession: NZ_CP018902
• Organism: Bacillus amyloliquefaciens strain HK1

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: G4NU33
• Locus tag: BUN12_RS18570
• Protein ID: WP_003156187.1
• Coordinates: 3576660..3577010 (+)
• Length: 117 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: I2HMS5
• Locus tag: BUN12_RS18565
• Protein ID: WP_003156188.1
• Coordinates: 3576373..3576654 (+)
• Length: 94 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BUN12_RS18545	3572739..3573338	-	600	WP_013351092.1	rhomboid family intramembrane serine protease	-
BUN12_RS18550	3573431..3573796	+	366	WP_013351093.1	holo-ACP synthase	-
BUN12_RS18555	3573961..3574968	+	1008	WP_013351094.1	outer membrane lipoprotein carrier protein LolA	-
BUN12_RS18560	3575085..3576254	+	1170	WP_013351095.1	alanine racemase	-
BUN12_RS18565	3576373..3576654	+	282	WP_003156188.1	type II toxin-antitoxin system antitoxin EndoAI	Antitoxin
BUN12_RS18570	3576660..3577010	+	351	WP_003156187.1	type II toxin-antitoxin system endoribonuclease NdoA	Toxin
BUN12_RS18575	3577131..3577952	+	822	WP_013351096.1	STAS domain-containing protein	-
BUN12_RS18580	3577957..3578322	+	366	WP_003156180.1	RsbT antagonist protein RsbS	-
BUN12_RS18585	3578325..3578726	+	402	WP_013351097.1	anti-sigma regulatory factor	-
BUN12_RS18590	3578738..3579745	+	1008	WP_013351098.1	PP2C family protein-serine/threonine phosphatase	-
BUN12_RS18595	3579809..3580138	+	330	WP_013351099.1	anti-sigma factor antagonist	-
BUN12_RS18600	3580135..3580617	+	483	WP_013351100.1	anti-sigma B factor RsbW	-
BUN12_RS18605	3580583..3581371	+	789	WP_013351101.1	RNA polymerase sigma factor SigB	-
BUN12_RS18610	3581371..3581957	+	587	Protein_3582	SpoIIE family protein phosphatase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (4-111)

Antitoxin

• Ribbon-helix-helix protein, CopG (12-45)

Sequences

Toxin

Protein

• Length: 117 a.a.
• Molecular weight: 12977.98 Da
• Isoelectric Point: 4.8781

>T70787 WP_003156187.1 NZ_CP018902:3576660-3577010 [Bacillus amyloliquefaciens]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTAIVAAITAQIQKAKLPTHVEIDAKRYGFERDSVILLEQI
RTIDKQRLTDKITHLDDEMMDKVDEALQISLALIDF

Nucleotide

• Length: 351 bp

>T70787 NZ_CP018902:3576660-3577010 [Bacillus amyloliquefaciens]
TTGATTGTAAAGCGCGGCGATGTTTATTTTGCTGATTTATCTCCTGTTGTTGGCTCAGAGCAAGGCGGCGTACGCCCGGT
TTTAGTGATCCAAAATGATATCGGAAATCGCTTTAGCCCAACTGCTATTGTTGCAGCCATAACAGCCCAAATACAGAAAG
CGAAATTACCAACCCACGTCGAAATTGATGCAAAACGCTACGGTTTTGAAAGAGATTCCGTTATTTTGCTGGAGCAAATT
CGGACGATTGACAAGCAAAGATTAACGGATAAAATAACTCATCTGGATGATGAAATGATGGATAAGGTTGATGAAGCCCT
GCAAATCAGTTTGGCACTCATTGATTTTTAG

Antitoxin

Protein

• Length: 94 a.a.
• Molecular weight: 10562.92 Da
• Isoelectric Point: 6.4706

>AT70787 WP_003156188.1 NZ_CP018902:3576373-3576654 [Bacillus amyloliquefaciens]
MSESSARTEMKISLPENLVAELDGVAMREKRSRNELISQAVRAYVSERTTRHNRDLMRRGYMEMAKINLNISSEAHYAEC
EAETTVERLVSGG

Nucleotide

• Length: 282 bp

>AT70787 NZ_CP018902:3576373-3576654 [Bacillus amyloliquefaciens]
TTGTCTGAATCCAGCGCAAGAACCGAAATGAAAATCAGCTTGCCTGAAAACTTAGTAGCTGAATTGGATGGTGTAGCGAT
GCGGGAGAAACGAAGCAGAAACGAACTGATATCACAGGCAGTGAGAGCGTATGTCAGCGAACGGACAACTCGCCATAACC
GTGATTTGATGAGACGCGGGTATATGGAAATGGCGAAAATCAACTTGAATATTTCTTCTGAGGCTCACTATGCTGAGTGC
GAAGCAGAAACGACGGTTGAGCGCTTAGTCAGCGGAGGATAA

Structures

Toxin

• PDB: 4MDX
• PDB: 4ME7
• PDB: 1NE8

Antitoxin

• AlphaFold DB: I2HMS5