Detailed information of TA system TA070778

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): PP_4151-4152/excise(antitoxin)
• Location: 496873..497894
• Replicon: chromosome
• Accession: NZ_CP018889
• Organism: Beggiatoa leptomitoformis strain D-401

Toxin (Protein)

• Gene name: PP_4152
• Uniprot ID: A0A2N9YBR8
• Locus tag: BLE401_RS02135
• Protein ID: WP_062148331.1
• Coordinates: 496873..497439 (-)
• Length: 189 a.a.

Antitoxin (Protein)

• Gene name: PP_4151
• Uniprot ID: -
• Locus tag: BLE401_RS02140
• Protein ID: WP_101539118.1
• Coordinates: 497439..497894 (-)
• Length: 152 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BLE401_RS02120	492151..494337	+	2187	WP_062148320.1	hypothetical protein	-
BLE401_RS02125	494418..495593	-	1176	WP_062148325.1	pyridoxal phosphate-dependent aminotransferase	-
BLE401_RS02130	495738..496751	-	1014	WP_062148328.1	type I glyceraldehyde-3-phosphate dehydrogenase	-
BLE401_RS02135	496873..497439	-	567	WP_062148331.1	PIN domain-containing protein	Toxin
BLE401_RS02140	497439..497894	-	456	WP_101539118.1	helix-turn-helix domain-containing protein	Antitoxin
BLE401_RS02145	498056..500047	-	1992	WP_062148337.1	transketolase	-
BLE401_RS02150	500394..501038	+	645	WP_062155304.1	orotate phosphoribosyltransferase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (6-112)

Antitoxin

• SinI-like, DNA-binding domain (80-126)

Sequences

Toxin

Protein

• Length: 189 a.a.
• Molecular weight: 21778.11 Da
• Isoelectric Point: 5.6425

>T70778 WP_062148331.1 NZ_CP018889:c497439-496873 [Beggiatoa leptomitoformis]
MASFSVICDACVLYPAPLRDLLMRLALADLFKAHWTDKIHEEWINALLRQNKYKRDELEKVRDLMDKHIKDAKVDGYETL
INGLSLPDENDRHVLAAAIKINADAIITFNTKDFPSDVLNQYAIEVIHPDDFIFYQLELNSALCFQAIKEQRQTLKNPPK
TAEEFLRILQKQSLQQTVSKLAEYINFI

Nucleotide

• Length: 567 bp

>T70778 NZ_CP018889:c497439-496873 [Beggiatoa leptomitoformis]
ATGGCAAGTTTCTCAGTCATTTGCGATGCTTGTGTTTTATACCCTGCACCGTTGCGGGATTTACTCATGCGATTAGCATT
AGCCGATTTATTCAAAGCCCATTGGACAGATAAAATTCACGAAGAATGGATAAATGCGCTGCTACGACAAAATAAATATA
AACGAGATGAATTAGAAAAAGTTCGCGATTTAATGGACAAACATATAAAAGATGCAAAAGTAGATGGTTATGAAACATTA
ATAAATGGCTTATCTTTACCTGATGAAAATGATAGGCACGTTTTAGCGGCTGCTATCAAAATAAATGCAGATGCAATTAT
TACATTTAATACAAAGGATTTTCCTTCAGATGTTTTAAATCAATATGCAATTGAGGTTATTCACCCTGATGATTTTATTT
TTTATCAACTTGAATTAAACTCCGCTTTATGTTTTCAAGCCATTAAAGAACAACGACAAACCCTTAAAAATCCACCTAAA
ACGGCTGAAGAGTTTTTACGTATTTTACAAAAACAATCTTTACAACAAACGGTTTCAAAATTGGCAGAATATATAAATTT
TATTTAA

Antitoxin

Protein

• Length: 152 a.a.
• Molecular weight: 16911.62 Da
• Isoelectric Point: 7.3990

>AT70778 WP_101539118.1 NZ_CP018889:c497894-497439 [Beggiatoa leptomitoformis]
MMSAINLVKLPTYQESEQAKSSSRVLAKYIANGRLQLSIKADDSQEELILPNYAIDLLLNVLTEMAKGNAITLMPIHAEL
TTQEAAELLNVSRPHLVSLLEKGEIPFHKIGSHRRVLAKDIIEYKKNLRTNRLQALNELTTLSQTLGMGYE

Nucleotide

• Length: 456 bp

>AT70778 NZ_CP018889:c497894-497439 [Beggiatoa leptomitoformis]
ATGATGAGTGCAATTAATTTGGTAAAGCTACCGACCTATCAAGAAAGTGAACAAGCGAAAAGTAGTAGCCGTGTGTTGGC
AAAATATATTGCTAATGGTCGATTACAACTCAGCATTAAAGCTGATGATTCACAAGAAGAATTAATTTTACCCAATTACG
CAATCGACTTACTGCTTAATGTGTTGACTGAAATGGCGAAAGGTAATGCAATCACCCTAATGCCTATTCATGCTGAATTG
ACGACTCAAGAAGCCGCTGAATTATTAAACGTCAGTCGTCCCCATTTAGTCAGCCTATTAGAAAAAGGAGAAATCCCTTT
TCATAAAATTGGTTCACATCGTCGTGTTCTAGCAAAGGATATCATTGAATACAAAAAAAATTTAAGAACCAATCGCTTAC
AAGCACTTAATGAATTAACTACCCTCTCCCAAACACTCGGCATGGGATATGAGTGA

Structures

Toxin

• AlphaFold DB: A0A2N9YBR8