Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 30548..31191 Replicon plasmid pIncN
Accession NZ_CP018887
Organism Klebsiella pneumoniae subsp. pneumoniae strain BR21

Toxin (Protein)


Gene name vagD Uniprot ID A0A5Q9LNG2
Locus tag BU813_RS28805 Protein ID WP_048983057.1
Coordinates 30548..30964 (-) Length 139 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID Q84A07
Locus tag BU813_RS28810 Protein ID WP_001261276.1
Coordinates 30961..31191 (-) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
BU813_RS28790 25596..27704 - 2109 WP_016338368.1 peptidase domain-containing ABC transporter -
BU813_RS28795 27694..28971 - 1278 WP_016338369.1 HlyD family secretion protein -
BU813_RS28800 29630..30507 + 878 Protein_35 restriction endonuclease -
BU813_RS28805 30548..30964 - 417 WP_048983057.1 type II toxin-antitoxin system VapC family toxin Toxin
BU813_RS28810 30961..31191 - 231 WP_001261276.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein Antitoxin
BU813_RS28825 32106..32414 + 309 WP_004152336.1 hypothetical protein -
BU813_RS28830 32442..32771 + 330 WP_004152337.1 hypothetical protein -
BU813_RS28835 32797..33195 + 399 WP_004171440.1 hypothetical protein -
BU813_RS28840 33202..33534 + 333 WP_004152339.1 hypothetical protein -
BU813_RS28845 33534..34316 + 783 WP_004152340.1 site-specific integrase -
BU813_RS28850 35208..35438 - 231 WP_011977773.1 hypothetical protein -
BU813_RS28855 35530..36003 - 474 WP_004152341.1 YkgJ family cysteine cluster protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid dfrA30 / tet(A) / blaOXA-1 / aac(6')-Ib-cr / blaKPC-2 - 1..123208 123208
- flank IS/Tn - - 36123..37391 1268


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(6-129)

Antitoxin

SpoVT-AbrB domain

(7-47)


Sequences

Toxin        


Download         Length: 139 a.a.        Molecular weight: 15022.52 Da        Isoelectric Point: 9.2957

>T70775 WP_048983057.1 NZ_CP018887:c30964-30548 [Klebsiella pneumoniae subsp. pneumoniae]
MKKTWMLDTNICSFIMREQPAAVLKRLEQAVLRGDRIVVSAVTYAEMRFGATGPKASPRHIQLVDAFCARLDAILPWDRA
AVDATTDIRVALRLAGTPIGPNDTAIAGHAIAAGAILVTNNTREFARVPGLVLEDWVK

Download         Length: 417 bp

>T70775 NZ_CP018887:c30964-30548 [Klebsiella pneumoniae subsp. pneumoniae]
ATGAAGAAAACCTGGATGCTCGACACTAACATCTGCTCGTTCATCATGCGCGAGCAGCCGGCAGCGGTGCTGAAGCGCCT
GGAGCAGGCGGTGCTGCGCGGTGATCGCATCGTGGTCTCGGCCGTGACGTATGCCGAGATGCGCTTCGGCGCCACCGGCC
CGAAGGCCTCGCCGCGCCATATTCAGCTGGTCGACGCGTTCTGCGCGCGCCTCGATGCCATCCTGCCCTGGGACCGGGCC
GCGGTGGACGCCACGACGGACATCCGGGTGGCGCTGCGCCTCGCCGGGACGCCGATCGGCCCGAACGACACGGCCATTGC
CGGCCACGCCATCGCGGCCGGCGCCATCCTGGTGACGAATAATACAAGAGAGTTTGCGCGGGTCCCGGGCCTGGTGCTGG
AAGACTGGGTGAAGTAA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8721.74 Da        Isoelectric Point: 4.3533

>AT70775 WP_001261276.1 NZ_CP018887:c31191-30961 [Klebsiella pneumoniae subsp. pneumoniae]
MRTVSIFKNGNNRAIRLPRDLDFDGVSELEIVREGDSIILRPVRPTWGSFAQLDRADPDFMAEREDVVSDEGRFEP

Download         Length: 231 bp

>AT70775 NZ_CP018887:c31191-30961 [Klebsiella pneumoniae subsp. pneumoniae]
ATGCGCACAGTTTCGATATTTAAAAACGGCAATAACCGCGCCATCCGTCTGCCCCGGGACCTGGATTTTGACGGCGTCAG
CGAGCTGGAGATCGTCCGGGAAGGGGACAGCATCATTCTGCGTCCCGTCCGGCCGACCTGGGGCTCGTTCGCGCAGCTGG
ACAGGGCCGATCCGGACTTTATGGCGGAGCGTGAGGATGTGGTCAGCGATGAAGGACGCTTTGAGCCATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A5Q9LNG2

Antitoxin

Source ID Structure
AlphaFold DB A0A387K023

References