Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tacAT/DUF1778(antitoxin)
Location 63329..64065 Replicon plasmid pIncF
Accession NZ_CP018886
Organism Klebsiella pneumoniae subsp. pneumoniae strain BR21

Toxin (Protein)


Gene name tacT Uniprot ID L7SZ15
Locus tag BU813_RS27675 Protein ID WP_003026803.1
Coordinates 63583..64065 (+) Length 161 a.a.

Antitoxin (Protein)


Gene name tacA Uniprot ID L7SZ29
Locus tag BU813_RS27670 Protein ID WP_003026799.1
Coordinates 63329..63595 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
BU813_RS27625 59391..59753 - 363 WP_004152100.1 arsenite efflux transporter metallochaperone ArsD -
BU813_RS27630 59803..60153 - 351 WP_004152101.1 As(III)-sensing metalloregulatory transcriptional repressor ArsR -
BU813_RS27635 60511..60780 + 270 WP_004152102.1 hypothetical protein -
BU813_RS27640 60768..61343 + 576 WP_004152103.1 hypothetical protein -
BU813_RS27645 61374..61868 + 495 WP_004152104.1 DNA-binding protein -
BU813_RS27650 61912..62280 + 369 WP_004152105.1 hypothetical protein -
BU813_RS27655 62314..62517 + 204 WP_004152106.1 hemolysin expression modulator Hha -
BU813_RS27660 62566..62823 + 258 WP_004152107.1 hypothetical protein -
BU813_RS27665 62899..63153 + 255 WP_004152108.1 hypothetical protein -
BU813_RS27670 63329..63595 + 267 WP_003026799.1 DUF1778 domain-containing protein Antitoxin
BU813_RS27675 63583..64065 + 483 WP_003026803.1 GNAT family N-acetyltransferase Toxin
BU813_RS27680 64277..65623 + 1347 WP_020314316.1 ISNCY family transposase -
BU813_RS27685 66124..67378 - 1255 Protein_66 IS3 family transposase -
BU813_RS27690 67466..68428 - 963 WP_004152113.1 M48 family metalloprotease -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid aph(3')-Ia / mph(A) / sul1 / qacE - 1..217456 217456


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(45-149)

Antitoxin

Vibrio phage ICP1, Orf50

(6-82)


Sequences

Toxin        


Download         Length: 161 a.a.        Molecular weight: 17353.97 Da        Isoelectric Point: 9.5822

>T70774 WP_003026803.1 NZ_CP018886:63583-64065 [Klebsiella pneumoniae subsp. pneumoniae]
VGRITTPEPLSSSHQLAEFVSGETVLDEWLKQRGLKNQALGAARTFVVCKTGTKQVAGFYSLATGSVNHTEATGSLRRNM
PDPIPVIILARLAVDVSLHGKGVGADLLHDAVLRCYRVAENIGVRAIMVHALTEEAKGFYAHHGFKASQTHERTLFLKLP

Download         Length: 483 bp

>T70774 NZ_CP018886:63583-64065 [Klebsiella pneumoniae subsp. pneumoniae]
GTGGGACGTATAACCACGCCCGAGCCGTTATCCAGCTCTCATCAGCTGGCTGAGTTCGTCAGTGGAGAGACAGTCCTCGA
TGAATGGCTAAAACAGAGAGGTTTAAAAAATCAAGCTCTTGGCGCTGCCCGAACGTTCGTTGTTTGCAAGACGGGTACGA
AGCAAGTAGCTGGTTTTTACTCTTTGGCCACCGGTAGCGTTAACCATACGGAAGCGACAGGTAGTCTTCGGCGTAACATG
CCAGATCCTATACCGGTGATTATACTCGCTCGCCTGGCGGTAGATGTCTCATTGCACGGAAAAGGGGTTGGCGCCGATTT
ACTCCACGATGCAGTTCTACGGTGTTATCGCGTAGCTGAGAACATTGGGGTTCGTGCGATAATGGTTCATGCGCTTACTG
AAGAAGCCAAAGGTTTCTATGCTCACCATGGATTTAAGGCATCACAAACTCATGAGAGGACTCTGTTTCTAAAGCTTCCA
TGA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10147.59 Da        Isoelectric Point: 4.5256

>AT70774 WP_003026799.1 NZ_CP018886:63329-63595 [Klebsiella pneumoniae subsp. pneumoniae]
MKSDVQLNLRAKESQRALIDAAAEILHKSRTDFILEMACKAAENVILDRRVFNFNDEQYAEFIDMLDAPVEDEPAINKLL
ARKPQWDV

Download         Length: 267 bp

>AT70774 NZ_CP018886:63329-63595 [Klebsiella pneumoniae subsp. pneumoniae]
ATGAAATCAGATGTTCAACTCAACCTAAGAGCTAAAGAGTCTCAGAGAGCACTCATCGATGCTGCTGCAGAGATCCTTCA
TAAATCGCGAACCGACTTTATCCTGGAGATGGCCTGCAAGGCTGCGGAGAATGTGATCCTTGATCGCCGTGTTTTCAATT
TTAACGACGAACAGTATGCAGAGTTCATCGATATGCTCGATGCACCAGTCGAGGATGAGCCCGCCATTAATAAACTACTG
GCAAGGAAACCTCAGTGGGACGTATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0J2GNW6

Antitoxin

Source ID Structure
AlphaFold DB A0A1Q8YL66

References