Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 2718081..2718700 Replicon chromosome
Accession NZ_CP018885
Organism Klebsiella pneumoniae subsp. pneumoniae strain BR21

Toxin (Protein)


Gene name Hha Uniprot ID R8WYV2
Locus tag BU813_RS13940 Protein ID WP_002892050.1
Coordinates 2718081..2718299 (-) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID J2DPF6
Locus tag BU813_RS13945 Protein ID WP_002892066.1
Coordinates 2718326..2718700 (-) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
BU813_RS13910 2714128..2714391 + 264 WP_002892011.1 type B 50S ribosomal protein L31 -
BU813_RS13915 2714391..2714531 + 141 WP_002892018.1 type B 50S ribosomal protein L36 -
BU813_RS13920 2714528..2715226 - 699 WP_002892021.1 GNAT family N-acetyltransferase -
BU813_RS13925 2715327..2716778 + 1452 WP_002892023.1 PLP-dependent aminotransferase family protein -
BU813_RS13930 2716753..2717223 - 471 WP_002892026.1 YlaC family protein -
BU813_RS29780 2717244..2717384 + 141 WP_004147370.1 hypothetical protein -
BU813_RS13935 2717356..2717922 - 567 WP_002892030.1 maltose O-acetyltransferase -
BU813_RS13940 2718081..2718299 - 219 WP_002892050.1 hemolysin expression modulator Hha Toxin
BU813_RS13945 2718326..2718700 - 375 WP_002892066.1 Hha toxicity modulator TomB Antitoxin
BU813_RS13950 2719186..2722332 - 3147 WP_002892069.1 multidrug efflux RND transporter permease subunit AcrB -
BU813_RS13955 2722355..2723548 - 1194 WP_002892072.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-116)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T70764 WP_002892050.1 NZ_CP018885:c2718299-2718081 [Klebsiella pneumoniae subsp. pneumoniae]
MSDKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPTSVWKFIR

Download         Length: 219 bp

>T70764 NZ_CP018885:c2718299-2718081 [Klebsiella pneumoniae subsp. pneumoniae]
ATGTCTGATAAGCCATTAACTAAAACAGACTATTTGATGCGCTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGCGT
CATTGAAAAAAATAAATATGAACTGTCTGATAATGAGCTGGCGGTATTTTACTCAGCTGCCGACCATCGTCTGGCGGAAC
TGACCATGAATAAGCTATACGATAAAATCCCGACTTCTGTCTGGAAATTTATCCGCTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14368.06 Da        Isoelectric Point: 4.8989

>AT70764 WP_002892066.1 NZ_CP018885:c2718700-2718326 [Klebsiella pneumoniae subsp. pneumoniae]
MDEYSPKRHDIAQLRFLCETLYHDCLANLEESNHGWVNDPTSAVNLQLNELIEHIATFALNYKIKYAEDNKLIAQVDEYL
DDTFTLFSNYGINSTDLQKWKKSGNRLFRCFVNASRENPASLSC

Download         Length: 375 bp

>AT70764 NZ_CP018885:c2718700-2718326 [Klebsiella pneumoniae subsp. pneumoniae]
ATGGACGAATACTCGCCAAAAAGACATGATATTGCACAGCTACGCTTTCTCTGCGAAACGCTGTATCATGACTGCCTTGC
AAACCTGGAAGAGAGCAACCATGGCTGGGTCAACGACCCGACGTCGGCTGTCAATCTCCAGTTAAATGAATTGATCGAGC
ACATTGCCACATTCGCTCTTAATTATAAAATTAAGTATGCAGAGGATAATAAGCTGATCGCTCAGGTTGACGAATATCTG
GACGACACTTTTACGTTGTTCAGTAACTACGGCATTAACTCTACGGATTTGCAAAAGTGGAAAAAATCCGGTAACCGACT
ATTTCGTTGCTTTGTCAACGCCAGCCGGGAAAACCCGGCCAGTCTATCGTGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2P8K6F2

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GJ93

References