Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 8511..9154 Replicon plasmid unnamed1
Accession NZ_CP018884
Organism Klebsiella pneumoniae subsp. pneumoniae strain BR7

Toxin (Protein)


Gene name vagD Uniprot ID A0A5Q9LNG2
Locus tag BU195_RS26985 Protein ID WP_048983057.1
Coordinates 8738..9154 (+) Length 139 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID Q84A07
Locus tag BU195_RS26980 Protein ID WP_001261276.1
Coordinates 8511..8741 (+) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
BU195_RS26935 3699..4172 + 474 WP_004152341.1 YkgJ family cysteine cluster protein -
BU195_RS26940 4264..4494 + 231 WP_011977773.1 hypothetical protein -
BU195_RS26945 5386..6168 - 783 WP_004152340.1 site-specific integrase -
BU195_RS26950 6168..6500 - 333 WP_004152339.1 hypothetical protein -
BU195_RS26955 6507..6905 - 399 WP_004171440.1 hypothetical protein -
BU195_RS26960 6931..7260 - 330 WP_004152337.1 hypothetical protein -
BU195_RS26965 7288..7596 - 309 WP_004152336.1 hypothetical protein -
BU195_RS26980 8511..8741 + 231 WP_001261276.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein Antitoxin
BU195_RS26985 8738..9154 + 417 WP_048983057.1 type II toxin-antitoxin system VapC family toxin Toxin
BU195_RS26990 9195..10072 - 878 Protein_12 restriction endonuclease -
BU195_RS26995 10731..12008 + 1278 WP_016338369.1 HlyD family secretion protein -
BU195_RS27000 11998..14106 + 2109 WP_016338368.1 peptidase domain-containing ABC transporter -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid catA1 / aac(3)-IId / blaTEM-1B / blaCTX-M-15 / blaKPC-2 / qnrB1 / qacE / dfrA30 - 1..121863 121863


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(6-129)

Antitoxin

SpoVT-AbrB domain

(7-47)


Sequences

Toxin        


Download         Length: 139 a.a.        Molecular weight: 15022.52 Da        Isoelectric Point: 9.2957

>T70754 WP_048983057.1 NZ_CP018884:8738-9154 [Klebsiella pneumoniae subsp. pneumoniae]
MKKTWMLDTNICSFIMREQPAAVLKRLEQAVLRGDRIVVSAVTYAEMRFGATGPKASPRHIQLVDAFCARLDAILPWDRA
AVDATTDIRVALRLAGTPIGPNDTAIAGHAIAAGAILVTNNTREFARVPGLVLEDWVK

Download         Length: 417 bp

>T70754 NZ_CP018884:8738-9154 [Klebsiella pneumoniae subsp. pneumoniae]
ATGAAGAAAACCTGGATGCTCGACACTAACATCTGCTCGTTCATCATGCGCGAGCAGCCGGCAGCGGTGCTGAAGCGCCT
GGAGCAGGCGGTGCTGCGCGGTGATCGCATCGTGGTCTCGGCCGTGACGTATGCCGAGATGCGCTTCGGCGCCACCGGCC
CGAAGGCCTCGCCGCGCCATATTCAGCTGGTCGACGCGTTCTGCGCGCGCCTCGATGCCATCCTGCCCTGGGACCGGGCC
GCGGTGGACGCCACGACGGACATCCGGGTGGCGCTGCGCCTCGCCGGGACGCCGATCGGCCCGAACGACACGGCCATTGC
CGGCCACGCCATCGCGGCCGGCGCCATCCTGGTGACGAATAATACAAGAGAGTTTGCGCGGGTCCCGGGCCTGGTGCTGG
AAGACTGGGTGAAGTAA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8721.74 Da        Isoelectric Point: 4.3533

>AT70754 WP_001261276.1 NZ_CP018884:8511-8741 [Klebsiella pneumoniae subsp. pneumoniae]
MRTVSIFKNGNNRAIRLPRDLDFDGVSELEIVREGDSIILRPVRPTWGSFAQLDRADPDFMAEREDVVSDEGRFEP

Download         Length: 231 bp

>AT70754 NZ_CP018884:8511-8741 [Klebsiella pneumoniae subsp. pneumoniae]
ATGCGCACAGTTTCGATATTTAAAAACGGCAATAACCGCGCCATCCGTCTGCCCCGGGACCTGGATTTTGACGGCGTCAG
CGAGCTGGAGATCGTCCGGGAAGGGGACAGCATCATTCTGCGTCCCGTCCGGCCGACCTGGGGCTCGTTCGCGCAGCTGG
ACAGGGCCGATCCGGACTTTATGGCGGAGCGTGAGGATGTGGTCAGCGATGAAGGACGCTTTGAGCCATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A5Q9LNG2

Antitoxin

Source ID Structure
AlphaFold DB A0A387K023

References