70728

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazF-pemI/MazF(toxin)
Location 4816075..4816711 Replicon chromosome
Accession NZ_CP018866
Organism Bacillus cohnii strain DSM 6307

Toxin (Protein)


Gene name mazF Uniprot ID A0A223KXJ3
Locus tag BC6307_RS24180 Protein ID WP_066417781.1
Coordinates 4816361..4816711 (+) Length 117 a.a.

Antitoxin (Protein)


Gene name pemI Uniprot ID A0A223KXF9
Locus tag BC6307_RS24175 Protein ID WP_066417778.1
Coordinates 4816075..4816356 (+) Length 94 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
BC6307_RS24155 4812265..4812990 - 726 WP_066417763.1 rhomboid family intramembrane serine protease -
BC6307_RS24160 4813065..4813424 + 360 WP_066417765.1 holo-ACP synthase -
BC6307_RS24165 4813596..4814615 + 1020 WP_174522378.1 outer membrane lipoprotein carrier protein LolA -
BC6307_RS24170 4814768..4815928 + 1161 WP_066417774.1 alanine racemase -
BC6307_RS24175 4816075..4816356 + 282 WP_066417778.1 antitoxin endoai Antitoxin
BC6307_RS24180 4816361..4816711 + 351 WP_066417781.1 type II toxin-antitoxin system endoribonuclease NdoA Toxin
BC6307_RS24185 4817004..4817831 + 828 WP_066417784.1 STAS domain-containing protein -
BC6307_RS24190 4817828..4818184 + 357 WP_066417793.1 STAS domain-containing protein -
BC6307_RS24195 4818189..4818590 + 402 WP_066417794.1 anti-sigma regulatory factor -
BC6307_RS24200 4818607..4819611 + 1005 WP_066417796.1 PP2C family protein-serine/threonine phosphatase -
BC6307_RS24205 4819722..4820030 + 309 WP_066417801.1 STAS domain-containing protein -
BC6307_RS24210 4820053..4820529 + 477 WP_066417802.1 anti-sigma B factor RsbW -
BC6307_RS24215 4820504..4821289 + 786 WP_066417803.1 RNA polymerase sigma factor SigB -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(1-113)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 13048.07 Da        Isoelectric Point: 4.8891

>T70728 WP_066417781.1 NZ_CP018866:4816361-4816711 [Bacillus cohnii]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTIIVAAITAQIQKAKLPTHVEIDAKRYGFERDSVILLEQI
RTIDKQRLTDKITHLDEEMMDRVDEALQISLGLIDF

Download         Length: 351 bp

>T70728 NZ_CP018866:4816361-4816711 [Bacillus cohnii]
TTGATTGTCAAACGCGGTGACGTTTATTTTGCTGATTTATCCCCTGTAGTTGGGTCTGAGCAAGGAGGCGTTCGACCAGT
TCTGGTTATACAAAACGATATTGGTAACCGGTTTAGTCCGACCATTATCGTAGCAGCCATAACAGCACAGATTCAAAAAG
CTAAACTCCCGACACATGTCGAGATTGATGCGAAACGCTATGGCTTTGAACGTGACTCTGTAATATTGTTAGAACAGATT
CGAACCATTGATAAACAAAGATTAACGGACAAAATCACTCATCTTGATGAAGAGATGATGGATCGAGTTGACGAGGCTTT
ACAAATAAGTTTAGGTCTTATTGATTTTTAA

Antitoxin


Download         Length: 94 a.a.        Molecular weight: 10721.22 Da        Isoelectric Point: 7.0152

>AT70728 WP_066417778.1 NZ_CP018866:4816075-4816356 [Bacillus cohnii]
VSESSATTEIMVRLPQNLVSELDGLVKQENGNRNELIYQATKMYLRERKKRQIRESMRRGYMEMAKINLNIASEAFLAEY
EAEHTVERLVSGG

Download         Length: 282 bp

>AT70728 NZ_CP018866:4816075-4816356 [Bacillus cohnii]
GTGTCAGAATCCAGCGCAACAACTGAAATTATGGTTCGATTACCTCAAAACTTAGTATCCGAATTAGATGGATTAGTGAA
ACAGGAAAACGGAAATCGAAATGAGTTAATTTATCAAGCTACAAAAATGTATCTTCGAGAGCGCAAAAAGCGACAAATAC
GTGAATCTATGAGACGTGGCTATATGGAAATGGCAAAGATCAACCTAAATATTGCATCAGAAGCATTTCTAGCAGAATAT
GAGGCAGAGCATACAGTTGAGCGCTTAGTTAGCGGGGGGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A223KXJ3

Antitoxin

Source ID Structure
AlphaFold DB A0A223KXF9

References