Detailed information of TA system TA070725

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/PemK-MazE
• Location: 69146..69687
• Replicon: plasmid pLH201.2
• Accession: NZ_CP018860
• Organism: Xanthomonas citri pv. citri strain LH201

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: BJD09_RS24810
• Protein ID: WP_173667216.1
• Coordinates: 69146..69472 (-)
• Length: 109 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: H8FMA7
• Locus tag: BJD09_RS24815
• Protein ID: WP_003491150.1
• Coordinates: 69469..69687 (-)
• Length: 73 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BJD09_RS24800	64529..66103	+	1575	WP_011052977.1	type IV secretion system DNA-binding domain-containing protein	-
BJD09_RS24805	66117..69092	+	2976	WP_011052978.1	conjugative relaxase	-
BJD09_RS24810	69146..69472	-	327	WP_173667216.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
BJD09_RS24815	69469..69687	-	219	WP_003491150.1	antitoxin MazE family protein	Antitoxin
BJD09_RS24820	69824..70210	-	387	WP_003491149.1	hypothetical protein	-
BJD09_RS24825	70287..70706	-	420	WP_011052981.1	hypothetical protein	-
BJD09_RS24830	70832..71710	-	879	WP_011052982.1	transglycosylase SLT domain-containing protein	-
BJD09_RS24835	71721..72743	-	1023	WP_011052983.1	P-type DNA transfer ATPase VirB11	-
BJD09_RS24840	72754..73974	-	1221	WP_011052984.1	TrbI/VirB10 family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..92707	92707

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (3-103)

Antitoxin

• Antitoxin MazE-like (5-70)

Sequences

Toxin

Protein

• Length: 109 a.a.
• Molecular weight: 11677.64 Da
• Isoelectric Point: 7.1664

>T70725 WP_173667216.1 NZ_CP018860:c69472-69146 [Xanthomonas citri pv. citri]
MMRGDFVTIAMQGDFGEPRPALVIQADQFDAHTTVTVLPVTSTLVAAPLLRITVHPSTDNGLQKPSQVMVDKAMTVKRDK
VGRAFGRVDADALVEIERCLAVFLGIAK

Nucleotide

• Length: 327 bp

>T70725 NZ_CP018860:c69472-69146 [Xanthomonas citri pv. citri]
ATGATGCGGGGTGACTTTGTGACCATCGCCATGCAAGGCGACTTCGGAGAACCAAGGCCCGCTCTGGTGATCCAGGCCGA
CCAGTTTGACGCACACACCACCGTGACGGTGCTGCCGGTGACAAGCACGCTCGTTGCTGCGCCGCTGCTGCGTATTACCG
TTCATCCAAGTACCGACAATGGTTTGCAGAAGCCGTCTCAGGTGATGGTGGACAAGGCCATGACCGTGAAGCGTGACAAG
GTAGGTCGAGCCTTTGGGCGCGTAGACGCTGATGCGTTGGTGGAAATTGAGCGCTGCCTGGCCGTGTTCTTGGGCATCGC
TAAGTGA

Antitoxin

Protein

• Length: 73 a.a.
• Molecular weight: 8418.54 Da
• Isoelectric Point: 5.6599

>AT70725 WP_003491150.1 NZ_CP018860:c69687-69469 [Xanthomonas citri pv. citri]
MAHVNSRVQKHRDALRMAGLRPVQIWVPDTRRPDFAEECRRQCRLAAQADMADTDMQRFMDEALADMDGWTE

Nucleotide

• Length: 219 bp

>AT70725 NZ_CP018860:c69687-69469 [Xanthomonas citri pv. citri]
ATGGCACACGTCAATTCACGAGTTCAAAAGCACCGCGACGCCCTGCGCATGGCAGGGCTGCGTCCGGTGCAAATTTGGGT
GCCGGACACACGGCGGCCTGACTTCGCCGAGGAATGCCGCCGTCAGTGTCGTCTTGCTGCACAAGCGGACATGGCGGATA
CCGACATGCAGCGCTTCATGGATGAGGCGCTAGCAGACATGGATGGCTGGACGGAATGA

Structures

Antitoxin

• AlphaFold DB: H8FMA7