Detailed information of TA system TA070724

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): pemIK/PRK09812-MazE
• Location: 6567..7156
• Replicon: plasmid pLH201.2
• Accession: NZ_CP018860
• Organism: Xanthomonas citri pv. citri strain LH201

Toxin (Protein)

• Gene name: pemK
• Uniprot ID: M4U123
• Locus tag: BJD09_RS24465
• Protein ID: WP_015463824.1
• Coordinates: 6824..7156 (+)
• Length: 111 a.a.

Antitoxin (Protein)

• Gene name: pemI
• Uniprot ID: M4UHU6
• Locus tag: BJD09_RS24460
• Protein ID: WP_011052949.1
• Coordinates: 6567..6824 (+)
• Length: 86 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BJD09_RS26010	2995..3177	+	183	Protein_1	UPF0149 family protein	-
BJD09_RS24435	3251..4465	+	1215	WP_011050083.1	IS4-like element ISXac1 family transposase	-
BJD09_RS24440	4484..5053	+	570	Protein_3	UPF0149 family protein	-
BJD09_RS24445	5098..5304	+	207	Protein_4	Tn3 family transposase	-
BJD09_RS24450	5471..5775	-	305	Protein_5	Tn3 family transposase	-
BJD09_RS24455	5759..6361	-	603	WP_011052950.1	recombinase family protein	-
BJD09_RS24460	6567..6824	+	258	WP_011052949.1	antitoxin	Antitoxin
BJD09_RS24465	6824..7156	+	333	WP_015463824.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
BJD09_RS26015	7179..7430	+	252	WP_125459571.1	hypothetical protein	-
BJD09_RS24470	7427..7639	+	213	WP_011052947.1	hypothetical protein	-
BJD09_RS24475	7629..8165	+	537	WP_015463823.1	plasmid pRiA4b ORF-3 family protein	-
BJD09_RS26260	8455..8676	+	222	WP_011052944.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..92707	92707

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (3-108)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 111 a.a.
• Molecular weight: 11707.46 Da
• Isoelectric Point: 9.1975

>T70724 WP_015463824.1 NZ_CP018860:6824-7156 [Xanthomonas citri pv. citri]
MDRGDVYLVSLDPTSGHEQQGTRPVLIVSPSAFNRLTKTPVVLPITSGGNFARTAGFTVSLMGAGTNTTGVVRCDQPRAL
DLASRRARKLETVPSPIMDEVLAKLSAILE

Nucleotide

• Length: 333 bp

>T70724 NZ_CP018860:6824-7156 [Xanthomonas citri pv. citri]
ATGGATCGCGGCGATGTCTATTTGGTTTCGCTCGATCCCACGTCCGGCCATGAACAACAGGGCACGCGCCCGGTGCTGAT
TGTGTCGCCAAGCGCGTTCAACCGCCTGACGAAAACACCGGTCGTGCTGCCGATCACCAGTGGGGGTAACTTCGCCCGCA
CGGCAGGCTTTACCGTGTCGCTCATGGGTGCGGGTACCAACACAACTGGCGTAGTTCGCTGCGATCAGCCCCGCGCTCTC
GATCTAGCCTCGCGGCGCGCGCGCAAGCTGGAAACCGTGCCGTCCCCGATCATGGATGAGGTATTAGCGAAGCTGAGCGC
GATCCTTGAGTAA

Antitoxin

Protein

• Length: 86 a.a.
• Molecular weight: 9242.70 Da
• Isoelectric Point: 4.7164

>AT70724 WP_011052949.1 NZ_CP018860:6567-6824 [Xanthomonas citri pv. citri]
MHTTNLRKVGGSIMLAVPPAILDMLRLRAGATVGLAVDHGRLVIEPTLRPYYSLDELLAQCDASAELSTEDREWLDAKPV
GSELL

Nucleotide

• Length: 258 bp

>AT70724 NZ_CP018860:6567-6824 [Xanthomonas citri pv. citri]
ATGCATACGACCAATCTGCGTAAGGTCGGTGGCTCGATCATGTTGGCTGTCCCGCCCGCCATTCTGGATATGCTGCGTCT
GCGTGCCGGCGCTACCGTTGGCTTGGCCGTCGATCATGGTCGCCTGGTGATCGAACCCACGCTGCGTCCGTACTACAGCC
TGGATGAGCTGCTGGCCCAATGCGACGCATCCGCCGAACTCTCCACCGAAGATCGGGAATGGCTCGACGCCAAGCCTGTT
GGCAGCGAGCTGCTGTGA

Structures

Toxin

• AlphaFold DB: M4U123

Antitoxin

• AlphaFold DB: M4UHU6