Detailed information of TA system TA070717

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): pemIK/PRK09812-MazE
• Location: 42876..43465
• Replicon: plasmid pLH276.2
• Accession: NZ_CP018856
• Organism: Xanthomonas citri pv. citri strain LH276

Toxin (Protein)

• Gene name: pemK
• Uniprot ID: M4U123
• Locus tag: BI316_RS24645
• Protein ID: WP_015463824.1
• Coordinates: 42876..43208 (-)
• Length: 111 a.a.

Antitoxin (Protein)

• Gene name: pemI
• Uniprot ID: M4UHU6
• Locus tag: BI316_RS24650
• Protein ID: WP_011052949.1
• Coordinates: 43208..43465 (-)
• Length: 86 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BI316_RS26395	41356..41577	-	222	WP_011052944.1	hypothetical protein	-
BI316_RS24635	41867..42403	-	537	WP_015463823.1	plasmid pRiA4b ORF-3 family protein	-
BI316_RS24640	42393..42605	-	213	WP_011052947.1	hypothetical protein	-
BI316_RS26150	42602..42853	-	252	WP_125459571.1	hypothetical protein	-
BI316_RS24645	42876..43208	-	333	WP_015463824.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
BI316_RS24650	43208..43465	-	258	WP_011052949.1	antitoxin	Antitoxin
BI316_RS24655	43671..44273	+	603	WP_011052950.1	recombinase family protein	-
BI316_RS24660	44257..44561	+	305	Protein_47	Tn3 family transposase	-
BI316_RS24665	44728..44934	-	207	Protein_48	Tn3 family transposase	-
BI316_RS24670	44979..45548	-	570	Protein_49	UPF0149 family protein	-
BI316_RS24675	45567..46781	-	1215	WP_011050083.1	IS4-like element ISXac1 family transposase	-
BI316_RS26155	46855..47037	-	183	Protein_51	UPF0149 family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..73024	73024

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (3-108)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 111 a.a.
• Molecular weight: 11707.46 Da
• Isoelectric Point: 9.1975

>T70717 WP_015463824.1 NZ_CP018856:c43208-42876 [Xanthomonas citri pv. citri]
MDRGDVYLVSLDPTSGHEQQGTRPVLIVSPSAFNRLTKTPVVLPITSGGNFARTAGFTVSLMGAGTNTTGVVRCDQPRAL
DLASRRARKLETVPSPIMDEVLAKLSAILE

Nucleotide

• Length: 333 bp

>T70717 NZ_CP018856:c43208-42876 [Xanthomonas citri pv. citri]
ATGGATCGCGGCGATGTCTATTTGGTTTCGCTCGATCCCACGTCCGGCCATGAACAACAGGGCACGCGCCCGGTGCTGAT
TGTGTCGCCAAGCGCGTTCAACCGCCTGACGAAAACACCGGTCGTGCTGCCGATCACCAGTGGGGGTAACTTCGCCCGCA
CGGCAGGCTTTACCGTGTCGCTCATGGGTGCGGGTACCAACACAACTGGCGTAGTTCGCTGCGATCAGCCCCGCGCTCTC
GATCTAGCCTCGCGGCGCGCGCGCAAGCTGGAAACCGTGCCGTCCCCGATCATGGATGAGGTATTAGCGAAGCTGAGCGC
GATCCTTGAGTAA

Antitoxin

Protein

• Length: 86 a.a.
• Molecular weight: 9242.70 Da
• Isoelectric Point: 4.7164

>AT70717 WP_011052949.1 NZ_CP018856:c43465-43208 [Xanthomonas citri pv. citri]
MHTTNLRKVGGSIMLAVPPAILDMLRLRAGATVGLAVDHGRLVIEPTLRPYYSLDELLAQCDASAELSTEDREWLDAKPV
GSELL

Nucleotide

• Length: 258 bp

>AT70717 NZ_CP018856:c43465-43208 [Xanthomonas citri pv. citri]
ATGCATACGACCAATCTGCGTAAGGTCGGTGGCTCGATCATGTTGGCTGTCCCGCCCGCCATTCTGGATATGCTGCGTCT
GCGTGCCGGCGCTACCGTTGGCTTGGCCGTCGATCATGGTCGCCTGGTGATCGAACCCACGCTGCGTCCGTACTACAGCC
TGGATGAGCTGCTGGCCCAATGCGACGCATCCGCCGAACTCTCCACCGAAGATCGGGAATGGCTCGACGCCAAGCCTGTT
GGCAGCGAGCTGCTGTGA

Structures

Toxin

• AlphaFold DB: M4U123

Antitoxin

• AlphaFold DB: M4UHU6