Detailed information of TA system TA070701

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/PemK-MazE
• Location: 16249..16790
• Replicon: plasmid pLL074-4.2
• Accession: NZ_CP018849
• Organism: Xanthomonas citri pv. citri strain LL074-4

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: BI314_RS24595
• Protein ID: WP_173667216.1
• Coordinates: 16464..16790 (+)
• Length: 109 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: H8FMA7
• Locus tag: BI314_RS24590
• Protein ID: WP_003491150.1
• Coordinates: 16249..16467 (+)
• Length: 73 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BI314_RS24565	11962..13182	+	1221	WP_011052984.1	TrbI/VirB10 family protein	-
BI314_RS24570	13193..14215	+	1023	WP_011052983.1	P-type DNA transfer ATPase VirB11	-
BI314_RS24575	14226..15104	+	879	WP_011052982.1	transglycosylase SLT domain-containing protein	-
BI314_RS24580	15230..15649	+	420	WP_011052981.1	hypothetical protein	-
BI314_RS24585	15726..16112	+	387	WP_003491149.1	hypothetical protein	-
BI314_RS24590	16249..16467	+	219	WP_003491150.1	antitoxin MazE family protein	Antitoxin
BI314_RS24595	16464..16790	+	327	WP_173667216.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
BI314_RS24600	16844..19819	-	2976	WP_011052978.1	conjugative relaxase	-
BI314_RS24605	19833..21407	-	1575	WP_011052977.1	type IV secretion system DNA-binding domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..92708	92708

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (3-103)

Antitoxin

• Antitoxin MazE-like (5-70)

Sequences

Toxin

Protein

• Length: 109 a.a.
• Molecular weight: 11677.64 Da
• Isoelectric Point: 7.1664

>T70701 WP_173667216.1 NZ_CP018849:16464-16790 [Xanthomonas citri pv. citri]
MMRGDFVTIAMQGDFGEPRPALVIQADQFDAHTTVTVLPVTSTLVAAPLLRITVHPSTDNGLQKPSQVMVDKAMTVKRDK
VGRAFGRVDADALVEIERCLAVFLGIAK

Nucleotide

• Length: 327 bp

>T70701 NZ_CP018849:16464-16790 [Xanthomonas citri pv. citri]
ATGATGCGGGGTGACTTTGTGACCATCGCCATGCAAGGCGACTTCGGAGAACCAAGGCCCGCTCTGGTGATCCAGGCCGA
CCAGTTTGACGCACACACCACCGTGACGGTGCTGCCGGTGACAAGCACGCTCGTTGCTGCGCCGCTGCTGCGTATTACCG
TTCATCCAAGTACCGACAATGGTTTGCAGAAGCCGTCTCAGGTGATGGTGGACAAGGCCATGACCGTGAAGCGTGACAAG
GTAGGTCGAGCCTTTGGGCGCGTAGACGCTGATGCGTTGGTGGAAATTGAGCGCTGCCTGGCCGTGTTCTTGGGCATCGC
TAAGTGA

Antitoxin

Protein

• Length: 73 a.a.
• Molecular weight: 8418.54 Da
• Isoelectric Point: 5.6599

>AT70701 WP_003491150.1 NZ_CP018849:16249-16467 [Xanthomonas citri pv. citri]
MAHVNSRVQKHRDALRMAGLRPVQIWVPDTRRPDFAEECRRQCRLAAQADMADTDMQRFMDEALADMDGWTE

Nucleotide

• Length: 219 bp

>AT70701 NZ_CP018849:16249-16467 [Xanthomonas citri pv. citri]
ATGGCACACGTCAATTCACGAGTTCAAAAGCACCGCGACGCCCTGCGCATGGCAGGGCTGCGTCCGGTGCAAATTTGGGT
GCCGGACACACGGCGGCCTGACTTCGCCGAGGAATGCCGCCGTCAGTGTCGTCTTGCTGCACAAGCGGACATGGCGGATA
CCGACATGCAGCGCTTCATGGATGAGGCGCTAGCAGACATGGATGGCTGGACGGAATGA

Structures

Antitoxin

• AlphaFold DB: H8FMA7