Detailed information of TA system TA070693

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/HicA-HicB
• Location: 509108..509739
• Replicon: chromosome
• Accession: NZ_CP018846
• Organism: Pseudomonas putida strain AA7

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: -
• Locus tag: BUQ73_RS02380
• Protein ID: WP_079226515.1
• Coordinates: 509108..509335 (+)
• Length: 76 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: BUQ73_RS02385
• Protein ID: WP_060485575.1
• Coordinates: 509332..509739 (+)
• Length: 136 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BUQ73_RS02355	505444..506421	-	978	WP_079226511.1	hypothetical protein	-
BUQ73_RS02360	506432..506920	-	489	WP_079226512.1	type VI secretion system tube protein Hcp	-
BUQ73_RS02365	507218..507562	-	345	WP_003258412.1	cupin domain-containing protein	-
BUQ73_RS02370	507659..508588	-	930	WP_079226513.1	CDF family Co(II)/Ni(II) efflux transporter DmeF	-
BUQ73_RS02375	508659..508934	+	276	WP_079226514.1	metal/formaldehyde-sensitive transcriptional repressor	-
BUQ73_RS02380	509108..509335	+	228	WP_079226515.1	type II toxin-antitoxin system HicA family toxin	Toxin
BUQ73_RS02385	509332..509739	+	408	WP_060485575.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
BUQ73_RS02390	509850..510734	-	885	WP_079226516.1	LysR family transcriptional regulator	-
BUQ73_RS02395	510813..511577	+	765	WP_079230468.1	sulfite exporter TauE/SafE family protein	-
BUQ73_RS02400	511567..511881	-	315	WP_027918390.1	antibiotic biosynthesis monooxygenase	-
BUQ73_RS02405	512040..512630	+	591	WP_079226517.1	NAD(P)H-dependent oxidoreductase	-
BUQ73_RS02410	512661..513557	+	897	WP_079226518.1	LysR family transcriptional regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (3-59)

Antitoxin

• HicB-like antitoxin of toxin-antitoxin system (3-127)

Sequences

Toxin

Protein

• Length: 76 a.a.
• Molecular weight: 8322.39 Da
• Isoelectric Point: 10.2063

>T70693 WP_079226515.1 NZ_CP018846:509108-509335 [Pseudomonas putida]
MRSREVIQLIEADGWYEAEVKGSHHQFRHPMKKGRVTVPHPKSDLPKGTVHSILKQAGLKQSSGFDSSNISGDAQ

Nucleotide

• Length: 228 bp

>T70693 NZ_CP018846:509108-509335 [Pseudomonas putida]
ATGCGCAGTCGTGAAGTGATCCAGCTCATTGAGGCGGACGGGTGGTACGAGGCCGAGGTCAAGGGCAGCCATCACCAGTT
CAGGCACCCGATGAAGAAGGGGAGGGTGACAGTTCCTCATCCCAAGAGCGATCTTCCTAAAGGCACCGTACACAGCATCC
TGAAGCAAGCTGGCTTGAAGCAGTCCAGTGGCTTCGATAGCTCGAACATCAGTGGAGACGCGCAATGA

Antitoxin

Protein

• Length: 136 a.a.
• Molecular weight: 14605.40 Da
• Isoelectric Point: 4.7169

>AT70693 WP_060485575.1 NZ_CP018846:509332-509739 [Pseudomonas putida]
MKFPVVLHKDADSDYGVTVPDVPGCFSAGSTVAQALENVQEALALHFEGLVADNEALPQAQEVDVHVANSDYAGGVWAVV
DFDVTPYLGKAVRFNATLPENLLQRIDEKVKRDHRYASRSGFLASAALRELALAH

Nucleotide

• Length: 408 bp

>AT70693 NZ_CP018846:509332-509739 [Pseudomonas putida]
ATGAAATTCCCGGTCGTATTGCACAAGGACGCCGATTCGGATTATGGCGTGACCGTTCCCGACGTGCCTGGTTGCTTCTC
TGCGGGCTCGACTGTCGCCCAGGCGCTGGAGAACGTTCAAGAGGCGCTAGCGCTGCATTTTGAGGGTTTGGTTGCTGATA
ACGAAGCGCTTCCTCAAGCGCAGGAAGTCGACGTCCATGTGGCGAATTCCGATTATGCTGGCGGGGTCTGGGCTGTCGTT
GATTTCGATGTCACGCCTTACTTGGGGAAGGCCGTGCGGTTCAACGCAACATTGCCGGAAAACCTCCTTCAGCGGATCGA
TGAAAAAGTGAAGCGTGACCATCGGTATGCTTCTCGTTCTGGATTTCTCGCGTCGGCCGCTCTAAGAGAACTGGCGCTCG
CACACTGA