Detailed information of TA system TA070686

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 503935..504464
• Replicon: chromosome
• Accession: NZ_CP018842
• Organism: Staphylococcus epidermidis strain 14.1.R1

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: A0A939SRT1
• Locus tag: BUM85_RS02560
• Protein ID: WP_002447757.1
• Coordinates: 504102..504464 (+)
• Length: 121 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: Q5HME6
• Locus tag: BUM85_RS02555
• Protein ID: WP_001829931.1
• Coordinates: 503935..504105 (+)
• Length: 57 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BUM85_RS02530	499759..500238	+	480	WP_002447762.1	PH domain-containing protein	-
BUM85_RS02535	500231..501736	+	1506	WP_002447761.1	PH domain-containing protein	-
BUM85_RS02540	501723..502241	+	519	WP_002447760.1	PH domain-containing protein	-
BUM85_RS02545	502280..502633	+	354	WP_002447759.1	holo-ACP synthase	-
BUM85_RS02550	502700..503848	+	1149	WP_002475027.1	alanine racemase	-
BUM85_RS02555	503935..504105	+	171	WP_001829931.1	type II toxin-antitoxin system antitoxin MazE	Antitoxin
BUM85_RS02560	504102..504464	+	363	WP_002447757.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
BUM85_RS02565	504810..505811	+	1002	WP_002447756.1	PP2C family protein-serine/threonine phosphatase	-
BUM85_RS02570	505911..506237	+	327	WP_001829952.1	anti-sigma factor antagonist	-
BUM85_RS02575	506239..506717	+	479	Protein_505	anti-sigma B factor RsbW	-
BUM85_RS02580	506692..507462	+	771	WP_002458689.1	RNA polymerase sigma factor SigB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (3-109)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 121 a.a.
• Molecular weight: 13483.62 Da
• Isoelectric Point: 9.9522

>T70686 WP_002447757.1 NZ_CP018842:504102-504464 [Staphylococcus epidermidis]
MIRRGDVYLADLSPVQGSEQGGVRPVVIIQNDTGNKYSPTVIVAAITGRINKAKIPTHVEIEKKKYKLDKDSVILLEQIR
TLDKKRLKEKLTFLSDSKMIEVDNALDISLGLNNFDHHKS

Nucleotide

• Length: 363 bp

>T70686 NZ_CP018842:504102-504464 [Staphylococcus epidermidis]
ATGATTAGAAGAGGAGATGTTTATTTAGCGGATTTATCACCAGTTCAAGGGTCTGAACAAGGGGGAGTAAGACCTGTAGT
TATCATTCAAAATGATACTGGTAATAAATATAGTCCAACTGTAATTGTAGCTGCGATTACTGGTAGGATTAATAAAGCGA
AAATACCAACCCACGTAGAAATTGAAAAGAAAAAGTATAAATTAGATAAAGATTCAGTTATTCTTCTTGAACAAATTAGA
ACACTAGATAAAAAGCGTTTAAAAGAAAAATTAACATTTTTATCAGATAGTAAAATGATAGAGGTTGATAATGCCTTAGA
TATTAGTTTGGGATTAAATAACTTTGATCATCATAAATCTTAA

Antitoxin

Protein

• Length: 57 a.a.
• Molecular weight: 6289.92 Da
• Isoelectric Point: 4.1920

>AT70686 WP_001829931.1 NZ_CP018842:503935-504105 [Staphylococcus epidermidis]
MLSFNQNRNHSLEQSLKEGYAQMADLNLSLATEAFPIECEACDCNESHLISNSKNE

Nucleotide

• Length: 171 bp

>AT70686 NZ_CP018842:503935-504105 [Staphylococcus epidermidis]
ATGTTATCTTTTAATCAAAATAGAAACCACAGTCTTGAACAATCTTTAAAAGAAGGTTATGCACAAATGGCCGATTTAAA
CCTCTCCCTAGCAACAGAAGCTTTCCCGATAGAGTGTGAAGCTTGTGATTGCAACGAATCACATTTAATATCTAATTCCA
AGAATGAATGA

Structures

Antitoxin

• AlphaFold DB: A0A0N1EF65