Detailed information of TA system TA070672

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): itaRT/DUF1778(antitoxin)
• Location: 2857628..2858465
• Replicon: chromosome
• Accession: NZ_CP018840
• Organism: Escherichia coli strain KSC64

Toxin (Protein)

• Gene name: itaT
• Uniprot ID: Q3Z4X7
• Locus tag: BUQ71_RS14410
• Protein ID: WP_000227784.1
• Coordinates: 2857628..2858170 (-)
• Length: 181 a.a.

Antitoxin (Protein)

• Gene name: itaR
• Uniprot ID: I2UQS9
• Locus tag: BUQ71_RS14415
• Protein ID: WP_001297137.1
• Coordinates: 2858154..2858465 (-)
• Length: 104 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BUQ71_RS14390	2852909..2853400	+	492	WP_001138904.1	nucleotide binding protein YajQ	-
BUQ71_RS14395	2853528..2854892	-	1365	WP_001000974.1	MFS transporter	-
BUQ71_RS14400	2855266..2856474	-	1209	WP_001352368.1	IS4-like element ISVsa5 family transposase	-
BUQ71_RS14405	2856637..2857572	+	936	WP_001377877.1	sel1 repeat family protein	-
BUQ71_RS14410	2857628..2858170	-	543	WP_000227784.1	GNAT family N-acetyltransferase	Toxin
BUQ71_RS14415	2858154..2858465	-	312	WP_001297137.1	DUF1778 domain-containing protein	Antitoxin
BUQ71_RS14420	2858650..2859540	-	891	WP_000971336.1	heme o synthase	-
BUQ71_RS14425	2859552..2859881	-	330	WP_000019869.1	cytochrome o ubiquinol oxidase subunit IV	-
BUQ71_RS14430	2859881..2860495	-	615	WP_000179819.1	cytochrome o ubiquinol oxidase subunit III	-
BUQ71_RS14435	2860485..2862476	-	1992	WP_000467180.1	cytochrome o ubiquinol oxidase subunit I	-
BUQ71_RS14440	2862498..2863445	-	948	WP_001239441.1	cytochrome o ubiquinol oxidase subunit II	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	flank	IS/Tn	-	-	2855266..2856474	1208

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (68-156)

Antitoxin

• Vibrio phage ICP1, Orf50 (14-97)

Sequences

Toxin

Protein

• Length: 181 a.a.
• Molecular weight: 19805.02 Da
• Isoelectric Point: 8.3395

>T70672 WP_000227784.1 NZ_CP018840:c2858170-2857628 [Escherichia coli]
MVDKHEEITLPIVLSCNYQSDITYPGQKQFDCGNPVIDKFVRASLKKSVRNSDCAAKALIDRQSGELIGICTFTAYSLEK
QRVSGVLQGSQPSEIGVVRLVMLGVARKYQKRGFGQDLLCDFFEHVKIIHQALPIKGVYLDADPAAINFYARLGFVQLSA
TPNAFGAVPMFLAIQHILAA

Nucleotide

• Length: 543 bp

>T70672 NZ_CP018840:c2858170-2857628 [Escherichia coli]
ATGGTCGACAAGCATGAAGAGATTACTCTGCCCATAGTCCTCTCCTGTAATTATCAGTCTGATATTACTTATCCTGGGCA
AAAACAGTTTGATTGCGGTAACCCTGTTATCGATAAATTTGTACGCGCATCGCTAAAGAAAAGTGTGCGTAATAGCGACT
GTGCGGCTAAAGCACTTATTGACAGACAAAGTGGTGAACTGATCGGCATCTGTACTTTTACGGCATATTCGCTGGAAAAA
CAACGCGTTTCTGGCGTCCTTCAGGGTTCACAACCTTCAGAAATTGGTGTTGTCAGATTAGTCATGTTGGGGGTAGCACG
GAAGTATCAAAAGCGGGGCTTTGGTCAGGACCTACTATGTGATTTTTTTGAACATGTAAAAATAATTCACCAGGCATTAC
CAATTAAAGGGGTTTATCTTGATGCTGACCCTGCCGCCATTAATTTTTATGCTCGTCTCGGCTTTGTTCAGCTTTCAGCG
ACACCAAATGCTTTTGGTGCTGTACCTATGTTTTTGGCGATTCAGCATATTCTCGCGGCTTAG

Antitoxin

Protein

• Length: 104 a.a.
• Molecular weight: 11358.04 Da
• Isoelectric Point: 10.4825

>AT70672 WP_001297137.1 NZ_CP018840:c2858465-2858154 [Escherichia coli]
MPGKTATLPDVDKTLKNARVELKTSPDAKNKLREAAQAVGVDLSAFILSAAMERAESVLDNQRRRELSNQSWELMNQLIA
EPAQPTLALKALMKRKNSDGRQA

Nucleotide

• Length: 312 bp

>AT70672 NZ_CP018840:c2858465-2858154 [Escherichia coli]
ATGCCTGGAAAAACAGCCACTCTACCCGATGTCGATAAAACGCTGAAAAATGCGCGGGTCGAACTCAAAACTAGTCCCGA
CGCCAAAAACAAACTTCGTGAAGCTGCGCAAGCCGTTGGGGTAGATTTAAGCGCTTTTATTTTGAGCGCTGCTATGGAAC
GCGCCGAAAGTGTGCTTGATAACCAACGTCGCCGTGAGCTTTCGAATCAAAGCTGGGAACTGATGAACCAACTCATCGCT
GAACCTGCTCAACCGACGCTCGCCCTCAAGGCGTTAATGAAAAGGAAAAACAGCGATGGTCGACAAGCATGA

Structures

Toxin

• PDB: 7BYY

Antitoxin

• AlphaFold DB: A0A0E0Y6B3