Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ataRT/DUF1778(antitoxin)
Location 1304330..1305130 Replicon chromosome
Accession NZ_CP018840
Organism Escherichia coli strain KSC64

Toxin (Protein)


Gene name ataT Uniprot ID F4NNI0
Locus tag BUQ71_RS06790 Protein ID WP_000342449.1
Coordinates 1304330..1304857 (-) Length 176 a.a.

Antitoxin (Protein)


Gene name ataR Uniprot ID F4NNI1
Locus tag BUQ71_RS06795 Protein ID WP_001277108.1
Coordinates 1304864..1305130 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
BUQ71_RS06760 1299405..1300172 - 768 WP_000082110.1 high-affinity branched-chain amino acid ABC transporter ATP-binding protein LivG -
BUQ71_RS06765 1300169..1301446 - 1278 WP_000803799.1 branched chain amino acid ABC transporter permease LivM -
BUQ71_RS06770 1301443..1302369 - 927 WP_000003004.1 high-affinity branched-chain amino acid ABC transporter permease LivH -
BUQ71_RS06775 1302417..1303526 - 1110 WP_000827696.1 high-affinity branched-chain amino acid ABC transporter substrate-binding protein LivK -
BUQ71_RS06785 1303950..1304333 + 384 WP_000778795.1 aspartate 1-decarboxylase autocleavage activator PanM -
BUQ71_RS06790 1304330..1304857 - 528 WP_000342449.1 GNAT family N-acetyltransferase Toxin
BUQ71_RS06795 1304864..1305130 - 267 WP_001277108.1 DUF1778 domain-containing protein Antitoxin
BUQ71_RS06800 1305280..1306383 - 1104 WP_001021996.1 branched chain amino acid ABC transporter substrate-binding protein LivJ -
BUQ71_RS06805 1306654..1307508 - 855 WP_000130217.1 RNA polymerase sigma factor RpoH -
BUQ71_RS06810 1307753..1308811 - 1059 WP_001042003.1 permease-like cell division protein FtsX -
BUQ71_RS06815 1308804..1309472 - 669 WP_000617723.1 cell division ATP-binding protein FtsE -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(88-150)

Antitoxin

Vibrio phage ICP1, Orf50

(8-86)


Sequences

Toxin        


Download         Length: 176 a.a.        Molecular weight: 19691.70 Da        Isoelectric Point: 7.7457

>T70668 WP_000342449.1 NZ_CP018840:c1304857-1304330 [Escherichia coli]
MDDLTIEILTDDADYDLQRFDCGEEALNLFLTTHLVRQHRNKILRAYILCRNTPERQVLGYYTLCGSCFERAALPSKSKQ
KKIPYKNIPSVTLGRLAIDRSLQGQGWGATLVAHAMKVVWSASLAVGIHGLFVEALNKKAHTFYQSLGFIPLVGENENAL
FFPTKSIELLFTQSD

Download         Length: 528 bp

>T70668 NZ_CP018840:c1304857-1304330 [Escherichia coli]
ATGGATGATCTGACGATAGAGATTCTGACCGATGATGCAGATTATGATCTACAGCGATTCGACTGCGGCGAGGAAGCGTT
AAATCTCTTTCTGACGACACATCTCGTTCGTCAACATCGCAACAAAATTCTGCGAGCGTATATCCTTTGTCGCAACACTC
CAGAACGTCAGGTGCTGGGATATTACACATTATGCGGCAGTTGTTTTGAACGAGCCGCATTGCCCTCGAAATCGAAACAG
AAAAAAATTCCCTACAAAAATATTCCCAGCGTTACTCTTGGGCGTCTGGCAATTGATCGTTCATTACAGGGGCAGGGATG
GGGAGCAACACTGGTTGCTCATGCCATGAAAGTCGTCTGGTCAGCCTCTTTAGCGGTAGGTATTCACGGTCTTTTTGTCG
AGGCGCTGAATAAAAAAGCCCATACGTTTTATCAATCGCTGGGCTTTATCCCGTTGGTCGGAGAAAACGAAAATGCGTTA
TTTTTCCCAACCAAATCCATCGAACTGCTTTTCACACAGAGCGATTAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 9910.31 Da        Isoelectric Point: 10.0863

>AT70668 WP_001277108.1 NZ_CP018840:c1305130-1304864 [Escherichia coli]
MSAVKKQRIDLRLTDDDKSMIEEAAAISNQSVSQFMLNSASQRAAEVIEQHRRVILNEESWTRVMDALSNPPSPGEKLKR
AAKRLQGM

Download         Length: 267 bp

>AT70668 NZ_CP018840:c1305130-1304864 [Escherichia coli]
ATGTCTGCTGTTAAAAAGCAGCGTATCGATCTGCGTTTAACTGACGATGACAAAAGTATGATTGAGGAAGCGGCAGCGAT
ATCTAATCAGTCCGTCAGCCAATTTATGTTGAACAGCGCTTCGCAGCGGGCTGCGGAAGTGATTGAACAGCATCGGCGGG
TGATCCTCAATGAGGAATCCTGGACGCGGGTGATGGATGCGCTGAGTAATCCACCGTCACCTGGTGAAAAGCTAAAACGT
GCGGCAAAACGTCTTCAGGGAATGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829CLZ4

Antitoxin

Source ID Structure
PDB 6GTS
PDB 6AJN
PDB 6GTQ
PDB 6GTO
PDB 6GTR
PDB 6AJM
AlphaFold DB A0A829CN24

References