Detailed information of TA system TA070665

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): rnlAB/RnlA-RnlB
• Location: 413699..415124
• Replicon: chromosome
• Accession: NZ_CP018840
• Organism: Escherichia coli strain KSC64

Toxin (Protein)

• Gene name: rnlA
• Uniprot ID: -
• Locus tag: BUQ71_RS02025
• Protein ID: WP_024190590.1
• Coordinates: 414054..415124 (-)
• Length: 357 a.a.

Antitoxin (Protein)

• Gene name: rnlB
• Uniprot ID: E6BSF0
• Locus tag: BUQ71_RS02020
• Protein ID: WP_000461705.1
• Coordinates: 413699..414061 (-)
• Length: 121 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BUQ71_RS01985	408831..410492	+	1662	WP_047086987.1	DNA repair protein RecN	-
BUQ71_RS01990	410641..410982	+	342	WP_001203437.1	outer membrane protein assembly factor BamE	-
BUQ71_RS01995	411044..411334	-	291	WP_001117838.1	RnfH family protein	-
BUQ71_RS02000	411324..411761	-	438	WP_001341638.1	type II toxin-antitoxin system toxin RatA	-
BUQ71_RS02005	411932..412414	+	483	WP_000162574.1	SsrA-binding protein SmpB	-
BUQ71_RS02015	413292..413561	+	270	WP_000429347.1	hypothetical protein	-
BUQ71_RS02020	413699..414061	-	363	WP_000461705.1	type II toxin-antitoxin system RnlB family antitoxin	Antitoxin
BUQ71_RS02025	414054..415124	-	1071	WP_024190590.1	type II toxin-antitoxin system RnlA family toxin	Toxin
BUQ71_RS02030	415184..415339	-	156	WP_001597763.1	hypothetical protein	-
BUQ71_RS02035	415525..416109	-	585	WP_023363231.1	tail fiber assembly protein	-
BUQ71_RS02040	416109..419135	-	3027	WP_087514502.1	prophage tail fiber N-terminal domain-containing protein	-
BUQ71_RS02045	419200..419799	-	600	WP_001230356.1	Ail/Lom family outer membrane beta-barrel protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Bacterial toxin, RNase RnlA/LsoA, N-terminal repeated domain (98-183)

Antitoxin

• Antitoxin RnlB/LsoB (15-112)

Sequences

Toxin

Protein

• Length: 357 a.a.
• Molecular weight: 40475.40 Da
• Isoelectric Point: 7.2332

>T70665 WP_024190590.1 NZ_CP018840:c415124-414054 [Escherichia coli]
MTVRNYTNLNLDRTTIEATSRAFIENKNYSVHSIGPMPGARAGLRVVFAKPGEALATVNIFYNNGGTSTVQYQTGANRNL
GKELADNLYETINPAEFEQVNMVLQGFVEANVLSVLQLSAEQPHIQFYEYLRNTHTTVWKIHSPEFQDELTVSLHHRNGT
LQIQGRPLSCYRVFIFNLSELLDLQGLEKVLIRQDDGKAYIVQKEVARSHLECEMGDAYPLLHKNVEKLLVSGLCVKLAA
PDLPDYCMLLYPELRSVEGVLKSTMYRYGMPITQDGFGPYFDKIGSEFILKQQFGRNLSSAKVKTINDAYTFFNRERHGL
FHMEIVVDTSRMVSDMARLMTKARQAWGIIKDLYIV

Nucleotide

• Length: 1071 bp

>T70665 NZ_CP018840:c415124-414054 [Escherichia coli]
ATGACAGTCAGGAACTACACTAATTTAAATTTAGATCGGACAACTATTGAGGCTACGTCTAGGGCATTTATTGAAAATAA
AAACTATTCCGTCCATTCAATTGGACCAATGCCTGGTGCAAGAGCAGGACTTCGGGTTGTGTTTGCTAAACCAGGAGAAG
CTTTGGCAACTGTTAACATTTTCTATAATAACGGAGGGACGTCTACAGTCCAGTATCAAACAGGAGCTAATCGTAATCTT
GGAAAAGAGTTGGCTGATAATCTCTATGAAACCATCAATCCTGCAGAATTTGAACAGGTAAATATGGTGTTACAAGGGTT
CGTTGAAGCGAACGTTTTATCTGTACTTCAGCTCTCAGCAGAGCAGCCACATATACAATTTTACGAATACTTACGGAACA
CTCATACAACGGTGTGGAAAATTCATTCGCCAGAATTTCAGGATGAGTTGACAGTTAGTCTGCATCACAGAAATGGAACA
CTACAAATACAAGGGCGTCCATTGTCATGTTATAGAGTGTTTATATTCAACTTGTCAGAGCTACTCGATTTACAAGGACT
GGAAAAAGTTCTCATTCGTCAGGACGATGGCAAAGCGTATATAGTTCAGAAAGAAGTTGCTCGCTCACATCTAGAGTGTG
AAATGGGGGATGCGTACCCACTATTGCATAAGAATGTTGAAAAATTGCTCGTTTCTGGGCTGTGTGTTAAACTCGCCGCC
CCCGATTTACCCGATTACTGTATGTTGTTGTATCCTGAATTACGTTCAGTTGAGGGAGTACTTAAATCAACAATGTACCG
ATATGGCATGCCAATCACGCAGGATGGTTTCGGGCCTTATTTTGATAAAATTGGGAGTGAATTTATTCTGAAACAACAAT
TTGGACGCAATTTATCGTCAGCTAAGGTTAAGACCATTAATGATGCCTATACTTTCTTTAACAGAGAACGTCATGGCCTG
TTTCATATGGAGATAGTTGTCGACACAAGCCGTATGGTTTCTGACATGGCACGACTGATGACTAAGGCCAGACAGGCATG
GGGAATTATTAAGGATTTGTATATTGTTTGA

Antitoxin

Protein

• Length: 121 a.a.
• Molecular weight: 13304.23 Da
• Isoelectric Point: 4.2386

>AT70665 WP_000461705.1 NZ_CP018840:c414061-413699 [Escherichia coli]
MFEITGVNVSGALIAVVMATGYENPLTSVHEIEKRLSVILGNSQATGEVLFDLLCSNGPEWNRFVSLEIRCGSILLDTAK
IVDEQEIPAQILRHQRDVLRNHPEYVEDSVLTPDDVLCIL

Nucleotide

• Length: 363 bp

>AT70665 NZ_CP018840:c414061-413699 [Escherichia coli]
TTGTTTGAAATAACAGGTGTTAACGTCTCAGGGGCTCTCATTGCCGTCGTTATGGCGACTGGCTACGAGAACCCCTTGAC
TTCAGTTCACGAAATCGAGAAGAGGCTTTCTGTTATACTGGGCAATTCACAGGCTACAGGTGAGGTTTTGTTTGACCTTT
TGTGTTCTAATGGTCCAGAGTGGAATCGCTTTGTTTCGCTGGAAATAAGGTGTGGCAGTATTTTGCTGGATACTGCCAAA
ATTGTGGATGAGCAAGAAATTCCAGCGCAGATCTTGAGACATCAGAGAGATGTATTACGTAACCACCCTGAGTACGTAGA
AGACTCTGTGTTAACACCAGATGATGTTCTTTGTATTCTCTAG

Structures

Antitoxin

• AlphaFold DB: A0A1X9TDS3