Detailed information of TA system TA070663

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-StbC
• Location: 2403370..2403998
• Replicon: chromosome
• Accession: NZ_CP018839
• Organism: Thauera chlorobenzoica strain 3CB1

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: A0A1H5RZ37
• Locus tag: Tchl_RS11150
• Protein ID: WP_075148479.1
• Coordinates: 2403582..2403998 (+)
• Length: 139 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: -
• Locus tag: Tchl_RS11145
• Protein ID: WP_075148478.1
• Coordinates: 2403370..2403582 (+)
• Length: 71 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
Tchl_RS11125	2398635..2399222	+	588	WP_075148474.1	biliverdin-producing heme oxygenase	-
Tchl_RS11130	2399219..2401231	+	2013	WP_075148475.1	diguanylate cyclase	-
Tchl_RS11135	2401292..2401897	-	606	WP_075148476.1	glutathione transferase GstA	-
Tchl_RS11140	2401929..2403200	-	1272	WP_075148477.1	bifunctional O-acetylhomoserine aminocarboxypropyltransferase/cysteine synthase	-
Tchl_RS11145	2403370..2403582	+	213	WP_075148478.1	DNA-binding protein	Antitoxin
Tchl_RS11150	2403582..2403998	+	417	WP_075148479.1	type II toxin-antitoxin system VapC family toxin	Toxin
Tchl_RS11155	2404006..2404401	+	396	WP_075148480.1	DUF423 domain-containing protein	-
Tchl_RS11160	2404630..2405820	+	1191	WP_075148481.1	class I SAM-dependent methyltransferase	-
Tchl_RS11165	2405960..2407120	-	1161	WP_075148482.1	response regulator	-
Tchl_RS11170	2407184..2407540	-	357	WP_075148483.1	Hpt domain-containing protein	-
Tchl_RS11175	2407618..2408823	-	1206	WP_075148484.1	EAL domain-containing response regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (2-124)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 139 a.a.
• Molecular weight: 15272.44 Da
• Isoelectric Point: 5.5702

>T70663 WP_075148479.1 NZ_CP018839:2403582-2403998 [Thauera chlorobenzoica]
MYLLDTNIISELRKGACANAGVTAFFATLAAEDIYLSVQTIGELRRGLEHIRHRGDREQAARLETWLDGVVAGYADRILG
FDVDCAQLWGSLMSPSPQHPIDKQIAAIALIHDLMVVTRNTDDFRPTGVRCINPFCSN

Nucleotide

• Length: 417 bp

>T70663 NZ_CP018839:2403582-2403998 [Thauera chlorobenzoica]
ATGTACTTGCTTGACACCAACATCATCAGTGAGCTGCGCAAGGGAGCATGTGCCAATGCCGGCGTGACCGCCTTTTTTGC
GACCCTGGCAGCGGAGGACATTTATCTGTCGGTGCAGACCATTGGCGAGCTTCGCCGCGGCCTCGAGCACATCCGTCACC
GCGGCGACCGCGAGCAGGCCGCCCGACTCGAGACCTGGCTGGATGGCGTGGTTGCCGGCTACGCAGACCGTATCCTGGGT
TTTGACGTCGACTGTGCTCAGCTTTGGGGTTCGCTCATGTCGCCCAGTCCACAGCATCCCATCGACAAGCAGATTGCCGC
CATCGCCCTCATTCATGACCTGATGGTCGTCACCCGTAACACGGACGACTTCAGGCCGACCGGTGTGCGTTGCATCAATC
CGTTCTGCTCGAACTGA

Antitoxin

Protein

• Length: 71 a.a.
• Molecular weight: 7640.75 Da
• Isoelectric Point: 10.4538

>AT70663 WP_075148478.1 NZ_CP018839:2403370-2403582 [Thauera chlorobenzoica]
MATSLVVRNIEPAVAQALKEAAARHGRSAEAEHREILRAALARPVRRTFKDVLASMPDVGADKDFNCRQG

Nucleotide

• Length: 213 bp

>AT70663 NZ_CP018839:2403370-2403582 [Thauera chlorobenzoica]
ATGGCGACCAGTCTAGTCGTTCGAAACATCGAGCCCGCCGTCGCGCAAGCCCTGAAAGAGGCGGCGGCACGGCATGGCCG
AAGTGCCGAGGCAGAGCATCGGGAGATTCTGCGCGCGGCGCTCGCCCGACCGGTGCGCCGGACCTTCAAGGACGTGCTGG
CGAGCATGCCCGACGTGGGGGCCGATAAAGATTTCAATTGCAGGCAGGGCTGA

Structures

Toxin

• AlphaFold DB: A0A1H5RZ37