Detailed information of TA system TA070661

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tacT-ataR/DUF1778(antitoxin)
• Location: 918701..919464
• Replicon: chromosome
• Accession: NZ_CP018839
• Organism: Thauera chlorobenzoica strain 3CB1

Toxin (Protein)

• Gene name: tacT
• Uniprot ID: A0A1H5U632
• Locus tag: Tchl_RS04400
• Protein ID: WP_075147327.1
• Coordinates: 918701..919195 (-)
• Length: 165 a.a.

Antitoxin (Protein)

• Gene name: ataR
• Uniprot ID: A0A1H5U817
• Locus tag: Tchl_RS04405
• Protein ID: WP_075147328.1
• Coordinates: 919192..919464 (-)
• Length: 91 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
Tchl_RS04380	915225..916253	-	1029	WP_083945286.1	glycosyltransferase	-
Tchl_RS04385	916318..917745	-	1428	WP_075147324.1	hypothetical protein	-
Tchl_RS04390	917862..918269	-	408	WP_075147325.1	type II toxin-antitoxin system VapC family toxin	-
Tchl_RS04395	918288..918539	-	252	WP_075147326.1	AbrB family transcriptional regulator	-
Tchl_RS04400	918701..919195	-	495	WP_075147327.1	GNAT family N-acetyltransferase	Toxin
Tchl_RS04405	919192..919464	-	273	WP_075147328.1	DUF1778 domain-containing protein	Antitoxin
Tchl_RS04410	919613..920584	-	972	WP_075147329.1	GDP-L-fucose synthase	-
Tchl_RS04415	920631..920924	-	294	WP_075147330.1	type II toxin-antitoxin system RelE/ParE family toxin	-
Tchl_RS04420	920925..921158	-	234	WP_075147331.1	addiction module protein	-
Tchl_RS04425	921191..922315	-	1125	WP_075147332.1	GDP-mannose 4,6-dehydratase	-
Tchl_RS04430	922366..923100	-	735	WP_198158984.1	WecB/TagA/CpsF family glycosyltransferase	-
Tchl_RS04435	923126..924322	-	1197	WP_198158985.1	glycosyltransferase family 4 protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	fcl / gmd	915225..944864	29639

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (35-148)

Antitoxin

• Vibrio phage ICP1, Orf50 (3-87)

Sequences

Toxin

Protein

• Length: 165 a.a.
• Molecular weight: 17853.52 Da
• Isoelectric Point: 10.1812

>T70661 WP_075147327.1 NZ_CP018839:c919195-918701 [Thauera chlorobenzoica]
MSLHLSVPQPLAASHILDEFTCGEASLDEWLKRRARTNQVSGASRTFVVTDQDGRVHGYYAMAAGAVAHQAATSRVLRNM
PDPVPVMVLARLAVDHRAQGIKLGAALLQDAVNRAVAVSQNAGVRALLVHALHDRAKQFYEHYGFQASPQHPMTLMLRLN
AAKV

Nucleotide

• Length: 495 bp

>T70661 NZ_CP018839:c919195-918701 [Thauera chlorobenzoica]
ATGAGCTTGCATCTGAGCGTACCGCAACCACTCGCTGCCAGTCACATCCTGGACGAATTCACTTGTGGGGAAGCCAGCCT
CGATGAATGGCTCAAGCGCCGGGCACGGACCAACCAGGTTAGCGGCGCGAGCCGCACCTTTGTCGTCACGGATCAGGACG
GTCGTGTCCATGGTTACTACGCAATGGCCGCAGGTGCGGTTGCACACCAAGCGGCAACCAGCAGGGTGCTACGCAATATG
CCCGACCCGGTGCCGGTGATGGTTCTGGCCAGACTCGCCGTTGACCACCGTGCCCAGGGAATCAAACTCGGCGCCGCCCT
GCTTCAAGACGCTGTCAATCGGGCTGTAGCCGTGTCGCAGAACGCCGGCGTCCGAGCCTTGCTCGTCCATGCTCTTCACG
ACCGTGCCAAACAGTTTTACGAGCATTACGGCTTTCAGGCGTCCCCGCAGCATCCCATGACATTGATGCTGCGCCTGAAC
GCCGCCAAGGTTTGA

Antitoxin

Protein

• Length: 91 a.a.
• Molecular weight: 9988.53 Da
• Isoelectric Point: 5.0474

>AT70661 WP_075147328.1 NZ_CP018839:c919464-919192 [Thauera chlorobenzoica]
MRDAAINLRALPEQRDLIDHAASLLGKNRSDFMLEAACERAQAVVLDQVFFSLDGDKFKQFTAMLDAPPCPNLGLERLMA
VKAPWRTDAA

Nucleotide

• Length: 273 bp

>AT70661 NZ_CP018839:c919464-919192 [Thauera chlorobenzoica]
ATGCGCGACGCCGCCATCAATCTGCGGGCCCTGCCCGAACAGCGCGACCTGATCGATCACGCCGCCAGCCTGCTCGGCAA
AAACCGTTCGGACTTCATGCTCGAAGCGGCATGCGAGCGCGCTCAGGCTGTGGTGTTGGATCAGGTCTTTTTCAGCCTGG
ACGGTGACAAGTTCAAGCAGTTCACTGCCATGCTCGATGCGCCCCCCTGTCCTAATCTTGGGCTTGAACGCCTCATGGCC
GTCAAGGCCCCCTGGCGCACCGATGCAGCATGA

Structures

Toxin

• AlphaFold DB: A0A1H5U632

Antitoxin

• AlphaFold DB: A0A1H5U817